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Rayea, M., Amer, M., Mousa, A., Farghaly, M. (2020). The Effect of Two Different Sperm Cryopreservation Methods on The Integrity of Its DNA Content (a Comparative Study). The Egyptian Journal of Hospital Medicine, 78(1), 21-27. doi: 10.21608/ejhm.2020.66842
Mohammed M Farid Rayea; Medhat Kamel Amer; Ahmed Abdelhady Mousa; Mohammed Gamal Farghaly. "The Effect of Two Different Sperm Cryopreservation Methods on The Integrity of Its DNA Content (a Comparative Study)". The Egyptian Journal of Hospital Medicine, 78, 1, 2020, 21-27. doi: 10.21608/ejhm.2020.66842
Rayea, M., Amer, M., Mousa, A., Farghaly, M. (2020). 'The Effect of Two Different Sperm Cryopreservation Methods on The Integrity of Its DNA Content (a Comparative Study)', The Egyptian Journal of Hospital Medicine, 78(1), pp. 21-27. doi: 10.21608/ejhm.2020.66842
Rayea, M., Amer, M., Mousa, A., Farghaly, M. The Effect of Two Different Sperm Cryopreservation Methods on The Integrity of Its DNA Content (a Comparative Study). The Egyptian Journal of Hospital Medicine, 2020; 78(1): 21-27. doi: 10.21608/ejhm.2020.66842

The Effect of Two Different Sperm Cryopreservation Methods on The Integrity of Its DNA Content (a Comparative Study)

Article 3, Volume 78, Issue 1, January 2020, Page 21-27  XML PDF (577.67 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2020.66842
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Authors
Mohammed M Farid Rayea1; Medhat Kamel Amer1; Ahmed Abdelhady Mousa2; Mohammed Gamal Farghaly1
1Department of Andrology, Sexology and STDs, Faculty of Medicine, Cairo University
2Department of Andrology, Sexology and STDs, Faculty of Medicine,Bani Suef University
Abstract
Background: Sperm cryopreservation is currently the only clinically available technique for preservation of male gametes for subsequent use in assisted reproduction technologies. Objective: The objective of this study was to determine the impact of semen cryopreservation on human sperm DNA. Patients and methods: The study sample consists of 20 male patients seeking seminal fluid analysis at the andrology laboratory of a specialized IVF center (ADAM International Hospital for Fertility and Sterility, Giza, Egypt), and accepting to sign a consent of approval to participate in the study, with the diagnoses of normozoospermia, oligozoospermia (either isolated or combined with asthenozoospermia or teratozoospermia). Results: There is no statistical difference between Pre-freezing and vitrification regarding DNA fragmentation, P>0.05. No statistical difference between Pre-freezing and slow frozen regarding DNA fragmentation, P>0.05. Finally, there is no significant statistical difference between vitrification and slow frozen regarding DNA fragmentation, P>0.05. Conclusion: The sperm DNA fragmentation index was not affected during cryopreservation under the various methods of storage tested. Clinicians and investigators should take this information into consideration when using cryopreserved sperm for assisted reproduction.
Keywords
Sperm cryopreservation; DNA content
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