Osteoprotegerin, Soluble Receptor Activator Nuclear Factor-κB Ligand, Nuclear Factor Kappa B and Intestinal Trefoil Factor 3 are Promising Biomarkers in Diagnosis and Follow- up Inflammatory Bowel Disease Patients

Salama, Ragaa Hamdy; Medhat, Mohammed A; Elghazally, Shimaa A; Farag, Nesma G; El Sanory, Aya A; Herdan, Mohamed O; El Sanory, Tasneem  A; Kamel, Amira  A.

doi:10.21608/ejhm.2022.212851

Osteoprotegerin, Soluble Receptor Activator Nuclear Factor-κB Ligand, Nuclear Factor Kappa B and Intestinal Trefoil Factor 3 are Promising Biomarkers in Diagnosis and Follow- up Inflammatory Bowel Disease Patients

Document Type : Original Article

Authors

Assiut University, Faculty of Medicine, Medical Biochemistry Department

10.21608/ejhm.2022.212851

Abstract

Background: Inflammatory bowel disease (IBD) is a condition in which the gastrointestinal system becomes inflamed for no apparent reason. Its incidence increased during the last few decades. As a result, it's critical to understand how to validate a clinical diagnosis and monitor any progress using a simple procedure. Osteoprotegerin (OPG), which interacts with RANKL, regulates RANK/RANKL signaling. OPG is a decoy receptor for soluble receptor activator of nuclear factor-B ligand (sRANKL), which stimulates nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and the healing trefoil factor 3 (TFF3), which could be employed as in the diagnosis and follow-up of IBD patients, biomarkers are used. Objectives: The aim of the current work was to evaluate the role of RANKL (sRANKL), OPG, NF-κB , and TFF3 in in the diagnosis of active IBD. Subjects and methods: Thirty-five newly diagnosed untreated IBD patients and thirty-five healthy controls were included in this study. Patients were then given particular treatment either in the form of cortisone, mesalamin, immunotherapy or infliximab and immunotherapy for 3 months and attend for second visit. OPG, sRANKL, NF-κB and TFF3 levels were estimated by ELISA method using commercial kits in patients before and after treatment and healthy control. Results: Patients with IBD had significantly increased levels of OPG, sRANKL, NF-B, and TFF3 than healthy controls (P =0.0001). After 3 months of treatment, patients' levels of NF-B and TFF3 were significantly lower (p = 0.0001 and 0.03, respectively). Conclusion: The biomarkers OPG, sRANKL, NF-κB , and TFF3 show promise in the diagnosis of active IBD. The decrease in NF-κB and TFF3 following treatment suggests that be used as biomarkers to track disease activity and treatment effectiveness.

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