Histological and Immunohistochemical Study on the Effect of Bisphenol A on the Retina of Female Albino Rat

B N, Hassan; A S, Alazzouni; N A, Al Jalaud; M E, Hassan

doi:10.12816/0018761

Histological and Immunohistochemical Study on the Effect of Bisphenol A on the Retina of Female Albino Rat

Document Type : Original Article

Authors

¹ Department of Zoology, Faculty of Science, Helwan University

² Department of Biology, Faculty of Science, Dammam University, Saudi Arabia

³ Department of Ophthalmology, Faculty of Medicine, Al Azhar University

10.12816/0018761

Abstract

Background: Bisphenol A (BPA) is a well-known endocrine disruptor used to manufacture polycarbonate plastics and epoxy resins. Exposure of rats to low doses of BPA results in histopathological effects in their retina.
Objectives: We used histological and immunohistlogical techniques for determining retina pathological changes in response to low doses of BPA and the modulating effect of both vitamin A and stem enhancer.
Methods: Twenty female albino rats orally administered with 20mg BPA /kgb.wt/day for 45 days and then divided to groups and treated with vitamin A and stem enhancer. Both eyes were examined histologically and immunohistochemicaly to determine the histopathological changes in retinal layers.
Results: A remarkable degenerative histopathological changes in the ganglion cell layer and inner nuclear layer appeared with bis phenol treated rats and a clear improvement was seen after treatment with both vitamin A and stem cell enhancer.
Conclusions: Both of vitamin A and stem enhancer have ameliorative effect on the degenerative changes in the retinal layers and damaged estrogen receptors by the action of bisphenol A.

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