Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples

Hasan, Hussam Mahmood; Jasim, Hameed M.; Salih, Ghada Mohammed

doi:10.21608/ejhm.2022.269974

Detection of Carbapenem-Resistant Genes and Specific Biofilm Association Genes in K. Pneumoniae Isolated from Medical Samples

Document Type : Original Article

Authors

10.21608/ejhm.2022.269974

Abstract

Background: Klebsiella pneumonia is a ubiquitous encapsulated bacterial pathogen which cause various types of infections, this ability comes from the resistance gene and virulence factor genes.
Methodology: Fifty clinical isolates of Klebsiella pneumoniae was diagnosed and characterized according to their chemical and molecular characteristics, then genomic DNA was extracted from each bacterial isolate to detect carbapenem-resistant (NDM-1, and OXA-1), and biofilm association gene including (fimH, and mrkD) by amplification of these genes using specific primers.
Results: Results showed that NDM-1 and OXA-1 were found to be frequent in bacterial isolates in a percentage of 54% and 28% respectively. On the other hand, it was found that fimH is the most frequent in bacterial isolates (100%), while mrkD was found in a percentage of 86% of the total isolates.
Conclusion: High prevalence of biofilm association genes and presence of carbapenem-resistant in varied isolates of K. pneumoniae pose an important public health thing.

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