Clinical Significance of Urine Podocyte Messenger Ribonucleic Acids in Idiopathic Nephrotic Syndrome and Lupus Nephritis Patients

Document Type : Original Article

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Abstract

Introduction:  Podocytes are specific epithelial cells that surround the outside of the glomeruli's basement membrane (GBM). They play a significant role in glomerular function. This study aimed to detect the presence of podocyte in idiopathic nephrotic syndrome and lupus nephritis patients, and to correlate them with the proteinuria levels.
Methods: Patients were divided to three groups (patients with idiopathic nephrotic syndrome, patients with lupus nephritis and, healthy controls. Quantification of podocyte mRNA proteins in urinary sediment by real time PCR was performed to all groups. Results: For those who had nephrotic syndrome showed significant increase in the alpha actin 4 value (p= 0.001) and significant decrease in the value of synaptopodin and podoclyxin in relation to controls (p= 0.001). The level of urinary expression of mRNA of podocytes correlated positively to renal function tests and estimated glomerular filtration rate between nephrotic and control groups. The reverse occurred with proteinuria. A significant rise in the alpha actin 4 value, podocin and synaptopodin in comparison with control (p= 0.001). Podocalyxin was decreased significantly (p=0.021). There was a statistically significant difference, when comparing renal function tests and estimated glomerular filtration rate between LN and control group. Also, an increase in the value of podocin in active group relative to control and non-active groups (p= 0.001 and p= 0.007, respectively).
Conclusion: Significant increase in level of mRNA of alpha actin in nephrotic syndrome, while mRNA podocin, synaptopodin and alpha actin were increased in LN, with podocin could mark increased activity of the disease.
 

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The Egyptian Journal of Hospital Medicine
Volume 89, Issue 1
October 2022
Pages 6016-6023

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