Elkerdasy, H., Elshazly, A., Baioumy, B., El Sayed, Y., Hussein, A. (2021). The Possible Protective Effect of Melatonin and Coenzyme Q10 on Lung Injury Induced by Bleomycin in Adult Male Albino Rats. The Egyptian Journal of Hospital Medicine, 83(1), 1536-1543. doi: 10.21608/ejhm.2021.171400
Hanan Elkerdasy; Amal Mahmoud ElSafy Elshazly; Bodour Qassim BadrEldeen Baioumy; Yasmeen Mohammed Ismail El Sayed; Asmaa Y.A. Hussein. "The Possible Protective Effect of Melatonin and Coenzyme Q10 on Lung Injury Induced by Bleomycin in Adult Male Albino Rats". The Egyptian Journal of Hospital Medicine, 83, 1, 2021, 1536-1543. doi: 10.21608/ejhm.2021.171400
Elkerdasy, H., Elshazly, A., Baioumy, B., El Sayed, Y., Hussein, A. (2021). 'The Possible Protective Effect of Melatonin and Coenzyme Q10 on Lung Injury Induced by Bleomycin in Adult Male Albino Rats', The Egyptian Journal of Hospital Medicine, 83(1), pp. 1536-1543. doi: 10.21608/ejhm.2021.171400
Elkerdasy, H., Elshazly, A., Baioumy, B., El Sayed, Y., Hussein, A. The Possible Protective Effect of Melatonin and Coenzyme Q10 on Lung Injury Induced by Bleomycin in Adult Male Albino Rats. The Egyptian Journal of Hospital Medicine, 2021; 83(1): 1536-1543. doi: 10.21608/ejhm.2021.171400
The Possible Protective Effect of Melatonin and Coenzyme Q10 on Lung Injury Induced by Bleomycin in Adult Male Albino Rats
1Department of Anatomy and Embryology Faculty of Medicine, Benha University
2Department of Clinical Pharmacology, Faculty of Medicine, Benha University
3Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University
Abstract
Background: bleomycin-induced lung toxicity and oxidative damage by decreasing the deactivating enzyme, genetic vulnerability, and released cytokines of inflammation. Melatonin has a free radical detoxifying effect, coenzyme Q10 has a strong antioxidant effect. Aim of the study: this study aimed to evaluate the possible protective role of melatonin and coenzyme Q10 in bleomycin-induced lung injury in Albino rats. Material and Methods: forty male Albino rats were categorized into five groups; group I (control group), group II (bleomycin group): rats were given a single dose of bleomycin intra-tracheal for inducing lung injury, group III (melatonin group): rats were given melatonin for three weeks after intratracheal installation of bleomycin, group IV (coenzymeQ10 group): rats were given coenzymeQ10 for three weeks after intratracheal installation of bleomycin and group V (combined melatonin and Co Q10 group): rats were given a combination of melatonin and coenzyme Q10for three weeks after induction of bleomycin lung toxicity. Lung tissues were prepared for biochemical, histological and immunohistochemical studies. Results: bleomycin produced a significant increase in the level of malondialdehyde and a significant reduction in glutathione peroxidase activity in lung tissues with loss of normal histological lung architecture, significant elevation in main area percent of collagen fibers deposition and caspase-3 immuno positive expression. In group III melatonin enhanced a significant improvement in the biochemical changes, moderate prevention of histopathological changes in lung tissue with a significant reduction in main area percent of collagen fibers deposition and caspase-3 immuno positive expression. While, in group IV co enzyme Q10 enhanced non significant improvement in the biochemical changes, mild prevention of histopathological changes and non-significant reduction in main area percent of collagen fibers deposition and caspase-3 immuno positive expression. Using a combination of both drugs in group V enhanced a significant improvement in the biochemical changes and almost preservation of normal histological architecture of the lung tissue. Conclusion: administration of both melatonin and coenzyme Q10 produced almost a complete recovery of bleomycin induced lung injury.