Gabr, K., Shaheen, A., Abd-Elmouttaleb, A., Ibrahim, A. (2019). The Role of Nuclear Factor Kappa B Rs28362491 Single Nucleotide Gene Polymorphism in Recurrent Embryo Implantation Failure. The Egyptian Journal of Hospital Medicine, 74(4), 949-956. doi: 10.21608/ejhm.2019.25565
Khalid Tahseen Ahmed Gabr; Abd EL-Mohsen Mousa Shaheen; Ashraf Taha Abd-Elmouttaleb; Adel Elsayed Ibrahim. "The Role of Nuclear Factor Kappa B Rs28362491 Single Nucleotide Gene Polymorphism in Recurrent Embryo Implantation Failure". The Egyptian Journal of Hospital Medicine, 74, 4, 2019, 949-956. doi: 10.21608/ejhm.2019.25565
Gabr, K., Shaheen, A., Abd-Elmouttaleb, A., Ibrahim, A. (2019). 'The Role of Nuclear Factor Kappa B Rs28362491 Single Nucleotide Gene Polymorphism in Recurrent Embryo Implantation Failure', The Egyptian Journal of Hospital Medicine, 74(4), pp. 949-956. doi: 10.21608/ejhm.2019.25565
Gabr, K., Shaheen, A., Abd-Elmouttaleb, A., Ibrahim, A. The Role of Nuclear Factor Kappa B Rs28362491 Single Nucleotide Gene Polymorphism in Recurrent Embryo Implantation Failure. The Egyptian Journal of Hospital Medicine, 2019; 74(4): 949-956. doi: 10.21608/ejhm.2019.25565
The Role of Nuclear Factor Kappa B Rs28362491 Single Nucleotide Gene Polymorphism in Recurrent Embryo Implantation Failure
1Department of Medical Biochemistry, International Islamic Center for Population Studies and Research, Faculty of Medicine, Al-Azhar University
2Department of Assisted Reproductive Unit, International Islamic Center for Population Studies and Research, Faculty of Medicine, Al-Azhar University
Abstract
Background: Embryo implantation is a very intricate process, which is controlled by a number of complex molecules like hormones, cytokines, growth factors and their cross talk at feto-maternal interface. The transcription factor nuclear factor kappa B (NF-κB) induces the expression of various pro-inflammatory genes, including those encoding cytokines and chemokines, and also participates in inflammasome regulation. Aim of the present study:was to investigate the possible associations between NF-κB rs28362491 single nucleotide gene polymorphism and susceptibility to repeated implantation failure (RIF). Subjects and Methods: This study was conducted on 120 subjects subdivided into 2 groups: 60 women with successful implantation asacontrol group (Group I), and 60 womendiagnosed withRIF(Group II). They were subjected to full history taking, clinical examination and investigated for molecular testing of the NF-κB -94 ins/del ATTG (rs28362491) polymorphism by lymphocyte separation and extraction of DNA for polymerase chain reaction (PCR) together with determination of serum concentration of day 3 follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol II (E2), prolactin and thyroid stimulating hormone (TSH) concentrations by an automated quantitative enzyme-linked fluorescent immunoassay (ELFA). Results: A significant difference in the genotype distribution of the NF-κB rs28362491 between those with RIF and controls. Specifically, subjects with RIF had a significantly higher frequency of the NF-κB rs28362491 del allele than that of controls. Conclusion: This study showed a significant association between rs28362491 in the promoter region of the NF-κB gene and RIF. NF-κB appears to be a triggering factor of orchestrated gene regulation during embryo implantation.
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