Molecular Research of The Difference between Chromosome and Plasmid at Harboring Some Virulence and Antibiotic Resistance Genes in P. Mirabilis

Owaied, Huda Qasim; Jabur, Sanaa Ghali

doi:10.21608/ejhm.2022.277399

Molecular Research of The Difference between Chromosome and Plasmid at Harboring Some Virulence and Antibiotic Resistance Genes in P. Mirabilis

Document Type : Original Article

Authors

10.21608/ejhm.2022.277399

Abstract

Background: Proteus spp < em>. is gram negative bacteria belonging to the Enterobacteriaceae family. P. mirabilis is the most commonly isolated species from clinical samples. Objective: The present study aimed to investigate and compare the prevalence of virulence genes (hpmA, hpmB) and the antibiotic resistant genes (bla_CTX-M and bla_KPC) on both chromosome and plasmid in Proteus mirabilis isolates. Material and methods: A total of 487 samples were collected from different clinical sources. Samples were obtained for patients of different ages were involved of both sexes. The samples were collected from hospitals and private laboratories in Thi-Qar province, Iraq during a period from November 2021 to Abril 2022. A total of the isolates were diagnosed by different laboratory and molecular methods. Results: The prevalence of P.mirabilis was 8.6 % among collected samples. The findings of virulence genes (hpmA and hpmB) indicated that 45% of isolates were positive for hpmA gene on chromosome and 33.3% on plasmid, while hpmB gene rate was 30.9% on chromosome and 16.7% on plasmid. On the other hand the antibiotic resistance genes (bla_CTX-M and bla_KPC) test results showed that the bla_CTX-M gene was absent on the chromosome with a very high frequency on the plasmid (95.2%). While the bla_KPCappeared in a low rate on the chromosome (38%) but it has been risen on the plasmid (95.2%). Conclusion: The examined virulence genes (hpmA, hpmB) were found mostly on the chromosome while the antibiotic resistance genes

(bla_CTX-M, bla_KPC) found mostly on the plasmid.

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