Hameed, T., Humud, H., Ali, L. (2023). Assessment of Plasma Jet Therapy of Tooth Root Canal Infected with Escherichia Coli and Enterococcus Faecalis Biofilm In Vitro. The Egyptian Journal of Hospital Medicine, 90(2), 2359-2362. doi: 10.21608/ejhm.2023.286010
Tamara A. Hameed; Hammad R. Humud; Layla F. Ali. "Assessment of Plasma Jet Therapy of Tooth Root Canal Infected with Escherichia Coli and Enterococcus Faecalis Biofilm In Vitro". The Egyptian Journal of Hospital Medicine, 90, 2, 2023, 2359-2362. doi: 10.21608/ejhm.2023.286010
Hameed, T., Humud, H., Ali, L. (2023). 'Assessment of Plasma Jet Therapy of Tooth Root Canal Infected with Escherichia Coli and Enterococcus Faecalis Biofilm In Vitro', The Egyptian Journal of Hospital Medicine, 90(2), pp. 2359-2362. doi: 10.21608/ejhm.2023.286010
Hameed, T., Humud, H., Ali, L. Assessment of Plasma Jet Therapy of Tooth Root Canal Infected with Escherichia Coli and Enterococcus Faecalis Biofilm In Vitro. The Egyptian Journal of Hospital Medicine, 2023; 90(2): 2359-2362. doi: 10.21608/ejhm.2023.286010
Assessment of Plasma Jet Therapy of Tooth Root Canal Infected with Escherichia Coli and Enterococcus Faecalis Biofilm In Vitro
Background: Common and persistent isolate ina the teeth following failed therapy of the root canal is the gram-positive facultative bacterium Enterococcus faecalis and Escherichia coli, which develop biofilm through a complicated process that results in the formation of a biofilm. Enterococcus faecalis and Escherichia coli are significant factors that cause chronic periradicular lesions after root canal therapy. Aim: This study aimed to treat the root canal tooth infected with Escherichia coli and Enterococcus faecalis Methods: In this study biofilm formation was done for Escherichia coli in growth phase cultured in a brain heart broth Enterococcus faecalis and Escherichia coli cultured in Luria-Bertani (LB) infusion medium for 18 hrs. Then we studied the effect of plasma jet that works with argon gas, and it is generated by a power supply that operates at alternating high voltages in the form of a sinusoidal wave with peak-to-peak value of about 12 kV at a frequency of 30 KHz and its power is about 200 watts. Results: Enterococcus faecalis biofilms were treated for 0.5, 1, 1.5, 2, 2.5 minute with a constant gas flow rate 2.5 . The killing rate decreased from (0.979 to 0.361). In the Enterococcus faecalis biofilms was treated for a longer period of time (5, 10, 15, 20, 25, 30) min, as the killing rates decreased from (0.739 to 0.179). Conclusions: The result of this study indicates that plasma jet is useful in disinfecting root canal tooth and reducing the formation of biofilm.
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