The Level of Survivin in The Blood and Urine Samples of Bladder Cancer Patients Using the ELISA Method

Ali, Aesha S.; Ali, Mohammed R.; Hachim, Sadeq K.

doi:10.21608/ejhm.2022.277589

The Level of Survivin in The Blood and Urine Samples of Bladder Cancer Patients Using the ELISA Method

Document Type : Original Article

Authors

¹ Department of Microbiology, College of Medicine, Kirkuk University, Kirkuk, Iraq

² Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad, Iraq.

³ Department of Nursing, Institute of Medical Technology/Baghdad, Middle Technical University, Baghdad, Iraq

10.21608/ejhm.2022.277589

Abstract

Background: Survivin is a member of inhibitors of the apoptosis family (IAP), it is over-expressed in almost all cancers including bladder cancers in which it is excreted in urine.
Objectives: to find the possible suitability of survivin in urine and blood as a novel prognostic and or predictive molecular marker in bladder carcinoma.
Patients with methods: urine and serum samples were taken from five groups: healthy persons, patients who had non-neoplastic urinary tract problems, genitourinary cancers excluding bladder cancer, patients with discovered newly or recurrent superficial urinary bladder carcinoma and patients with muscle-invasive or metastatic bladder cancer. All were attendants of the Urology department at Al-Kadhimiya Teaching Hospital in Baghdad. Survivin levels were analyzed by ELISA test in urine and serum samples of the five groups
Results: The level of survivin in the normal control group was below the cutoff value in serum (71.385 pg/ml) and cutoff value in urine (71.86 pg/ml) while in non-neoplastic urological conditions the level of the survivin was evaluated above the cutoff value in serum and urine in 1/6 of the cases. While it was evaluated in half of the patients with urological malignancies other than bladder cancer and it was increased above the cutoff volume in 73.68% in the serum of patients with superficial urinary carcinoma and 78.9% in the urine of the same group.
Conclusions: specific and sensitive determination of urine and (or) serum survivin provides a simple, non-invasive diagnostic method to complement cytology and (or) other diagnostic markers in persons with new onset or recurrent urinary bladder carcinoma.

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