The Potential Histological Effect of Experimental Obesity on The Liver of Male Albino Rats (Light and Electron Microscopic Study)

Document Type : Original Article

Author

Faculty of Medicine - Al-Azhar University - Assiut - Egypt

Abstract

Background: There is a growing global awareness to the hazards of obesity. The obese patients are much more liable to many serious diseases including liver failure.
The aim of work: was to study light and electron microscopic changes of the liver, as well as, the liver enzymes in the experimentally obese albino rats.
Methodology: Sixty male albino rats were divided to 3 groups: 1- control group < /strong>: was fed standard laboratory diet for 4 weeks. 2- Obese group < /strong>: were given one intraperitoneal injection of Triton WR 1339 to induce obesity and fed normal laboratory diet for 4 weeks. 3- Recovery group < /strong>: similar to obese group but left for additional 4 weeks for recovery. The weights of animals were recorded, blood samples were collected for liver enzymes (ALT, AST and GGT) and the livers were weighted. Liver samples were fixed in glutaraldehyde for EM examination; and other samples were fixed in 10% formol saline then stained by H&E, Mallory trichrome and PAS stains for LM examination.
 Results: Obese animals had significantly higher body weights, liver weights, and liver enzymes than the control group. Histologically, there was a non-significant increase in the collagen area of obese group. There was a highly significant reduction in the optical density of glycogen in the obese group. However, the optical density in the recovery group was lower than that of control group. Conclusion: obesity may induce several abnormal changes in the hepatocytes, and inflammation similar to steatosis or steatohepatitis.
 

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The Egyptian Journal of Hospital Medicine
Volume 89, Issue 1
October 2022
Pages 5028-5039

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