Role of SERPIN A5 in Fertilizing Potential of Human Spermatozoa

Background: Infertility affects 12.5% of couples all over the world, with paternal factors accounting for about 50% of infertility cases. It was assumed that SERPIN A5 could be used as a seminal biomarker for evaluation of semen quality in varicocele-related infertility.
Aim: This study aimed to identify the role of SERPIN A5 in fertilizing potential of human spermatozoa.
Methods: This case control study was conducted on 30 cases astheno-teratozoospermia, 30 cases were oligo-astheno-teratozoospermia (OAT) versus 30 age-matched control group. Level of SERPINA5 protein in sperm pellet was measured. Alpha glucosidase was measured. Determination of malondialdehyde (MDA) was conducted.
Results: A significant difference was detected as regards halo, halo percent and acrosin index between control and astheno-teratozoospermia group and between control & oligo-astheno-teratozoospermia and also between astheno-teratozoospermia versus oligo-astheno-teratozoospermia. A significant difference was recorded between studied groups regarding Serpin A5 and DNA fragmentation between control and astheno-teratozoospermia group and between control & oligo-astheno-teratozoospermia and also between astheno-teratozoospermia versus oligo-astheno-teratozoospermia. Higher median serpin A5 was detected among control group followed by astheno-teratozoospermia then oligo-astheno-teratozoospermia. Lower median DNA fragment was detected among control group followed by astheno-teratozoospermia then oligo-astheno-teratozoospermia. Lower mean FSH was detected among control followed by astheno-teratozoospermia and the highest for oligo-astheno-teratozoospermia.
Conclusion: Serpin A5 played an important role in the context of fertilizing potential of human spermatozoa. It significantly increased among healthy subjects compared to oligo-astheno-teratozoospermia.