Detection of Bacterial Isolates Associated with Semen Among Persons with Temporary Infertility in the City of Ramadi

Document Type : Original Article

Authors

biology microbiology Cultivation of microorganisms

Abstract

Background: Infertility is the inability to bear children after at least one year of unprotected intercourse. It is a health problem that affects about 10% of the world's population.  
Aim of the work: This study aims to examine semen-associated bacterial isolates of people with temporary infertility and to determine the appropriate antibiotic.
Materials and Methods: Samples were collected from patients after they had abstained from sexual activity for 3-7 days before semen sample collection. Positive bacterial isolates were isolated for bacterial culture and growth on the surface of blood agar, MacConkey agar, or cooked blood agar and diagnosed according to standard and biochemical tests. The final diagnosis of bacterial isolates was performed using detection strips for Gram-positive and Gram-negative APIE20.
Results: The study showed that most isolates of Gram-positive bacteria were Staphylococcus aureus (25.97%), followed by Streptococcus agalactiae (18.93%). The study showed that Escherichia coli was the most prevalent among Gram-negative isolates (31.6%), followed by Klebsiella pneumoniae (8.97%), followed by Pseudomonas aeruginosa (3.39%). The study also showed that all bacterial isolates are sensitive to the antibiotics Levofloxacin, Meropenum, Doxycycline, and Amikacin, respectively.
Conclusion: The presence of bacterial isolates in the semen is a clear reason for temporary sterility and the need to treat them with antibiotics.
 

Keywords

The Egyptian Journal of Hospital Medicine
Volume 90, Issue 2
January 2023
Pages 3446-3453

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