Farghaly, L., Sabak, N., El-sherbeny, N. (2007). The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats. The Egyptian Journal of Hospital Medicine, 26(1), 31-45. doi: 10.21608/ejhm.2007.17778
Lamia M. Farghaly; Nagwan A. Sabak; Naglaa A. El-sherbeny. "The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats". The Egyptian Journal of Hospital Medicine, 26, 1, 2007, 31-45. doi: 10.21608/ejhm.2007.17778
Farghaly, L., Sabak, N., El-sherbeny, N. (2007). 'The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats', The Egyptian Journal of Hospital Medicine, 26(1), pp. 31-45. doi: 10.21608/ejhm.2007.17778
Farghaly, L., Sabak, N., El-sherbeny, N. The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats. The Egyptian Journal of Hospital Medicine, 2007; 26(1): 31-45. doi: 10.21608/ejhm.2007.17778
The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats
1Departments of Histology Faculty of Medicine, Suez Canal University
2Biochemistry Faculty of Medicine, Suez Canal University.
3Pharmacology, Faculty of Medicine, Suez Canal University.
Abstract
Aim of the study: This study was conducted to investigate the possible protective effects of L- treptophan "a precursor of melatonin" and alpha lipoic acid against L- arginine-induced experimental acute pancreatitis in albino rats. Methods: Fourty adult male albino rats (200- 250g) were randomized into 4 groups (n= 10). Group I, the control group was given 0.9% saline intraperitoneally (i.p). Group II, was given 500 mg/100g L-arginine (i.p) as a single dose to induce acute pancreatitis. Group III: was given 250mg/kg L-tryptophan (i.p) 30 min prior to L- arginine injection. Group IV: was given 50mg/kg alpha lipioc acid(i.p) 30 min prior to L-arginine. Before scarifice, blood samples were obtained from all groups to assay serum amylase and interleukin 6. Animals were sacrificed after 6 hours. For the histopathological study, pancreatic tissue was prepared for histological (H&E, PAS) histochemical ( Tween stain for lipases) and immunohistochemical ( Bax stain for apoptosis) techniques. Both qualitative and quantitative analyses were done to assess the degree of acinar cells affection Results: It was revealed that serum amylase and interleukin 6 in group II rose rapidly. Microscopically, severe acinar cells degeneration, interstitial edema, diffuse bleeding and inflammatory infiltration were demonstrated. These changes were markedly improved with the administration of both L- tryptophan and alpha lipoic acid. Conclusion: It was concluded that both L- tryptophan & alpha lipoic acid reduced the effects of L-arginine-induced acute pancreatitis with better protection achieved by L-tryptophan administration.
View on SCiNiTO
Top