Aspergillus fumigatus and Cylindrocarpon candidum fungi induced apoptosis in HepG 2 cell line through activation of caspases enzymes

M.E, Osman,; O.H, Khttab,; A.A, Abo EL-Nasser,; Sallam, L,A,R,; Ahmed, Wafaa Abdallah

doi:10.21608/ejhm.2007.17719

Aspergillus fumigatus and Cylindrocarpon candidum fungi induced apoptosis in HepG 2 cell line through activation of caspases enzymes

Document Type : Original Article

Authors

¹ Department of Botany and Microbiology, Faculty of science, Helwan University.

² National Research Center, Department of Microbiology and Natural Products Chemistry

³ Department of Cancer Biology, Cancer Institute, Cairo University.

10.21608/ejhm.2007.17719

Abstract

Introduction: Many investigations are now interested to discover naturally occurring compounds, which can be used for the prevention and treatment of cancer. Most natural products which may be used as adjuvant therapy or to reduce the side effect of chemotherapy and radiotherapy. More than 300 products obtained from microorganisms have antitumor activities.
Results: In the study we isolated N-(3-4-Dichlorophenyl) 2-Methyl, 2,3Dihydroxypropio amide from Aspergillus fumigatus and 2.4.6. Triphenyl pyridine from Cylindrocarpon candidum and investigate the cytotoxic effect and apoptotic effect on HepG2 cell line. The results revealed high cytotoxic effect at the concentration of 400μg/ml for both N-(3-4- Dichlorophenyl) 2-Methyl, 2,3Dihydroxypropio amide and 2.4.6. Triphenyl pyridine and effect is increase with time of incubation. The apoptotic effect of both products were investigated by measurement the caspase enzymes, the results showed highest activity of caspase 3 and caspase 9. Also at concentration 400μg/ml in both products.
Conclusion: From this data we observe that two isolated product have antitumor effect and this effect is related to the concentration of the products and incubation period. Also, the two products induce apoptosis through increase activation of caspase 3 and caspase 9 which lead to programmed of cell death. This study need to furthermore study on experimental animal to confirm our results.