Ibrahim, H., Khorshid, S., Mohamed, T., Sayed, D., Hosny, T. (2023). Study of Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascetic Patients by Using 16S Ribosomal RNA Gene PCR. The Egyptian Journal of Hospital Medicine, 90(2), 3723-3729. doi: 10.21608/ejhm.2023.293425
Hoda Abdeen Ibrahim; Soha Esmat Khorshid; Tahia Mohamed Ahmed Mohamed; Dalia Hani Mohamed Sayed; Thoraya Hosny. "Study of Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascetic Patients by Using 16S Ribosomal RNA Gene PCR". The Egyptian Journal of Hospital Medicine, 90, 2, 2023, 3723-3729. doi: 10.21608/ejhm.2023.293425
Ibrahim, H., Khorshid, S., Mohamed, T., Sayed, D., Hosny, T. (2023). 'Study of Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascetic Patients by Using 16S Ribosomal RNA Gene PCR', The Egyptian Journal of Hospital Medicine, 90(2), pp. 3723-3729. doi: 10.21608/ejhm.2023.293425
Ibrahim, H., Khorshid, S., Mohamed, T., Sayed, D., Hosny, T. Study of Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascetic Patients by Using 16S Ribosomal RNA Gene PCR. The Egyptian Journal of Hospital Medicine, 2023; 90(2): 3723-3729. doi: 10.21608/ejhm.2023.293425
Study of Rapid Diagnosis of Spontaneous Bacterial Peritonitis in Cirrhotic Ascetic Patients by Using 16S Ribosomal RNA Gene PCR
Background: In cirrhosis, spontaneous bacterial peritonitis )SBP) is the most frequent infection . Rapid and precise identification of bacteria in clinical and scientific settings has been greatly aided by the 16S rRNA gene. Objective: Assessment of role of 16S rRNA gene in diagnosing SBP among cirrhotic ascitic cases. Patients and methods: our study was done on 60 adults cirrhotic ascitic patients, classified to 2 groups: Group I (SBP group ═ 38): involved all cases with ascitic fluid PMNL ≥ 250 cells/mm3, Group II (Non SBP group ═ 22): involved all cases with ascitic fluid PMNL < 250 cells/mm3. Ascitic fluid (AF) examination, bacterial culture and polymerase chain reaction (PCR) for detection of DNA were assessed among all cases. Results: Significantly greater levels of CRP were seen in the SBP group in comparison to the non-SBP group. Culture had sensitivity 53.3%, specificity 68.2%, PPV 70.5%, NPV 64.9 % and accuracy 60% for SBP diagnosis. PCR had sensitivity 94.7%, specificity 63.3%, PPV 81.8%, NPV 87.5% and accuracy 83.33% for SBP diagnosis Conclusion: Rapid and precise identification of AF infection is crucial for successful therapy, and polymerase chain reaction (PCR) detection of the 16s rRNA gene in ascitic fluid demonstrates this.