Effect Of Diazepam On The Prenatal Development Of Neuropil Of The Purkinje Cell Layer Of The Cerebellar Cortex Of The Rats Embryo (Electron Microscope Study)

Document Type : Original Article

Authors

1 Histology Department Faculty of Medicine, Al-Azhar University

2 Anatomy Department Faculty of Medicine, Al-Azhar University

3 Anatomy Department Faculty of Medicine, Al-Azhar University (Girls)

Abstract

Diazepam is one of the most widely used sedative hypnotic agents, and many women take this drug during pregnancy.
The purpose of this study was to investigate the effects of diazepam on the development of the neuropil of the Purkinje cell layer of the cerebellar cortex.
Twenty female pregnant rats were used in this study. The rats were divided into two groups, the first group (5 rats) was used as control group and the second group (15 rats) were given 10 mg/kg/day of diazepam orally and sacrificed at 15th, 20th, 25th days of pregnancy. It was difficult to differentiate between the dendrites and the axonal knobs at 15th day of pregnancy. As age advanced, the dendrites were swollen with absence of cytoplasmic organelles or contained few free ribosomes and immature mitochondria. The observed axonal knobs contained ill-defined synaptic vesicles. Immature axo-dendritic synapses were recognized on the 15th and 20th days of pregnancy, while on the 25th days of pregnancy symmetrical axo-dendritic synapses were recognized. From this study, it appears that the exposure to diazepam causes marked changes on prenatal development of neuropil of the Purkinje cell layer of the cerebellar cortex.
Aim of the work
The aim of the work was to study the effects of diazepam on the development of the neuropil of Purkinje cell layer of the cerebellar cortex.

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