Histopathological And Histochemical Studies On The Effect Of Taurine In Preventing Carbon Tetrachloride –Induced Hepatic Injury In The Albino Rat

Hassan, Nabila S.; Abbas, Naglaa F.; Sharaf, Hafiza A.

doi:10.21608/ejhm.2003.18728

Histopathological And Histochemical Studies On The Effect Of Taurine In Preventing Carbon Tetrachloride –Induced Hepatic Injury In The Albino Rat

Document Type : Original Article

Authors

Pathology Department– National Research Center

10.21608/ejhm.2003.18728

Abstract

Taurine is an amino acid– like compound, it is found mostly in meat and fish.This study was designed to evaluate the effects of carbon tetrachloride (CCL4) on liver Histopathological & Histochemical changes and the protective role of taurine (2aminoethanosulfonic acid ) was studied . Fourty two albino rats were divided into seven groups : control, taurine alone (200mg/kg), CCL4 alone, CCL4 plus 50mg/kg of taurine, CCL4 plus 100mg/kg of taurine, CCL4 plus 200mg/kg of taurine (taurine was injected twice daily for one week before CCL4 treatment), CCL4 plus 200mg/kg of taurine with and after CCL4 treatment. The sections (six microns) of rat liver were stained with haematoxyline and eosin for histological examination. Total protein content, alkaline phosphatase (Alk.ph), succinic dehydrogenase (SDH) ,and lipids were demonstrated.CCL4 treatment showed vacuolar degeneration , necrosis , mononuclear cellular infiltration around the central vein and fatty degeneration .These histological changes which appeared in the animals treated with CCL4 alone were more extensive and severe than those seen in the animals treated with CCL4 plus taurine . The incidences of total protein content, and SDH reaction were markedly reduced in CCL4 treated rats than in those protected with taurine . The Alk.ph. activity and lipid content were markedly higher in the CCL4 treated rats than in those protected with taurine.Taurine in this study ,not only reduced the hepatocellular damage but also improved the hepatocellular functions.

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