Molecular Detection of Plasmid-Mediated AmpC in Gram Negative Bacteria Isolated from Intensive Care Unit Patients in Wasit Province, Iraq

Document Type : Original Article

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Abstract

Background: The intensive care unit (ICU) plays a crucial role in managing and treating some of the most complex and serious disorders that affect the human body.
Patients and Methods: A total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted to the ICU. in this study admitted to the intensive care units (ICUs) of Alzahraa and Alkarama hospitals for more than 48 hours between 3rd  October 2021 to 20th February 2022. Each patient's name, age, gender, underlying clinical condition, ICU admission date, indoor admission date, previous antibiotic intake history, current ICU therapy, and clinical outcome were recorded. ‎
Results: Thirty (36.5%) female and‏‎ 52 (63.5%) male patients were admitted to the ICU. They were distributed 69 (84.1%) gram-‎negative bacteria. Gram positive 13 (15.9%). Nitrofurantion ‎was most active against E. coli. Piperacillin-‎tazobactam was most active against ‎Proteus mirabilis. AmpC ß-lactamase was detected by phenotypic and genotypic ‎procedures, phenotypically AmpC producers for klebsiella pneumoniae ‎‎13 (68%). Genotypically isolates blaEBC 4 (30.7%), blaCIT ‎‎13 (53.8%). However, phenotypically survey of AmpC pseudomonas aeruginosa was ‎‎9 (45%). Genotypically isolates blaEBC was 6 (50%). Phenotypically survey of AmpC producers for E. coli was 9 (52%) and ‎genotypically isolates blaEBC 3 (30%), blaCIT 1 (10%). While, DHA, ‎MOX ACC, FOX genes were absent among all isolates. Conclusion: klebsiella pneumoniae showedAmpC ß-lactamase comprised(blaEBC andblaCIT. while no isolate have DHA, MOX ACC, FOX genes). As for E.Coli AmpC ß- lactamase comprised (blaEBC andblaCIT, while no isolates have AmpC DHA, MOX ACC and FOX.
 

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The Egyptian Journal of Hospital Medicine
Volume 89, Issue 2
October 2022
Pages 6488-6495

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