AbdElhamid, H., Settin, A., El-baz, R., Elnaby, S., Roshdy, S., Noaman, A. (2015). Genetic Polymorphisms of Vascular Endothelial Growth Factor (VEGF) in Egyptian Women with Breast Cancer. The Egyptian Journal of Hospital Medicine, 60(1), 291-302. doi: 10.12816/0013788
Hamdy AbdElhamid; Ahmed Settin; Risk El-baz; Sobhy Hassab Elnaby; Sameh Roshdy; Ahmed Noaman. "Genetic Polymorphisms of Vascular Endothelial Growth Factor (VEGF) in Egyptian Women with Breast Cancer". The Egyptian Journal of Hospital Medicine, 60, 1, 2015, 291-302. doi: 10.12816/0013788
AbdElhamid, H., Settin, A., El-baz, R., Elnaby, S., Roshdy, S., Noaman, A. (2015). 'Genetic Polymorphisms of Vascular Endothelial Growth Factor (VEGF) in Egyptian Women with Breast Cancer', The Egyptian Journal of Hospital Medicine, 60(1), pp. 291-302. doi: 10.12816/0013788
AbdElhamid, H., Settin, A., El-baz, R., Elnaby, S., Roshdy, S., Noaman, A. Genetic Polymorphisms of Vascular Endothelial Growth Factor (VEGF) in Egyptian Women with Breast Cancer. The Egyptian Journal of Hospital Medicine, 2015; 60(1): 291-302. doi: 10.12816/0013788
Genetic Polymorphisms of Vascular Endothelial Growth Factor (VEGF) in Egyptian Women with Breast Cancer
1(Department of physiology , Faculty of science , Mansoura University , Mansoura , Egypt)
2(Department of Genetics , Faculty of Medicine , Children Hospital Mansoura University , Mansoura , Egypt),
3(Department of Genetics , Faculty of Medicine , Children Hospital Mansoura University , Mansoura , Egypt)
4(Department of Cytogenetic& Molecular Biology, Faculty of science, Menoufiya University ,Menoufiya,Egypt),
5(Department of General Surgery & Oncology , Faculty of Medicine , Mansoura University , Mansoura , Egypt)
6(Department of physiology , Faculty of science , Mansoura University , Mansoura , Egypt),
Abstract
Background:Vascular endothelial growth factor (VEGF) was considered to have an association with breast cancer because it regulates endothelial cell proliferation, migration and differentiation. Subjects and methods: One hundred and fifty two women with breast cancer were compared to 100 healthy control Egyptian women recruited from the same locality. VEGF gene polymorphisms were assessed using the PCR-RFLP analysis of DNA samples obtained from peripheral blood.SNP scanning was performed using MnII , BsmfI , CviAII , BsmfI , MnII restriction enzymes for VEGF1154 G/A, 634 G/C, 405 C/G, 936 C/T, 1612 G/A polymorphisms, respectively. Results : Breast cancer among Egyptian women was strongly associated with the mutations related to VEGF gene polymorphism as follows: VEGF 1154 G allele frequency was significantly higher than the A allele (P = 0.0007,O.R =2.4) , VEGF 634 C allele frequency was significantly higher than the G allele (P = 0.012, O.R =0.62),VEGF 405 C Allele frequency was significantly higher than G Allele (P = 0.009, O.R =1.67), VEGF 936 C Allele frequency was significantly higher than the T Allele (P = 0.0057, O.R =1.72), VEGF 1612 G Allele frequency was significantly higher than A allele (P = 0.0148, O.R =1.62). For VEGF 1154 GA: AA vs. GA+GG (Recessive) P = 0.10, O.R = 6.23, C.I (1.0-38.9), GA vs. AA+GG (over dominant) P= 0.01*, O.R = 2.13, C.I (1.2-3.8),AA+GA vs. GG (dominant) P= 0.0015*, O.R = 2.57, C.I (1.5-4.5). For VEGF 634 GC : CC vs. GC+GG (Recessive) P= 0.1852, O.R = 0.64, C.I (0.4-1.2), GC vs. CC+GG (over dominant) P= 0.2669 , O.R = 0.71, C.I (0.4-1.2), CC+GC vs. GG (dominant) P = 0.0002**, O.R=0.05, C.I (0.0-0.2).For VEGF 405 CG : GG vs. CG+CC (Recessive) P= 0.0013*, O.R = NA,C.I =NA, CG vs. GG+CC (over dominant) P= 0.877, O.R = 1.08, (0.6-1.9), GG+CG vs. CC (dominant) P = 0.0323*, O.R=1.93,C.I (1.1-3.4).For VEGF 936 CT : TT vs. CT+CC (Recessive) P = 0.1833, O.R = 1.63, C.I (0.9-3.1), CT vs. TT+CC (over dominant) P = 0.1379, O.R = 1.55, C.I (0.9-2.6), TT+CT vs. CC(dominant) P = 0.0075**, O.R=2.08, C.I (1.2-3.5). For VEGF 1612 GA: AA vs. GA+GG (Recessive) P = 0.0000**, O.R = NA, C.I = NA, GA vs. AA+GG (over dominant) P= 0.0002**, O.R = 0.36, C.I (0.6-0.2), AA+GA vs. GG (dominant) P = 0.9541, O.R = 0.95, C.I (1.6-0.6).
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