Background: Brucellosis can be well-defined as a bacterial zoonotic infection conveyed to humans by direct interaction with infected animals or its food products such as meat, cheese, and unpasteurized milk mainly domesticated ruminants and swine. Objective: Determination of the students’ knowledge about Brucellosis. Methods: Medical students of Jouf University asked to complete a questionnaire capture about their knowledge on the etiology, clinical feature, complications, prognosis and prevention of brucellosis. Results: The overall results showed that there is a lack in awareness about brucellosis among the students. Conclusion: The medical training institutions should give special consideration to brucellosis in their teaching curricula.