Histpathological and Immunohistochemical Changes Induced by Contraceptive Pills in the Cervix of Female Rabbits

MS, Gabri; MA, Ibrahim; DH, Abd El-kader; BN, Hassan

doi:10.12816/0018489

Histpathological and Immunohistochemical Changes Induced by Contraceptive Pills in the Cervix of Female Rabbits

Document Type : Original Article

Authors

¹ Department of Zoology and Entomology, Faculty of Science, Helwan University.

² Department of Zoology and Entomology, Faculty of Science, Helwan University

³ Department of Histology, Faculty of Medicine, Cairo University

10.12816/0018489

Abstract

Introduction: oral contraceptive pills (OCPs) are the most popular form of hormonal contraception in young women.
Aim of the work: the present study focused on evaluating the effects of two different contraceptive pills including combined pills (estrogen and progesterone) and mini pills (progesterone only) on the cervix of female rabbit.
Material and methods: after three months of daily oral administration of these contraceptive pills the animals were sacrificed. The excised organs were dissected, processed and stained with H & E, PAS reaction and Masson’s trichrome stain and orcein stain. This was followed by morphometric measurements and statistical study. Results: this study revealed that contraceptive pills administration - specially the combined one - caused marked alterations in the form of hyperplastic cervical mucosal cells and hypertrophied muscular layer. Also, there was a significant increase in collagenous and elastic fibres content in the muscular layer of the cervix. There was also a statistically significant increase in PAS positive materials in the lumina of the mucosal glands of the cervix. All these changes were less marked in the case of mini pill treatment
Conclusion: pills of progesterone only showed marked histopathological change, in the cervix as a contraceptive mean, but in less manner than the combined pills (estrogen and progesterone).

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