Sayed, A., El-Desoky, M., Shaarawy, S., Ashmawy, A. (2012). Changes Of P21 And Bcl-2 During Induction Of Bladder Inflammation And/Or Bladder Carcinogenesis. The Egyptian Journal of Hospital Medicine, 49(1), 781-792. doi: 10.21608/ejhm.2012.16214
A.A. Sayed; M.A. El-Desoky; S. Shaarawy; A.M. Ashmawy. "Changes Of P21 And Bcl-2 During Induction Of Bladder Inflammation And/Or Bladder Carcinogenesis". The Egyptian Journal of Hospital Medicine, 49, 1, 2012, 781-792. doi: 10.21608/ejhm.2012.16214
Sayed, A., El-Desoky, M., Shaarawy, S., Ashmawy, A. (2012). 'Changes Of P21 And Bcl-2 During Induction Of Bladder Inflammation And/Or Bladder Carcinogenesis', The Egyptian Journal of Hospital Medicine, 49(1), pp. 781-792. doi: 10.21608/ejhm.2012.16214
Sayed, A., El-Desoky, M., Shaarawy, S., Ashmawy, A. Changes Of P21 And Bcl-2 During Induction Of Bladder Inflammation And/Or Bladder Carcinogenesis. The Egyptian Journal of Hospital Medicine, 2012; 49(1): 781-792. doi: 10.21608/ejhm.2012.16214
Changes Of P21 And Bcl-2 During Induction Of Bladder Inflammation And/Or Bladder Carcinogenesis
1M.Sc candidate, Faculty of Science, Cairo University
2Department of Chemistry,BiochemistryDivision, Faculty of Science, Cairo University
3Department of Medical Biochemistry,NCI , Cairo University
Abstract
The effect of E.coli infection in association with dibutylamine and sodium nitrate in induction of bladder inflammation and /or bladder carcinogenesis were investigated in 150 male albino rats, divided into five groups, as follows: The first group(G1) infected by E. coli , The second group (G2), given nitrosamine precursors in the diet , The third group(G3) infected by E. coli and given nitrosamine precursors in the diet ,The fourth group(G4) infected by E. coli , given nitrosamine precursors in the diet and received standard diet containing 1% Curcumin powder mixed in the diet and the fifth group (G5) , served as control group . Results:
The worst histopathological changes are in G3 . A highly significant decrease in the mean cell lysate level of p21was found in different studied groups(G1,G2&G3) especially in(G3) where P values in these groups when compared to control group were (P < 0.0001) . In curcumin treated group(G4), there were downregulation in the mean cell lysate level of p21 gene when compared to control group but higher than other studied groups (G1,G2&G3) . A highly significant increase in the mean serum level of Bcl-2 was found in different studied groups(G1,G2&G3) especially in(G3) where P values in these groups (G1,G2,G3) when compared to control group were (P < 0.0001). (G4) showed higher Bcl-2 serum level when compared to control group, this difference was insignificant but this level is lower than other studied groups (G1,G2&G3) . Conclusion:
The results in the present study indicate that both P21 and Bcl-2 can be used as biological markers in the diagnosis of bladder cancer . Curcumin have the ability to overcome the decrease in p21WAF1/CIP1 protein and the increase of Bcl-2 protein and reduce the induction of carcinogenic effect .
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