Kandil, A., F, R., D, T., M, H., K, S. (2009). Comparative Study on the Effects of Phenyl Boron and Boric Acid on the Development and Gene Expression (Connexin X43 and E-Cadherin) of Pre- implantationBovine Embryos. The Egyptian Journal of Hospital Medicine, 35(1), 364-377. doi: 10.21608/ejhm.2009.17586
Asmaa M Kandil; Rings F; Tesfaye D; Hölker M; Schellander K. "Comparative Study on the Effects of Phenyl Boron and Boric Acid on the Development and Gene Expression (Connexin X43 and E-Cadherin) of Pre- implantationBovine Embryos". The Egyptian Journal of Hospital Medicine, 35, 1, 2009, 364-377. doi: 10.21608/ejhm.2009.17586
Kandil, A., F, R., D, T., M, H., K, S. (2009). 'Comparative Study on the Effects of Phenyl Boron and Boric Acid on the Development and Gene Expression (Connexin X43 and E-Cadherin) of Pre- implantationBovine Embryos', The Egyptian Journal of Hospital Medicine, 35(1), pp. 364-377. doi: 10.21608/ejhm.2009.17586
Kandil, A., F, R., D, T., M, H., K, S. Comparative Study on the Effects of Phenyl Boron and Boric Acid on the Development and Gene Expression (Connexin X43 and E-Cadherin) of Pre- implantationBovine Embryos. The Egyptian Journal of Hospital Medicine, 2009; 35(1): 364-377. doi: 10.21608/ejhm.2009.17586
Comparative Study on the Effects of Phenyl Boron and Boric Acid on the Development and Gene Expression (Connexin X43 and E-Cadherin) of Pre- implantationBovine Embryos
1Department of pharmacology National Organization for Drug Control and Research.
2Institute of Animal Science, Animal Breeding and Husbandry group Bonn University, Germany.
Abstract
The different actions of Boron (as Phenyl boron (PB) or Boric acid (BA)) on the growth of pre- implantation embryos and their (Connexin X43(CX43) and E-Cadherin) gene expression was investigated. In the first and two experiments, phenyl boron or boric acid was added as 0, 20 and 250 μM/L in embryo culture medium at 18-20 h after in-vitro fertilization (IVF). The percentage of development to blastocyst stage was assessed from day 7 to day 9 post treatment. The experiment was repeated two times using 50 fertilized zygotes /treatment. The total of two of 10 blastocysts pools from each treatment group was used for mRNA isolation for quantification of Cx43 and E-cadherin gene mRNA, in the embryos of each treatment group was assessed by real time quantitative PCR. The results indicated that the rate of development to blastocyst is higher in blastocysts group treated with PB than that of BA. The highest development rate was at the 20μM/L PB and the lowest was at the 20μM/L BA treatment. The development of zygotes treated with 250μM/L BA was arressted at 4cell stage then they degenerated. Relative expression levels of CX 43 were very high at doses of 250μM/L PB and 20 μM/L BA while at the same doses the relative expression levels of E-cadherin were very low.
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