Protective Effect of Olive Oil Against Teratogenecity Induced by Mancozeb in Albino Rats

A.A, Nahas; Abbas, Enas A.

doi:10.12816/0017694

Protective Effect of Olive Oil Against Teratogenecity Induced by Mancozeb in Albino Rats

Document Type : Original Article

Authors

Nahas A.A ¹
Enas A. Abbas ²

¹ Mammalian and Aquatic Toxicology Dep., Central Agricultural of Pesticides Lab. (CAPL), Agricultural Research Center

² Biochemistry Dept., Animal Health Research Institute, Agricultural Research Center

10.12816/0017694

Abstract

Aim: this study aimed to investigate the protective effect of extra-virgin olive oil (EVOO) against teratogenicity of the fungicide mancozeb. Methods: after pregnancy confirmation, 32 pregnant rats were divided into 4-groups (n=8). The 1^st group orally administered tap water (-ve control), the 2^nd group (+ve control) was administered EVOO (0.5ml/dam) from the1^st to 20^th day of pregnancy. The 3^rd and the 4^th groups were administered 200 mg/kg mancozeb during the period of organogenesis, from the 6^th to 15^th day of pregnancy. The 4^th group received the mentioned dose of EVOO prior to the pesticide administration. Cesarean section was performed on day 20 of pregnancy and the maternal and fetal parameters were recorded. Results: mancozeb induced maternal toxicity manifested as lower body weight gain of dams, increased number of late resorption sites/litter in comparison with the control group and mancozeb group pretreated with EVOO. Mancozeb evoked a decrease in fetal body weight, altered sex ratio (M/F) as well as increased incidence of fetal external, visceral and skeletal abnormalities. Treatment with virgin oil reduced the congenital malformations.
Conclusively, the present study elucidates the protective role of EVOO as a result of antioxidant activity which scavenges the reactive oxygen species which induced cytotoxicity and increased prenatal mortalities.

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