Evaluation of The Diagnostic Role of Non-Coding RNA and Exosomal Related Gene Association in Lung Cancer

Marwa Ali1 And Ghada Mohamed1, Fawzia Khalil1, Hanan H. Shehata1, Nehad Osman2, Omar Abdel-Rahman3,

doi:10.21608/ejhm.2018.9131

Evaluation of The Diagnostic Role of Non-Coding RNA and Exosomal Related Gene Association in Lung Cancer

Document Type : Original Article

Author

Fawzia Khalil1, Hanan H. Shehata1, Nehad Osman2, Omar Abdel-Rahman3, Marwa Ali1 and Ghada Mohamed1

1 Medical Biochemistry and Molecular Biology Department, 2 Pulmonary Medicine Department, Clinical Oncology and Nuclear Medicine Department, Faculty of Medicine –Ain Shams University

10.21608/ejhm.2018.9131

Abstract

Background: Exosomes are microvesicles that are recently discovered in intercellular communication especially between tumor cells as lung cancer that are important for tumor development and progression. Objectives: Examine the diagnostic role of serum exosomal LncRNA in lung cancer among Egyptian population.
Methods: Lung cancer characteristic exosomal RNA-based biomarker Lnc-RNA-RP11-510M2.10 was selected based on bioinformatic methods, followed by RT-qPCR validation of their expression in serum of 20 patients with lung cancer and 10 healthy volunteers.
Results: serum exosomal Lnc-RNA-RP11-510M2.10 showed a significant negative association with lung cancer patients in comparison to patients with healthy persons (p<0.001).
Conclusion: Lnc-RNA-RP11-510M2.10 could be used as diagnostic and prognostic biomarker tools for lung cancer.

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