Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Mitochondria and Nuclear changes in postimplanted mice embryos After treatment with Cisplatim antitumour drugs
1
11
EN
Hassan M.
El Ashmaoui
National Research center, Cell Biology Dept., Giza, Egypt
10.21608/ejhm.2004.18152
<span>To evaluate a possible relationship of maternal exposure to capsulation during postimp- lantation embryotoxicity, SWR pregnant female mice were injected intraperitoneally on day 4 of gestation with 7mg/kg cisplatin or with saline (0.4 ml) for the control group. Embryos were collected after 24h 48h, 72h, after injection. ultrastructural sections were used to investigate the mitochondria and the nuclear changes Both the mitochondria and the nuclei were damaged specially 48h and 72 h after injection they may be related to cytotoxicity of the drug.<br /> </span><span><br /></span>
platinum antitumour drugs,mitochondria,Nucleus,Mice embryos
https://ejhm.journals.ekb.eg/article_18152.html
https://ejhm.journals.ekb.eg/article_18152_4111122c27138ad2d5da531c48a39aea.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Reproductive and Developmental Effects of Moxifloxacin on Female Mice and Embryos
12
19
EN
Hanaa M.
Roshdy
Cell Biology Department, National Research Center Dokki, Cairo, Egypt
10.21608/ejhm.2004.18153
<span>Moxifloxacin (Avelox®) is a fluoroquinolone antibiotic with a broad spectrum of activity and bactericidal action. Moxifloxacin has </span><span>in vitro </span><span>activity against a wide range of Gram- positive and Gram-negative organisms. The safe use of moxifloxacin in human pregnancy has not been established. In order to evaluate the genotoxic and embryo toxic effects of (Avelox)® during pregnancy, Avelox was administrated orally to female mice with doses (8.7, 17 and 26 mg/kg/day) from 1 to 17 days of pregnancy. Caesarean sections were completed on gestation day 18 and complete fetal examinations and cytogenetic analysis were conducted. Decreases in the fetal body weights and increases in the external visceral and skeletal anomalies were found in all doses of (8.7, 17 and 26 mg) Avelox compared to the controls. Cytogenetic analysis in mothers and embryos revealed that all the tests doses produced chromosomal aberrations and micronuclei (MN) formations in a dose dependent manner compared to the controls. These results indicate that Avelox has a maternal and embryotoxic effects on the female mice and their embryos when administered with a recommended and above the recommended dose during pregnancy. </span>
<span> </span>
Moxifloxacin,chromosomal aberrations – micronuclei – mice – embryos
https://ejhm.journals.ekb.eg/article_18153.html
https://ejhm.journals.ekb.eg/article_18153_610e991f85b671794018a4b68c881c39.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Ultrastructural study of renal tubular damage induced by captopril in adult and fetal mice
20
43
EN
Hamdy H.
Swelim
Department of Zoology, Faculty of Science, Ain Shams University
Aleya A.
Sakr
Department of Zoology, Faculty of Science, Ain Shams University
10.21608/ejhm.2004.18154
<span>The present study has been designed to evaluate the possible nephrotoxicity of the angiotensin converting enzyme inhibitor, captopril on renal tubules of adult and maternally treated fetuses of CD-1 mice. The study included the effect of captopril administration for one month up to three months in adults, while in fetuses, they were exposed to the drug through their mothers in two periods. The first was from 6</span><span>th</span><span>-12</span><span>th </span><span>days of pregnancy, while the second was from 6</span><span>th </span><span>-18</span><span>th </span><span>day of pregnancy. The dose used in the present study represents the dose equivalent to the therapeutic daily dose taken by human. All the recorded tissue damage was found to be time dependent. The first remarkable feature noticed in all the treated adult animals was the presence of hyaline casts that obstructed most of the renal tubules. The second remarkable feature was the increase of the intertubular space associated with irregularity of the tubules due to the degeneration and vacuolation of the basal regions of the cells. Renal tubule cells showed large blebs, accumulaton of lipids, degeneration and necrosis. In maternally treated fetuses, the proximal convoluted tubule cells displayed moderate vacuolation and marked increase of lysosomes while some of the distal convoluted tubules revealed atrophy and their cells showed loss of mitochondria. In addition, the collecting tubules showed loss of microprojections. Worthy to mention that there was apparent increase of mesenchymal cells as well as fibroblasts in the fetuses maternally treated with captopril. The significance of these changes was discussed and it should be emphasized that captopril must be taken with caution for pregnant women and those who suffer from renal troubles. Moreover, kidney function should be monitored during therapy . </span>
https://ejhm.journals.ekb.eg/article_18154.html
https://ejhm.journals.ekb.eg/article_18154_4a9063199fd67efc4d12b21cce888f3d.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Chemical, Nutritional and Microbiological Evaluation of Some Egyptian Soft Cheeses
44
57
EN
Ghada, Z.
A. A.
Lecture of Biochemistry The National Nutrition Institute
Alia, M.
H
Lecture of Food Hygiene The National Nutrition Institute.
Soha, .
Al-S
Lecture of Nutrition Suez Canal University.
Magdy,
N. A.
Associate Prof of Ecological Sciences The National Nutrition Institute.
Mohammed,
F. S.,
Prof of Microbiology The National Nutrition Institute.
10.21608/ejhm.2004.18155
<span>Milk and dairy products is considered the most complete foodstuff that provide human either infants or adults with most of their vital needs. Milk and cheese have high nutritive value due to its high content of protein, fat, minerals especially calcium (Ca</span><span>2+</span><span>) & phosphorous, and vitamins. Two hundred samples produced and sold in Egypt during 2001-2003 were collected from allover the country. The cheese samples were subjected to microbiological and chemical analysis. Samples were microbiologically tested for total aerobic bacterial count (TABC), Colifrm, </span><span>Escherichia coli </span><span>(</span><span>E. coli)</span><span>, </span><span>Staphylococcus aureus</span><span>, mould and yeast, salmonella and shigella, and </span><span>listeria species</span><span>. Protein, fat, carbohydrates, moisture, ash, lactose, Calcium (Ca), phosphorous (P) and Ca/P were evaluated. The analysis showed that total aerobic bacterial count did not exceed 1.4X10</span><span>5</span><span>±1.7X10</span><span>5 </span><span>cells/gm, which is close to what allowed by the Standard Egyptian Guidelines (2001) and 47.5 % of the tested cheese are free from coliform bacteria and </span><span>Escherichia coli</span><span>. Ninety-eight and half percent, 97 %, 97 % and 91.5 % of the tested cheese (kareish, feta, thalag</span><span>a, double cream respectively), either made in plant or home or farmers’ </span><span>cheese sample have zero </span><span>Staphylococcus aureus </span><span>count or mould and yeast; or salmonella and shigella, or </span><span>listeria species </span><span>respectively, i. e. free from them. Double cream cheese has the lowest protein content (7.79±0.78 gm%) while kareish cheese has the highest protein content (19.99±1.32 gm%), but for fat content the opposite is true, double cream cheese have the highest fat content (24.56±1.78 gm%) while kareish cheese have the lowest fat content (3.87±0.97 gm %). Feta cheese has high ash content while kareish cheese has the highest moisture content with the lowest ash content (68.97±1.86 & 1.81±0.47 gm% respectively). Lactose content varies widely from 1.50±0.26 (double cream cheese) to 3.25±0.50 (feta cheese). Kareish cheese has higher content of calcium and phosphorous (641.1±49.21 mg%, 431.18±37.21 mg% respectively) than the remaining types of cheese. Calcium & phosphorous content of kareish cheese is almost the double content of the double cream cheese. Feta cheese has higher Ca/P (1.65±0.19) while thalaga and double cream has lower Ca/P (1.34±0.13 & 1.37±0.20). Each 100 gm of soft cheese can provide children (1-8 y) & adult (9-</span><span>≥50 y) from </span><span>39.78% & 24.48 % to128.22 % & 64.11% of their Ca Dietary Reference Intake and this from double cream cheese and kareish cheese respectively. </span>
https://ejhm.journals.ekb.eg/article_18155.html
https://ejhm.journals.ekb.eg/article_18155_8a11f67fdc86bc0ffda062aa86e93645.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Possible Association Between the Chemokine Receptor Gene CCR5- Delta32 Mutation and Hepatitis C Virus Pathogenesis
58
62
EN
Kouka Saad Eldin
Abdel-Wahab
Department of Medical Microbiology, Faculty of Medicine for Girls,
Mohamed
Foda
Department of Medical Virology School of Medicine, Kumamoto University, Japan;
Magda Abdel- Moneim
Gamil
Department of Medical Microbiology, Faculty of Medicine for Girls
Azza-Hassan
El-salakawy
Department of Medical Microbiology, Faculty of Medicine for Girls
Gamal
El-Attar
Department of Internal Medicine, Theodore Bilharz Institute, Imbaba, Egypt
Shinji
Harada
Department of Medical Virology School of Medicine, Kumamoto University, Japan
Yosuke
Maeda
Department of Medical Virology School of Medicine, Kumamoto University, Japan
10.21608/ejhm.2004.18157
<span>Background: </span><span>CCR5-Delta32, a 32-base pair deletion of the CC chemokine receptor (CCR)5 gene, is associated with slowed human immunodeficiency virus disease progression in heterozygotes and protection against infection in homozygotes between carriers and non-carriers of each genetic variant. The present study investigated the frequency and clinical consequence of the CCR%-Delta32 mutation in Egyptian HCV infected patients. Genomic DNA samples from 150 patients with chronic HCV infection were screened by PCR for the presence of the CCR5-Delta32 polymorphism. One hundred blood donors were used as control population. </span><span>Results: </span><span>The frequency of CCR5-Delta32 heterozygosity was 0.67% in chronic hepatitis C virus and 0% in controls. The CCR5-Delta32 allele was not associated with any of the clinical parameters of hepatitis C virus infection.<br /> </span><span>Conclusion: </span><span>In this study, the frequency of CCR5-Delta32 homozygosity in patients with hepatitis C was similar to controls. </span>
https://ejhm.journals.ekb.eg/article_18157.html
https://ejhm.journals.ekb.eg/article_18157_d1d3c7c86c049b893e6382b0703d6cba.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Cytogenetic and Developmental Effects of Antidepression Drug (Cipralex) on Female Mice and Embryos
63
69
EN
Hanaa M.
Roshdy
Cell Biology Department, National Research Center Dokki, Cairo, Egypt
Thanaa M.T
Shoman
Cell Biology Department, National Research Center Dokki, Cairo, Egypt
10.21608/ejhm.2004.18158
<span>Escitalopram (cipralex®) a new highly selective serotonin reuptake inhibitor, it is effective in the treatment of patients with major depression. To evaluate the cytogenetics and developmental effects of cipralex throughout major organgenesis, mice were administrated orally with a doses of 0.06, 0.12 and 0.24 mg/kg/day cipralex on gestation days 1-18 and examined on the 19</span><span>th </span><span>day of gestation for evidence of maternal and fetal toxicity. Cipralex at different doses tested produce significant toxic effects in reproductive parameters. Significant embryo fetotoxic effects were observed at tested dose levels as evidenced by total number of implantations, post. Implantation loss and embryo malformations. There were increases in the frequencies of micronuclei and chromosomal aberrations in both maternal and embryonic cells treated with cipalex, these increases were dose dependent. These results indicate that cipralex is considered to be cytogenetic and embryo toxic drug when administered during pregnancy. </span>
<span> </span>
Escitalopram,chromosomal aberrations – micronuclei – embryo – mice
https://ejhm.journals.ekb.eg/article_18158.html
https://ejhm.journals.ekb.eg/article_18158_5a38a496ea7c15b9cc370c748051fd6a.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
L -Carnitine and Melatonin Reverse CCl4 Induced Liver Fibrosis In Rats (Histological and Histochemical Studies)
70
92
EN
Fatma, A.
Morsy
Abdel Razik, H.
Farrag
Sonya, L.
El-Sharkawy
10.21608/ejhm.2004.18159
<span>Carbontetrachloride (CCl</span><span>4</span><span>) is closely related chemically to chloroform and likewise in hepatic poisons. This study was designed to evaluate the effects of carbon tetrachloride on liver of male rats and the reversing effects of L-carnitine and melatonin on established liver fibrosis. A total of 72 adult male albino rats were used in this study. The animals were divided into six groups. Group (1) animals of the first group were kept as control andtreated with paraffin oil twice weekly for eight weeks. Group (2) rats of the second group were injected with CCl</span><span>4 </span><span>intraperitoneally at 0.15 ml per rats (diluted 1:1 in liquid paraffin) twice weekly for eight weeks to produced liver fibrosis. Group (3) following establishment with CCl</span><span>4 </span><span>which induced liver fibrosis, the rats were treated with L-carnitine at a dose level of 50 mg/kg for four weeks. Group (4) rats with liver fibrosis were injected intraperitoneally with melatonin at dose level of 10 mg/kg for four weeks. The fifth and sixth groups were given L-carnitine and/or melatonin at dose levels of 50 mg/kg and 10 mg/kg respectively for four weeks. </span>
<span>Histological changes in the liver of rats treated with CCl</span><span>4 </span><span>including liver fibrosis, architecture distortion and appearance of many pseudolobule. The fibrous tissues run in septa between the nodules. The liver damage varied from one area to another and varied from moderate fibrosis to cirrhosis. </span>
<span>Quantitative measurement of the severity of liver fibrosis (area damage) was achieved by using computerized image analysis (Leica image) showed that highly significant increase in area of fibrosis was recorded in the case of rats treated with CCl</span><span>4 </span><span>only. </span>
<span>Quantitative DNA image analysis showed that 3% of aneuploid cells could be noticed in liver of rats treated with CCl</span><span>4 </span><span>only. Histochemical results of rats treated with CCl</span><span>4 </span><span>showed highly significant increase in grey level of mucopolysaccharides and protein levels. No histological and histochemical changes could be noticed in the liver of rats treated with either L- carnitine or melatonin only. Both L</span><span>–</span><span>carnitine and melatonin were found to reverse CCl</span><span>4 </span><span>induced liver damage.<br /> </span><span><br /></span>
L-carnitine,Melatonin,carbontetrachloride,Liver fibrosis,Rats,Histological,histochemical
https://ejhm.journals.ekb.eg/article_18159.html
https://ejhm.journals.ekb.eg/article_18159_da1d3e0480b76ce2607305efb6ac6156.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Biological effects of ivermectin on the fowl tick Argas (Persicargas) persicus (Oken) (Ixodoidea: Argasidae)
93
105
EN
Marzouk,
A.S
Department of Zoology, Faculty of Science, Ain Shams University
Swelim,
H.H
Department of Zoology, Faculty of Science, Ain Shams University
Montasser,
A.A
Department of Zoology, Faculty of Science, Ain Shams University
Gadallah,
A.I
Department of Medical Entomology, Faculty of Agriculture, Al-Azhar University
10.21608/ejhm.2004.18160
<span>The present study is carried out to assess the effect induced by different single subcutaneous injections of ivermectin (IVM) (100, 200, 400, 800 or 1600 </span><span>μ</span><span>g/kg pigeon weight) injected 2 or 7 days before tick feeding on some biological parameters such as mobility and viability, sexual activity, ingested blood, amount of coxal fluid, blood digestion and fertility in the tick </span><span>Argas (P.) persicus </span><span>to define the effective dose. This effective dose was used in similar assessment conducted 2 or 3 weeks post injection in order to confirm the degradation of ivermectin concentration in the host blood and to determine the number of required doses for complete control . From this study we conclude: 1) IVM induces complete immobilization of both males and females when they are fed on hosts injected by doses over 100 </span><span>μ</span><span>g/kg. 2) The use of two doses of 400 </span><span>μ</span><span>g/kg with a week interval completely controls the tick population. 3) Sexual response was completely negative at doses over 200 </span><span>μ</span><span>g/kg. 4) The amount of coxal fluid emitted by both sexes decreased markedly when fed after host injection with all doses, whereas the amount of ingested blood remained generally not highly affected. 5) The number of ovipositing females, number of eggs deposited and their hatching percent decreased markedly with the increase of dose used. Blood digestion was not noticed in males at doses >200 ug/kg and in females at doses >100ug/kg . </span>
Argas,Efficacy,Ivermectin
https://ejhm.journals.ekb.eg/article_18160.html
https://ejhm.journals.ekb.eg/article_18160_d40ccc12d582fffbbff9d187dda1abd8.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Acute Hepatitis E. virus infection in Egypt
106
114
EN
Osman
F
Abbassia Fever Hospital, Ministry of Public Health
Abu-Shady
EA
Microbiology Department, Faculty of Medicine – Al-Azhar University (Girls)
Abd El-Wahab
KSE
Microbiology Department, Faculty of Medicine – Al-Azhar University (Girls)
El-Rashidy
ZE
Microbiology Department, Faculty of Medicine – Al-Azhar University (Girls)
10.21608/ejhm.2004.18161
<span>Hepatitis E virus (HEV) is largely responsible for water borne epidemics in many developing countries. The principle mode of HEV transmission is the fecal oral route in epidemic and sporadic forms with a high case fatality ratio in pregnant women. Serum samples from 50 healthy subjects and from 435 acute viral hepatitis patients, 4-75 years old, were screened for markers of acute viral hepatitis. These included (HB</span><span>s</span><span>Ag, anti-HBc (IgM), anti- HDV (IgM), HAV (IgM), anti-HCV (IgG), and anti-HEV (IgG), and (IgM) tests by enzyme- linked immunoassays (EIA). </span>
<span>Furthermore isolation of HEV from peripheral blood lymphocytes and from stools belonging to anti-HEV IgG-positive patients was attempted by inoculation of HepG</span><span>2 </span><span>and Vero cell line cultures. The inoculated cell cultures were examined after immunoperoxidase staining for the detection of HEV antigen. Plasma, lymphocytes and stool samples from anti-HEV IgM positive patients were examined for HEV RNA by PCR.<br /> Anti-HEV IgG was found in 144/435 (33%) of these acute hepatitis patients. Anti-HEV (IgM) was detected in 8/52 (15.4%) out of 52 chosen from the 144 sera that were anti-HEV IgG positive cases. </span>
<span>HEV was isolated in HepG</span><span>2 </span><span>from 32.6% of lymphocyte and from 34.9% of stools from patients positive for anti-HEV (IgG). While it was isolated from 71.4% of lymphocytes and from 100% of stools from patients positive for anti-HEV (IgM). In Vero cell cultures there was no HEV isolation from stools but HEV was isolated from 50% of lymphocytes. HEV RNA was detected by PCR in 85.7% of stools, 62.5% of plasma, and in 37.5% of lymphocyte samples belonging to anti-HEV IgM positive cases. Analysis of these diagnostic tests indicated that virus isolation from peripheral blood lymphocytes and stools by inoculation of HepG</span><span>2 </span><span>cell cultures is more sensitive than virus-RNA detection by PCR </span>
https://ejhm.journals.ekb.eg/article_18161.html
https://ejhm.journals.ekb.eg/article_18161_8088a36a59ffaf74296fbc456fd5e896.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Ultrastructural Studies of The Pigment Epithelium of Retinae of Some Reptiles
115
129
EN
Fairoze
Khattab
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
Fahmy I.
Khattab
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
Nagui
Fares
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
Aman
Zaki
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
10.21608/ejhm.2004.18162
<span>The present work was designed to reveal the fine structure of the pigment epithelium of the retina in four different reptiles: the homed viper </span><span>Cerastes cerastes </span><span>(diurnal and nocturnal), the European </span><span>Chameleo chameleon </span><span>(diurnal),the gold skink </span><span>Eumeces schenrdii </span><span>(diurnal) and the Egyptian gecko </span><span>Traentola annularis </span><span>(nocturnal) . The pigment epithelium of each type reptiles possessed certain unique features in their morphology and ultrastructure to accommodate with their day and night activity. The most striking feature was the presence of myoid bodies in the pigment epithelium of the diurnal reptiles. These bodies trigger the photomechanical movement of the myoid region in cones of their retinae. </span>
https://ejhm.journals.ekb.eg/article_18162.html
https://ejhm.journals.ekb.eg/article_18162_401ea5c38abf17154b249259d248af63.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Immunohistochemical Study Of Bcl-2 Protein And Estrogen Receptor-Alpha Expression In Benign Prostatic Hyperplasia And Prostatic Carcinoma
130
142
EN
Ahmed H. Abel-Rahman- Ghada A.
Abdel-Aziz
Departments of Pathology, Dermatology & Veneriology Al-Azhar University (Assiut & Girls)
Ali Emad
S
Urology Al-Azhar University (Assiut & Girls)
Basset A.
Badawy
South Valley University
Alaa Ar.
Abdel-Hafez
South Valley University
10.21608/ejhm.2004.18163
<span>The human prostate, a male sexual accessory tissue involved in seminal fluid production, has a remarkably high incidence of hyperplastic and neoplastic disease. The present study was carried out on one hundred and twenty (120) specimens divided into two groups; group 1: Included forty cases of benign prostatic hyperplasia (BPH) and group 2: Included sixty cases of prostatic adenocarcinoma (PC) (22 were low grade; GS: 2-6 and 38 were high grade; GS: 7- 10),in addition to twenty cases of histologically normal prostates taken as controls. Immunohistochemical technique was applied to detect Bcl-2 as well as ER </span><span>α </span><span>positivity in all specimens. Group 1 showed the following profile: ER </span><span>α </span><span>(+) in all cases (100%), Bcl-2 (-) in 95%, ER</span><span>α </span><span>(+) / Bcl-2 (+) in 95%, ER</span><span>α </span><span>(-) / Bcl-2 (+) in 0%, ER</span><span>α </span><span>(+) / Bcl-2 (-) in 5% and ER</span><span>α </span><span>(-) / Bcl-2 (-) in 0% of cases while group 2 showed the following profile: ER</span><span>α </span><span>(+) in 30%, Bcl-2 (+) in 21.7%, ER</span><span>α </span><span>(+) / Bcl-2 (+) in 15%, ER</span><span>α </span><span>(-) / Bcl-2 (+) in 6.7%, ER</span><span>α </span><span>(+) / Bcl-2 (-) in 15% and ER</span><span>α </span><span>(-) / Bcl-2 (-) in 70% of cases. The mean epithelial ER </span><span>α </span><span>-immunolabeling was, however, significantly increased in group 2 than in group 1 (P < 0.05) which, in turn, being higher than the normal cases (P<0.05 ) . Among group 2 , the mean ER </span><span>α<br /> </span><span>was significantly more in high grade than in low grade tumors (P < 0.05), however, the mean ER </span><span>α </span><span>immunolabeling revealed no significant correlation with T-stage (P = 0.219) or with the clinical stage (P = 0.391). In contrast, the Bcl-2 immunostaining was statistically higher in group 1 than in group 2 (P < 0.05) and showed a significant correlation with T stage (P < 0.05) although the study displayed no significant correlation between Bcl-2 immunopositivity and either Gleason score (P = 0.125) or the histologic grade (P = 0.146). In addition, combined ER </span><span>α </span><span>(+)/ Bcl 2 (+) immunoreactivity demonstrated the aggressive subgroup of PC cases more accurately than either ER </span><span>α </span><span>(+) or Bcl-2 (+) alone. Finally, multivariate analysis showed that the Bcl-2, proved to be an independent prognostic indicator (P < 0.05). Thus, the immunohistochemical expression of ER </span><span>α </span><span>and Bcl-2 protein in prostatic tissue may aid in better understanding the biology and genesis of both prostatic hyperplasia and carcinoma . </span>
<span> </span>
Bcl-2,Estrogen receptor-alpha,Immunohistochemistry,Benign Prostatic Hyperplasia,prostatic carcinoma
https://ejhm.journals.ekb.eg/article_18163.html
https://ejhm.journals.ekb.eg/article_18163_c014df1f9aaf9e2bf1511f1f83071a38.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Mutagenic studies on the effect of Aldicarb “Temik” and vitamin C as antioxidant agent on the white rat: (Chromosomal aberrations and Micronucleus tests)
143
154
EN
Fatma M.
Hamam
Department Of Mammalian Toxicology, Central Agricultural Pesticides Laboratory
Ihab H.
Foda
Agricultural Research Center, Ministry Of Agriculture
10.21608/ejhm.2004.18164
<span>Widespread contamination of the environment due to increased and frequently indiscriminate usage of insecticides during the last two decades has aroused much concern over the possibility of their radiominetic effect. Evidence accumulating over the years emphasized the indisputable link between certain insecticides, chromosomal damage and possibility of gene mutation. There is a wide variety of insecticides, among which the carbamates. Their chemical relationship to ethyl carbamate makes them worthy of study for their possible deleterious effect on biological system. The main object of the present study is to evaluate the mutagenic effect of </span><span>a carbamate insecticide” Aldicarb” alone and in combination of vitamin C as an antioxidant </span><span>agent to decrease their mutagenicity. Male albino rats were tested orally for 48 hours , two doses of aldicarb were used in absence and in the presence of viamin C (1/4 and 1/10) LD50. The obtained data showed highly significant increase in the micronucleus (PCEM) and in chromosomal aberrations in rat bone marrow cells at the two doses of aldicarb compared to control group. (P< 0.0001). The frequency of chromosomal aberrations and micronucleus decreased in rats treated with aldicarb and vitamin C than in aldicarb treated group. From these results we concluded that cytogenetic effect of aldicarb might be decreased by the usage of vitamin as an antioxidant agent. </span>
<span> </span>
Carbamate,Aldicarb,Chromosomal aberration,micronucleus,Antioxidant agents
https://ejhm.journals.ekb.eg/article_18164.html
https://ejhm.journals.ekb.eg/article_18164_66e76188c63bf2c1e6c3a1bb4e31540e.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Evaluation Of Hydatid Disease (Echinoccosis) In Algmeil Hospital (2002 – 2003)
155
166
EN
Abdel-Hakim
Rezeeg
Assistant Professor Of Surgery AlgmeilHospital- Libia
10.21608/ejhm.2004.18165
<span>Hydatidosis is a zoonotic parasitic disease caused by the dog tapeworm </span><span>Echinococcus </span><span>and its larval stage, the hydatid cyst. Humans can accidentally become intermediate hosts by ingesting the eggs of the tapeworm. While most cysts deve lop in the liver and lungs. animals. At present, four species of the genus </span><span>Echinococcus </span><span>are recognized and regarded as taxonomically valid</span><span>: E. granulosus </span><span>(cystic hydatidosis), </span><span>E. multilocularis </span><span>(multivesicular hydatidosis), </span><span>E. vogeli </span><span>(polycystic hydatidosis) and </span><span>E. oligarthrus (Soulsby, 1982). </span><span>A total number of 23 patients were included in this study. 13 patients were females while the rest 10 were male patients. All cases were properly diagnosed as Hydated disease and then treated in the surgery Department of Algmeil Hospital (Libia) in the last 2 years (2002 and 2003). Proper investigations as well as treatment were carried out. The obtained results were statistically analyzed. Four types of presentation of the disease were observed in this study and presented, Asymptomatic 78.26%, Obstructive jaundice 8.69%, Accidental rupture 8.69% and Pressure symptom 4.34%. In spite of the progress in these areas, echinococcus/ hydatidosis remains a serious public health problem in a number of countries. It is very important to support and implement new control programmes so as to prevent further spread of the disease. Research in possible </span><span>vaccines </span><span>is essential in order to supplement the existing methods of breaking the Echinococcus life cycle. </span>
https://ejhm.journals.ekb.eg/article_18165.html
https://ejhm.journals.ekb.eg/article_18165_c2db642578d3c7a65a28be9fb6ae7b22.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Retinal Photoreceptor Fine Structure in some reptiles
167
186
EN
Fairoze
Khattab
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
Fahmy I.
Khattab
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
Nagui
Fares
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
Aman
Zaki
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
10.21608/ejhm.2004.18166
<span>The structure of the photoreceptors of four different reptiles: the homed viper </span><span>Cerastes cerastes </span><span>(diurnal and nocturnal), the European </span><span>Chameleo chameleon </span><span>(diurnal), the gold skink </span><span>Eumeces schneidrii </span><span>(diurnal) and the Egyptian gecko, </span><span>Tarentola annularis </span><span>(nocturnal) has been investigated by light and electron microscopy. </span>
<span>The photoreceptors of diurnal reptiles were mainly of the cone type and those of nocturnal were mainly rods. The ellipsoid region of both double rods in the nocturnals and large single cones in the species having both nocturnals and diurnal activity, consist of several mitochondria arranged in a remarkable radially gradient architecture which accommodates with the specific function of this region as a focusing device helping to condense light onto the outer segments. Moreover the principle cone of double cone and single cone of diurnal reptiles possessed a large oil droplet in the region between the inner segment and outer segment. This droplet is thought to play a role in filtering light and so doing enhanced contrast reduce glare and lessen chromatic aberration. </span>
<span>It is worth to mention that the outer segment of rods in nocturnal reptiles approaches a length of approximately four folds the length of the inner segments of the same photoreceptors cells. This character is of a particular interest, since the outer segment is the site of photopigments and the increase in its length magnifies its ability of light and consequently accommodate with the night vision. </span>
https://ejhm.journals.ekb.eg/article_18166.html
https://ejhm.journals.ekb.eg/article_18166_05a73d1b0bfc6792b878ce1b3ea1ed2b.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Effect of Systemic Lupus Erythematosus on some Biochemical Parameters in Female Patients
187
196
EN
Eman G.E
Helal
Zoology Department, Faculty of Science, Al- Azhar University (Girls)
Medhat
El- Shafaey
Clinical Immunology and Allergy Department, Faculty of Medicine, Ain- Shams University.
Hala
Rahoma
Clinical Immunology and Allergy Department, Faculty of Medicine, Ain- Shams University.
Mervat
Abdel- Rahman
Clinical Pathology Lab., Student Hospital, Cairo University.
10.21608/ejhm.2004.18167
<span>It has been noticed that there is an increase in the number of women suffering from SLE. Most studies confirmed that serum DHEA (dehydroepiandrosterone) and DHEA sulphate are lower among patients with SLE than among controls even- during phases of inactive disease so we performed this study to detect the level of DHEA-S in the female patients with SLE. The overall results confirm that DHEA treatment was well- tolerated, significantly reduced the number of SLE </span><span>flares, and improved patient’s global assessment of disease activity. Some serum parameters like </span><span>liver and kidney functions were detected. Biochemical analysis showed that there is a significant increase of total lipids, cholestrol, triglycerides. But insignificant change in serum glucose, urea nitrogen and uric acid levels were recorded. From the present study three things were concluded. Firstly, there is a strong relationship between level of DHEA and the progression of SLE. Secondly, liver activity and kidney functions were not affected by SLE disease.Thirdly, DHEA treatment has a beneficial effect on female patients. So this study recommended to follow up DHEAS in female patients and use it in a proper dosage. In addition, further study must be done. </span>
<span> </span>
SLE,DHEA,Liver functions,kidney functions
https://ejhm.journals.ekb.eg/article_18167.html
https://ejhm.journals.ekb.eg/article_18167_0b58fbe87cc31d02acfd87ee6662f452.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Effect Of L-Cysteine On Blood Picture And Some Serum Parameters In Rats Exposed To 2 Gauss Electro-Magnetic Field
197
206
EN
Mervat
Abdel-Rahman
Clinical pathology laboratory, Student hospital, Cairo University, Giza.
10.21608/ejhm.2004.18168
<span>Objective: </span><span>investigation of the bio-effects of exposure to 2 gauss electromagnetic field (EMF) on blood elements, blood glucose, hepatocellular enzymes and bilirubin of mice and their possible modification by L-cysteine. Methods: the following groups were studied; (1) normal rats treated with saline; (2) normal rats treated with L-cysteine (18 mg/kg); (3) rats exposed to EMF for 21 days and treated with the vehicle (saline) during the exposure period; (4) rats exposed to EMF for 21 days and treated with L-cysteine (18 mg/kg orally, 3 times per week) during the exposure period; (5) rats exposed to EMF for 21 days and treated with the vehicle (saline) during the exposure period and for 45 days after exposure; (6) rats exposed to EMF for 21 days and treated with L-cysteine (18 mg/kg orally, 3 times per week) during the exposure period and for 45 days after exposure; Results: in rats exposed to low frequency EMF for 21 days (group2), marked increase in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in serum was observed. Plasma bilirubin level was raised. Meanwhile, significant decrease in plasma glucose levels occured after exposure to EMF. No significant changes were observed in haemoglobin level, red blood cells or total leucocytic count noted in rats exposed to EMF. The elevations in serum bilirubin, AST and ALT levels were reduced to near normal values in rats given L-cysteine druing the 21 days of exposure (group3). On the other hand, in rats examined 45 days after the end of the exposure period (group 3), no significant alterations were noted as regards bilirubin, AST, ALT and glucose levels in serum. Conclusions: these results suggest that (1) exposure to low frequency EMF of 50 Hz is associated with some degree of liver injury reflected in increased leakage of hepatoceular enzymes into plasma as well as an increase in serum bilirubin; (2) these alterations can be ameliorated by the administration of L-cysteine, as well as; (3) by limiting exposure to EMF. </span>
<span> </span>
Electro-magnetic field,liver enzymes,rat,blood picture
https://ejhm.journals.ekb.eg/article_18168.html
https://ejhm.journals.ekb.eg/article_18168_54a38d5c9106919067a1b66d8cd382ef.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Bone Mineral Density In Different Stages Of Non Cholestatic Liver Cirrhoses
207
216
EN
Azza
Emam
Department of Internal Medicine Ain Shams University.
Omer
Hossian.
Department of Radio diagnosis, Ain Shams University.
Sherif .
Abou Gamrah
Department of Radio diagnosis, Ain Shams University.
10.21608/ejhm.2004.18169
<span>Hepatic osteodystrophy refers to metabolic Bone abnormalities observed in chronic liver disease. It is an important complication of chronic liver disease which includes osteoporosis and the much rare osteomalacia. It is varying from 13% to 70%, depending on the population studied and the diagnostic criteria used to define bone disease. </span>
<span>The advances in bone densitometry and the development of newer techniques, such as dual energy x-ray absorptiometry (DEXA), make it possible to rapidly and precisely quantify the amount of bone in the relevant fracture sites. DEXA is noninvasive, rapid, accurate, and safe. So it is the gold standard with which all other technologies are compared. </span>
<span>So in this study, BMD was measured using DEXA technique at 2 sites; antro-posterior lumbar spines and femoral neck in 30 cirrhotic patients and 10 healthy volunteers as a control group. </span>
<span>In addition routine laboratory investigations; CBC, ESR, Liver function tests, renal function tests, serum Na, K, Ca and Phosphorus, urinary 24 h Ca and viral markers; HBVs Ag and HCV Ab was done and abdominal U/S. </span>
<span>We concluded that liver cirrhosis is a direct and independent risk factor for bone loss which is mainly in the form of osteoporosis rather than osteomalacia and the degree of bone loss is related to severity of the liver disease as it worsens as the liver function does. The trabecular bone is more clearly affected than cortical bone. </span>
<span>So BMD should be measured in cirrhotic patients and management should be started in osteopenic and osteoporotic patients and follow up should be done. </span>
https://ejhm.journals.ekb.eg/article_18169.html
https://ejhm.journals.ekb.eg/article_18169_424872b7300a1b4c88d8b26040584fb6.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
17
1
2004
10
01
Asymptomatic Urinary Tract infection (UTI) among diabetic females
217
231
EN
Mona
Hosny
Internal Medicine Department, Ain Shams University
Yasser
Soliman
Internal Medicine Department, Ain Shams University
Ahmed
Abdel-Kader
Internal Medicine Department, Ain Shams University
Ahmed
Mohamed
Medical commission, Ain Shams University
10.21608/ejhm.2004.18170
<span>Our study was conducted on 1000 diabetic females of variable ages without symptoms of UTI. There were both type 1 and type 2 diabetic patients. </span>
<span>There were both married and unmarried females in both types of DM. </span>
<span>In addition to 100 normal females, which are age matched with patients group. They constituted control group. </span>
<span>Prevalence of ASB is significantly higher (P<0.01), by 4-5 folds in diabetic females than in normal ones. Several risk factors have been identified as glucosuria, proteinuria and duration of DM, whereas age, duration of marriage and seual activity are not proven to increase prevalence of ASB in diabetic females in our study. Repeated pregnancy times may be a risk factor for ASB in type 2 diabetic females (P<0.01). Staph. aureus was present in 54% of bacteriuric patients (with positive cultures) with either types of DM and E.coli was present in 30.8% of bacteriuric patients with either types of DM. Staph aureus is present in 45.9% of patients with type 1DM, while in type 2 DM, it was present in 59.1% of patients. E.Coli was isolated in 41.2% of patients with type 1 DM and it was present in 24.2% of patients with type 2 DM. </span>
https://ejhm.journals.ekb.eg/article_18170.html
https://ejhm.journals.ekb.eg/article_18170_7aa7e5be8079d53572d10c84a37841ca.pdf