Microrna 192 Gene Expression in Type II Diabetic Nephropathy

El-Monem, Atef A.; Mahfouz, Mohamed H.; Mohamed, Mona A.; Abd El-Aziz, Heba Gamal; Hussien, Nora

doi:10.12816/0038187

Microrna 192 Gene Expression in Type II Diabetic Nephropathy

Document Type : Original Article

Authors

¹ National institute of diabetes and endocrinology (NIDE)

² Biochemistry Department, Faculty of science (Girls), Al-Azhar University

³ Biochemistry Departments, Faculty of Pharmacy (Girls), Al-Azhar University

10.12816/0038187

Abstract

Background: Diabetic nephropathy (DN) is the common cause of kidney failure in patients with diabetes mellitus. MicroRNAs (miRNAs) are short non-coding RNAs of about 22 nucleotides which recently have been shown to play vital roles in mammalian gene expression. Aim of the study: was to investigate the role of miRNA-192 in the pathogenesis of diabetic nephropathy and disease progression. Patients and Method: Sixty five patients with uncontrolled diabetes mellitus, they were subdivided into; thirty nine patients with normoalbuminuria (<20mg/L); their ages ranged between 48-67 years and the onset of disease between 1-5 years; twenty six patients with microalbuminuria (20-200 mg/L), their ages ranged between 47-66 years and the onset of disease between 5-15 years, in addition to twelve apparently healthy individuals as control; their ages ranged between 51-67 years. Serum Transforming growth factor beta (TGF-β), Interleukin 18 (IL-18) were determined using ELISA technique, the expression level of miRNA-192 in whole blood using (RT-PCR) was determined, other biochemical parameters as fasting plasma glucose (FPG), glycated haemoglobin (HbA1c), lipid profile and creatinine were estimated using commercial available kits. Patients were given written contest. Results: The level of miRNA-192 expressions was significantly lower in microalbuminuria group when compared to normoalbuminuria group. Serum level of IL-18 and TGF-β were significantly higher in both patient groups when compared to control group and their levels were significantly higher in microalbuminuria group than normoalbuminuria group. Conclusion: Together with TGF-β1 and IL-18, miRNA- 192 may not only be used as molecular biomarker in diabetic microvascular complications but also as early marker of alterations in specific biological processes in the kidney.

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