Chemoprevention of Barley and Sage against acrylamide-Induced genotoxic, biochemical and histopathological alterations in rats

Document Type : Original Article

Authors

1 Department of Cell Biololgy Faculty of Medicine for girls, Al Azhar University, Cairo, Egypt.

2 Department of Food Toxicology & Contaminants National Research Centre, Dokki, Cairo, Egypt.

3 Department of Pathology. Faculty of Vet. Med. Cairo Univ. Egypt

4 Forensic Medicine & Toxicology Department, Faculty of Medicine for girls, Al Azhar University, Cairo, Egypt.

Abstract

Acrylamide (ACR) has recently been found in fried and backed foods, suggesting widespread public exposure. ACR is an industrial chemical material that causes neurotoxicity in humans and was designated as a probable human carcinogen by IARC and USEPA. The aim of the present study was to evaluate the protective effects of barley and sage against ACR mutagenicity and biochemical and histopathological changes in rats. Forty mature male rats were divided into eight groups and were fed barley and/or sage-supplemented diet (5%) with or without ACR (50 mg/kg b.w). The biochemical results revealed that ACR increased Alanine amino transferase (ALT), Aspartate amino transferase (AST), triglycerides (TG), cholesterol and uric acid. Micronucleated polychromatic erythrocytes and chromosomal aberrations in somatic and germ cells were significantly increased in ACR-treated animals. Severe pathological lesions included testicular degeneration, oedema, spermatid giant cells formation and necrosis of spermatid cells were found in the testis of ACR-treated group. The kidney of this group showed degenerative changes. Cotreatment with barley and/or Sage and ACR resulted in a significant improvement in all the parameters tested. It could be concluded that these plants contain antioxidant compounds and may be useful when add as food additive to the food cooked on a higher temperature.
 

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