Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.

N, Hanafi; El Aaser, Abel Baset; Gabry, Mohamed S.; Tohamy, Amany A; Ebrahim, Manal El Saied

doi:10.21608/ejhm.2007.17777

Effect of increase in Antioxidants Supplementation on Kidney Tissue of Mice Subjected to Fractionated Dose of γ-Irradiation.

Document Type : Original Article

Authors

¹ Radiation Biology Department, National Centre for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt

² cancer Biology Depart. Cancer Institute, Cairo University

³ Zoology Dept. Faculty of Science, Helwan University.

10.21608/ejhm.2007.17777

Abstract

Introduction: Although there is little doubt that antioxidants are a necessary component for good health, no one knows if supplements should be taken and, if so, how much.
Materials and methods: To investigate the increase in antioxidants supplementation, a group of mice feeding on a diet containing 20% soybean was used as control group. The control group treated with 5% Nigella sativa and Antox drug (3 mg/mouse/day) either alone or in combination for 15 days. Another control group was treated with the same antioxidants and subjected to 1 Gy of γ- irradiation x 5 times day after day. In kidney tissue the levels of malonaldehyde (MDA) and reduced glutathione (GSH) were estimated. Also, the histopathological changes were recorded.
Results: 5% Nigella sativa revealed a little decrease in MDA level and a highly significant decrease in GSH in kidney tissue while the exposure to fractionated dose of γ- irradiation predicted a highly significant decrease in MDA and a highly significant increase in GSH level. Gastric intubations of Antox drug showed a highly significant increase in MDA and GSH levels in kidney tissue either alone or in addition to fractionated radiation exposure. Combined treatments of Nigella sativa and Antox drug showed a highly significant decrease in MDA level and a highly significant increase in GSH level in kidney tissue in comparison to the control group. Histopathological changes showed that treatment with 5% Nigella sativa and Antox drug either alone or in combination revealed toxic effect on kidney tissue. However, their treatments prior to radiation exposure showed ameliorating effect.

Conclusion: Our findings indicated that we under oxidative stress.