Objectives: We aimed to assess the effect of anemia on subjective sleep quality in patients with iron deficiency anemia (IDA). Methods: The present study is a prospective and cross-sectional study which has been carried out at King Abdulaziz hospital between November 2016 and April 2017. Fifty-two patients diagnosed with iron deficiency anemia and 40 healthy individuals, who are gender and age matched, were involved in the study. All participants were requested to fill 3 forms: a socio-demographic form (age, gender, marital status, income level and educational status), hospital anxiety and depression (HAD) scale and Pittsburgh sleep quality index (PSQI). Results: According to the HAD scale, the average anxiety score was found 9.31±2.41 in patients and 7.62± 2.11 in controls. And, the average depression score was 7.49±2.08 in patients and 6.39±2.73 in controls. The total sleep quality score was 6.68±2.99 in patients and 4.27±1.71 in controls. There was a statistically significant difference in terms of anxiety, depression and sleep quality scores. Linear regression analysis showed no association between anxiety and depression with poor sleeping. Conclusion: IDA affects sleep quality irrespective of psychological symptoms such as depression and anxiety.