Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

Document Type : Original Article

Authors

Abstract

Background: Fructophilic lactic acid bacteria (FLAB) are a group of LAB with unique growth features and are regarded as potential bioactive compound manufacturers. FLAB is discovered in D- fructose rich niches that favors D-fructose as a growth substrate triumphs over D-glucose. On D-glucose, they require electron acceptors to proliferate. The organisms have similar metabolic processes for carbohydrates. Fructobacillus spp < /em>. are Lactobacillus kunkeei, are members of this unique group.
Methodology: Inhibitory effect of filtrate from four FLAB isolates from honeybee was detected against the growth of pathogenic Klebsiella pneumoniae isolates. Biofilm formation, and the expression of fimH, mrkD, and NDM-1, and OXA-1 virulence genes were also tested.
Results: It was found that FLAB has antibacterial and antibiofilm effect. The incubation of K. pneumoniae with FLAB suspension at different three-time intervals (3, 6, and 9 hrs.) decreased expression of fimH, mrkD, NDM-1, and OXA-1virulence genes compared with the expression of the same genes in the absence of FLAB.
Conclusion: all FLAB filtrate isolates tested, K. pneumoniae fimH, mrkD, NDM-1, and OXA-1virulence gene expression was down-regulated, when compared to the control culture.
 

Keywords

The Egyptian Journal of Hospital Medicine
Volume 89, Issue 2
October 2022
Pages 6438-6444

Files

Share

How to cite

Statistics