Comparative studies on the effect of some antioxidants on renal dysfunction in rats

Fadel, Hewaida A.E.; Hassanin, Essayed A.; Rihan, Zakaria E.; Abd Allah, Eveleen S.

doi:10.21608/ejhm.2012.16364

Comparative studies on the effect of some antioxidants on renal dysfunction in rats

Document Type : Original Article

Authors

¹ Department of Nutritional Chemistry& Metabolism, National Nutrition `Institute

² Department of Endocrinology, Military Medical academy

³ Department of Home Economies, Ain Shams Univerersity

10.21608/ejhm.2012.16364

Abstract

The present study is carrying out for investigating the effect of supplementation with some vitamins (A, E&C) as natural antioxidant extracts in renal dysfunction in rates.

Material& Methods: 40 adult male Sprague – Dawley rats (150 – 200 g) divided to two groups. First group: 8 rats were fed on standard diet (S.D.), as a control group. Second group: 32 rats were injected intraperitonial with a single dose of Cis-diammine dichloride Platinum II (CDDP) for inducing renal dysfunction (2.5 mg/Kg) then it was divided to six subgroups each one contained 8 rats. (1): fed on (S.D.) nephrotoxic group. (2): fed on (S.D.) + Vitamin A (15 mg/Kg body weight/day). (3): fed on (S.D.) + Vitamin E (317 I.U. /kg body weight/day). (4): fed on (S.D.) + Vitamin C (280 I.U. /kg body weight/day). The experimental period was four weeks, results were statistically analyzed.

Results: The results proved that groups of nephrotoxicity rats supplemented with Vitamin A,E and C showed significant increase in food intakes, body weight gain and food efficiency ratio (FER) (28.9%, 29.4% and 19.2%), (870.5%, 1615.6% and 409.8%) and (652.5%, 122502% and 327.3%) respectively, compared with nephrotoxic group. The nephrotoxicity rats supplemented with Vitamin A and showed significant reduction in serum vitamin E &kidney glutathione content (9.3% & 47.9%), while nephrotoxicity rats supplemented with Vitamin E&C showed significant increase in serum vitamin E &kidney glutathione content (27.9% & 116.6%), (13.7%&55.8%)respectively. The results showed that nephrotoxicity rats supplemented with Vitamin A,E and C showed significant reduction in serum urea nitrogen and creatinine (48.01% and 55.2%), (52.6% and 60.3%) and (57.0% and 63.04%) respectively. Best results in histopathological examination of kidney were in vitamin A and vitamin C groups.

Conclusion: These results suggest that natural antioxidants could be beneficial as additional therapy in renal dysfunction.

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