Ameliorating Effect of 10-Dehydrogingerdione against Cerebral Cortex Damage caused by Tramadol in Adult Male Albino Rats (Histological and Immunohistochemical study)

Document Type : Original Article

Abstract

Background: An artificial centrally acting analgesic drug called tramadol hydrochloride is used to treat moderate to severe pain with fewer side effects than conventional opioid medicines.
Objective:This study aimed to investigate effects of tramadol on rat cerebral cortex, focusing on biochemical, histological, and immunohistochemical alterations, and possible protective effect of ginger.
Materials and methods: Fifty-five albino rats were employed. They were split into four groups: Control group (15 rats) that was further split into three equal subgroups: One received saline, one received ginger oil (200 mg/kg B.W/day), and one was kept without any medicine. Tramadol group (15 rats) received tramadol (50 mg/kg B.W/day). 3rd group received ginger oil 30 minutes before tramadol intake. In order to examine effects of stopping tramadol, 15 rats in 4th group were given tramadol for 4 weeks before being released from therapy for a further 4 weeks. Samples and specimens were prepared for biochemical and microscopic examination. Results: Tramadol administration revealed a histological and biochemical abnormality, where there was loss of normal arrangement of cortical layers with distorted-shape cells. In addition, there was appearance of vacuolations around them and in neuropil. In 2nd group, there was an increase in MDA level and a decrease in SOD and GPx. Ginger and tramadol-treated group showed proof of remarkable enhancement in histology of tissue and level of oxidative markers in contrast to tramadol group, while withdrawal group demonstrated partial recovery.
Conclusion: Ginger oil ameliorated tramadol-induced damage in rat’s cerebral cortex.

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The Egyptian Journal of Hospital Medicine
Volume 97, Issue 1
October 2024
Pages 4003-4013

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