Abu Elmagd, H., Hassan, E., Sarhan, M., Abu-Elnag𝐚, N. (2022). A Modified ICSI Technique: Using Zona Pellucida as A Natural Bait. The Egyptian Journal of Hospital Medicine, 89(1), 5075-5082. doi: 10.21608/ejhm.2022.261173
Hager Ali Abu Elmagd; Eman Anwar Hassan; Moustafa Sarhan; Nehal Abu-Elnag𝐚. "A Modified ICSI Technique: Using Zona Pellucida as A Natural Bait". The Egyptian Journal of Hospital Medicine, 89, 1, 2022, 5075-5082. doi: 10.21608/ejhm.2022.261173
Abu Elmagd, H., Hassan, E., Sarhan, M., Abu-Elnag𝐚, N. (2022). 'A Modified ICSI Technique: Using Zona Pellucida as A Natural Bait', The Egyptian Journal of Hospital Medicine, 89(1), pp. 5075-5082. doi: 10.21608/ejhm.2022.261173
Abu Elmagd, H., Hassan, E., Sarhan, M., Abu-Elnag𝐚, N. A Modified ICSI Technique: Using Zona Pellucida as A Natural Bait. The Egyptian Journal of Hospital Medicine, 2022; 89(1): 5075-5082. doi: 10.21608/ejhm.2022.261173
A Modified ICSI Technique: Using Zona Pellucida as A Natural Bait
Background: In vivo, the zona pellucida (ZP) of the oocyte can bind to normally functional sperm. The ZP-sperm interaction is one of the final steps of natural selection during their journey in the female reproductive tract. Aim: In the current study, we evaluated the ability of the ZP of immature oocytes to harvest the fittest sperm. Method: We compared the embryological outcomes (i.e., rates of fertilization, cleavage, blastocyst formation, and high-quality blastocysts) of intracytoplasmic sperm injection (ICSI) using conventionally selected sperm (control group) and ZP-bound sperm (treated group). Results: Our results showed that there were no statistically significant differences for the ZP binding technique over the conventional sperm selection with respect to the rates of fertilization and cleavage. However; the rates of blastocyst formation and high-quality blastocysts were significantly higher in the treated group compared to the control group. Conclusion: These findings imply that the proposed technique (ZP-bound sperm) can serve as a cost-effective and natural sperm selection method that has the potential to improve the embryological and clinical outcomes of intracytoplasmic sperm injection (ICSI).