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Hasan, H., Jasim, H., Salih, G. (2022). Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples. The Egyptian Journal of Hospital Medicine, 89(2), 6356-6360. doi: 10.21608/ejhm.2022.269974
Hussam Mahmood Hasan; Hameed M. Jasim; Ghada Mohammed Salih. "Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples". The Egyptian Journal of Hospital Medicine, 89, 2, 2022, 6356-6360. doi: 10.21608/ejhm.2022.269974
Hasan, H., Jasim, H., Salih, G. (2022). 'Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples', The Egyptian Journal of Hospital Medicine, 89(2), pp. 6356-6360. doi: 10.21608/ejhm.2022.269974
Hasan, H., Jasim, H., Salih, G. Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples. The Egyptian Journal of Hospital Medicine, 2022; 89(2): 6356-6360. doi: 10.21608/ejhm.2022.269974

Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples

Article 47, Volume 89, Issue 2, October 2022, Page 6356-6360  XML PDF (584.17 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2022.269974
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Authors
Hussam Mahmood Hasan email ; Hameed M. Jasim; Ghada Mohammed Salih
Abstract
Background: Klebsiella pneumonia is a ubiquitous encapsulated bacterial pathogen which cause various types of infections, this ability comes from the resistance gene and virulence factor genes.
Methodology: Fifty clinical isolates of Klebsiella pneumoniae was diagnosed and characterized according to their chemical and molecular characteristics, then genomic DNA was extracted from each bacterial isolate to detect carbapenem-resistant (NDM-1, and OXA-1), and biofilm association gene including (fimH, and mrkD) by amplification of these genes using specific primers.
Results: Results showed that NDM-1 and OXA-1 were found to be frequent in bacterial isolates in a percentage of 54% and 28% respectively. On the other hand, it was found that fimH is the most frequent in bacterial isolates (100%), while mrkD was found in a percentage of 86% of the total isolates.
Conclusion: High prevalence of biofilm association genes and presence of carbapenem-resistant in varied isolates of K. pneumoniae pose an important public health thing.
 
Keywords
Klebsiella pneumoniae; Antibiotic resistance genes; Virulence genes
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