ANTIOXIDANT AND PROTECTIVE EFFECT OF AROMATIC PLANTS BLEND INFUSION AGAINST OXIDATIVE STRESS OF STREPTOZOTOCINE AND CARBON TETRACHLORIDE IN RATS

El-massry, Khaled F.; El-Ghorab, Ahmed H.; Ramadan, Manal M.; Gad, Ahmed M.

doi:10.21608/ejhm.2009.17579

ANTIOXIDANT AND PROTECTIVE EFFECT OF AROMATIC PLANTS BLEND INFUSION AGAINST OXIDATIVE STRESS OF STREPTOZOTOCINE AND CARBON TETRACHLORIDE IN RATS

Authors

Flavour and Aroma Department - National Research Center, Dokki, Cairo, Egypt

10.21608/ejhm.2009.17579

Abstract

Aim of the work: The present study aimed to use some aromatic plants as ingredients to prepare a healthy drink with an acceptable flavour and taste and to evaluate its chemoprotective activity against oxidative stress induced by streptozotocin (STZ) and carbon tetrachloride (CCl₄) in rats. Materials and Methods: Infusion of guava leaves, linden, ginger, corn silk and chamomile was prepared, its volatiles were isolated and analyzed using GC and GC/MS, sensory evaluation as well as the total phenolic content were determined. The chemoprotective effectiveness of the blend infusion was tested by two separate biological experiments against the oxidative stress of STZ and CCl₄. Serum glucose level was determine as well as liver function, kidney function, lipid profile, plasma hemoglobin, antioxidant enzymes and malonaldehyde (MDA) in some organs were investigated. Results: Thirty nine compounds were identified in the volatiles of blend infusion. The main components were 1,8-cineol (35.97%), cumene (7.12%), guryunene (5.25%), b- patchoulene (4.55%), citronellol (2.97%) and a- zingiberene (1.76%) and these compounds are related to the characteristic volatiles of the different aromatic plants that constitute the blend infusion. The blend infusion exhibits high score for all sensory attributes
The results showed that the supplementation with blend infusion to intoxicated rats either with STZ or CCL₄ with blend infusion for four weeks significantly ameliorate most of the toxic effects and protecting pancreas, kidney and liver through improving their antioxidant status. In conclusion: The aromatic plants blend infusion was safe and effective in controlling hyperglycemic effects of STZ and improve lipid metabolism as well as its hepatoprotective activity against CCl₄by amelioration of the associated biochemical parameters.

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