ORIGINAL_ARTICLE
Mitochondria and Nuclear changes in postimplanted mice embryos After treatment with Cisplatim antitumour drugs
To evaluate a possible relationship of maternal exposure to capsulation during postimp- lantation embryotoxicity, SWR pregnant female mice were injected intraperitoneally on day 4 of gestation with 7mg/kg cisplatin or with saline (0.4 ml) for the control group. Embryos were collected after 24h 48h, 72h, after injection. ultrastructural sections were used to investigate the mitochondria and the nuclear changes Both the mitochondria and the nuclei were damaged specially 48h and 72 h after injection they may be related to cytotoxicity of the drug.
https://ejhm.journals.ekb.eg/article_18152_4111122c27138ad2d5da531c48a39aea.pdf
2004-10-01
1
11
10.21608/ejhm.2004.18152
platinum antitumour drugs
mitochondria
Nucleus
Mice embryos
Hassan M.
El Ashmaoui
1
National Research center, Cell Biology Dept., Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Reproductive and Developmental Effects of Moxifloxacin on Female Mice and Embryos
Moxifloxacin (Avelox®) is a fluoroquinolone antibiotic with a broad spectrum of activity and bactericidal action. Moxifloxacin has in vitro activity against a wide range of Gram- positive and Gram-negative organisms. The safe use of moxifloxacin in human pregnancy has not been established. In order to evaluate the genotoxic and embryo toxic effects of (Avelox)® during pregnancy, Avelox was administrated orally to female mice with doses (8.7, 17 and 26 mg/kg/day) from 1 to 17 days of pregnancy. Caesarean sections were completed on gestation day 18 and complete fetal examinations and cytogenetic analysis were conducted. Decreases in the fetal body weights and increases in the external visceral and skeletal anomalies were found in all doses of (8.7, 17 and 26 mg) Avelox compared to the controls. Cytogenetic analysis in mothers and embryos revealed that all the tests doses produced chromosomal aberrations and micronuclei (MN) formations in a dose dependent manner compared to the controls. These results indicate that Avelox has a maternal and embryotoxic effects on the female mice and their embryos when administered with a recommended and above the recommended dose during pregnancy.
https://ejhm.journals.ekb.eg/article_18153_610e991f85b671794018a4b68c881c39.pdf
2004-10-01
12
19
10.21608/ejhm.2004.18153
Moxifloxacin
chromosomal aberrations – micronuclei – mice – embryos
Hanaa M.
Roshdy
1
Cell Biology Department, National Research Center Dokki, Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
Ultrastructural study of renal tubular damage induced by captopril in adult and fetal mice
The present study has been designed to evaluate the possible nephrotoxicity of the angiotensin converting enzyme inhibitor, captopril on renal tubules of adult and maternally treated fetuses of CD-1 mice. The study included the effect of captopril administration for one month up to three months in adults, while in fetuses, they were exposed to the drug through their mothers in two periods. The first was from 6th-12th days of pregnancy, while the second was from 6th -18th day of pregnancy. The dose used in the present study represents the dose equivalent to the therapeutic daily dose taken by human. All the recorded tissue damage was found to be time dependent. The first remarkable feature noticed in all the treated adult animals was the presence of hyaline casts that obstructed most of the renal tubules. The second remarkable feature was the increase of the intertubular space associated with irregularity of the tubules due to the degeneration and vacuolation of the basal regions of the cells. Renal tubule cells showed large blebs, accumulaton of lipids, degeneration and necrosis. In maternally treated fetuses, the proximal convoluted tubule cells displayed moderate vacuolation and marked increase of lysosomes while some of the distal convoluted tubules revealed atrophy and their cells showed loss of mitochondria. In addition, the collecting tubules showed loss of microprojections. Worthy to mention that there was apparent increase of mesenchymal cells as well as fibroblasts in the fetuses maternally treated with captopril. The significance of these changes was discussed and it should be emphasized that captopril must be taken with caution for pregnant women and those who suffer from renal troubles. Moreover, kidney function should be monitored during therapy .
https://ejhm.journals.ekb.eg/article_18154_4a9063199fd67efc4d12b21cce888f3d.pdf
2004-10-01
20
43
10.21608/ejhm.2004.18154
Hamdy H.
Swelim
1
Department of Zoology, Faculty of Science, Ain Shams University
AUTHOR
Aleya A.
Sakr
2
Department of Zoology, Faculty of Science, Ain Shams University
AUTHOR
ORIGINAL_ARTICLE
Chemical, Nutritional and Microbiological Evaluation of Some Egyptian Soft Cheeses
Milk and dairy products is considered the most complete foodstuff that provide human either infants or adults with most of their vital needs. Milk and cheese have high nutritive value due to its high content of protein, fat, minerals especially calcium (Ca2+) & phosphorous, and vitamins. Two hundred samples produced and sold in Egypt during 2001-2003 were collected from allover the country. The cheese samples were subjected to microbiological and chemical analysis. Samples were microbiologically tested for total aerobic bacterial count (TABC), Colifrm, Escherichia coli (E. coli), Staphylococcus aureus, mould and yeast, salmonella and shigella, and listeria species. Protein, fat, carbohydrates, moisture, ash, lactose, Calcium (Ca), phosphorous (P) and Ca/P were evaluated. The analysis showed that total aerobic bacterial count did not exceed 1.4X105±1.7X105 cells/gm, which is close to what allowed by the Standard Egyptian Guidelines (2001) and 47.5 % of the tested cheese are free from coliform bacteria and Escherichia coli. Ninety-eight and half percent, 97 %, 97 % and 91.5 % of the tested cheese (kareish, feta, thalaga, double cream respectively), either made in plant or home or farmers’ cheese sample have zero Staphylococcus aureus count or mould and yeast; or salmonella and shigella, or listeria species respectively, i. e. free from them. Double cream cheese has the lowest protein content (7.79±0.78 gm%) while kareish cheese has the highest protein content (19.99±1.32 gm%), but for fat content the opposite is true, double cream cheese have the highest fat content (24.56±1.78 gm%) while kareish cheese have the lowest fat content (3.87±0.97 gm %). Feta cheese has high ash content while kareish cheese has the highest moisture content with the lowest ash content (68.97±1.86 & 1.81±0.47 gm% respectively). Lactose content varies widely from 1.50±0.26 (double cream cheese) to 3.25±0.50 (feta cheese). Kareish cheese has higher content of calcium and phosphorous (641.1±49.21 mg%, 431.18±37.21 mg% respectively) than the remaining types of cheese. Calcium & phosphorous content of kareish cheese is almost the double content of the double cream cheese. Feta cheese has higher Ca/P (1.65±0.19) while thalaga and double cream has lower Ca/P (1.34±0.13 & 1.37±0.20). Each 100 gm of soft cheese can provide children (1-8 y) & adult (9-≥50 y) from 39.78% & 24.48 % to128.22 % & 64.11% of their Ca Dietary Reference Intake and this from double cream cheese and kareish cheese respectively.
https://ejhm.journals.ekb.eg/article_18155_8a11f67fdc86bc0ffda062aa86e93645.pdf
2004-10-01
44
57
10.21608/ejhm.2004.18155
Ghada, Z.
A. A.
1
Lecture of Biochemistry The National Nutrition Institute
AUTHOR
Alia, M.
H
2
Lecture of Food Hygiene The National Nutrition Institute.
AUTHOR
Soha, .
Al-S
3
Lecture of Nutrition Suez Canal University.
AUTHOR
Magdy,
N. A.
4
Associate Prof of Ecological Sciences The National Nutrition Institute.
AUTHOR
Mohammed,
F. S.,
5
Prof of Microbiology The National Nutrition Institute.
AUTHOR
ORIGINAL_ARTICLE
Possible Association Between the Chemokine Receptor Gene CCR5- Delta32 Mutation and Hepatitis C Virus Pathogenesis
Background: CCR5-Delta32, a 32-base pair deletion of the CC chemokine receptor (CCR)5 gene, is associated with slowed human immunodeficiency virus disease progression in heterozygotes and protection against infection in homozygotes between carriers and non-carriers of each genetic variant. The present study investigated the frequency and clinical consequence of the CCR%-Delta32 mutation in Egyptian HCV infected patients. Genomic DNA samples from 150 patients with chronic HCV infection were screened by PCR for the presence of the CCR5-Delta32 polymorphism. One hundred blood donors were used as control population. Results: The frequency of CCR5-Delta32 heterozygosity was 0.67% in chronic hepatitis C virus and 0% in controls. The CCR5-Delta32 allele was not associated with any of the clinical parameters of hepatitis C virus infection. Conclusion: In this study, the frequency of CCR5-Delta32 homozygosity in patients with hepatitis C was similar to controls.
https://ejhm.journals.ekb.eg/article_18157_d1d3c7c86c049b893e6382b0703d6cba.pdf
2004-10-01
58
62
10.21608/ejhm.2004.18157
Kouka Saad Eldin
Abdel-Wahab
1
Department of Medical Microbiology, Faculty of Medicine for Girls,
AUTHOR
Mohamed
Foda
2
Department of Medical Virology School of Medicine, Kumamoto University, Japan;
AUTHOR
Magda Abdel- Moneim
Gamil
3
Department of Medical Microbiology, Faculty of Medicine for Girls
AUTHOR
Azza-Hassan
El-salakawy
4
Department of Medical Microbiology, Faculty of Medicine for Girls
AUTHOR
Gamal
El-Attar
5
Department of Internal Medicine, Theodore Bilharz Institute, Imbaba, Egypt
AUTHOR
Shinji
Harada
6
Department of Medical Virology School of Medicine, Kumamoto University, Japan
AUTHOR
Yosuke
Maeda
7
Department of Medical Virology School of Medicine, Kumamoto University, Japan
AUTHOR
ORIGINAL_ARTICLE
Cytogenetic and Developmental Effects of Antidepression Drug (Cipralex) on Female Mice and Embryos
Escitalopram (cipralex®) a new highly selective serotonin reuptake inhibitor, it is effective in the treatment of patients with major depression. To evaluate the cytogenetics and developmental effects of cipralex throughout major organgenesis, mice were administrated orally with a doses of 0.06, 0.12 and 0.24 mg/kg/day cipralex on gestation days 1-18 and examined on the 19th day of gestation for evidence of maternal and fetal toxicity. Cipralex at different doses tested produce significant toxic effects in reproductive parameters. Significant embryo fetotoxic effects were observed at tested dose levels as evidenced by total number of implantations, post. Implantation loss and embryo malformations. There were increases in the frequencies of micronuclei and chromosomal aberrations in both maternal and embryonic cells treated with cipalex, these increases were dose dependent. These results indicate that cipralex is considered to be cytogenetic and embryo toxic drug when administered during pregnancy.
https://ejhm.journals.ekb.eg/article_18158_5a38a496ea7c15b9cc370c748051fd6a.pdf
2004-10-01
63
69
10.21608/ejhm.2004.18158
Escitalopram
chromosomal aberrations – micronuclei – embryo – mice
Hanaa M.
Roshdy
1
Cell Biology Department, National Research Center Dokki, Cairo, Egypt
AUTHOR
Thanaa M.T
Shoman
2
Cell Biology Department, National Research Center Dokki, Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
L -Carnitine and Melatonin Reverse CCl4 Induced Liver Fibrosis In Rats (Histological and Histochemical Studies)
Carbontetrachloride (CCl4) is closely related chemically to chloroform and likewise in hepatic poisons. This study was designed to evaluate the effects of carbon tetrachloride on liver of male rats and the reversing effects of L-carnitine and melatonin on established liver fibrosis. A total of 72 adult male albino rats were used in this study. The animals were divided into six groups. Group (1) animals of the first group were kept as control andtreated with paraffin oil twice weekly for eight weeks. Group (2) rats of the second group were injected with CCl4 intraperitoneally at 0.15 ml per rats (diluted 1:1 in liquid paraffin) twice weekly for eight weeks to produced liver fibrosis. Group (3) following establishment with CCl4 which induced liver fibrosis, the rats were treated with L-carnitine at a dose level of 50 mg/kg for four weeks. Group (4) rats with liver fibrosis were injected intraperitoneally with melatonin at dose level of 10 mg/kg for four weeks. The fifth and sixth groups were given L-carnitine and/or melatonin at dose levels of 50 mg/kg and 10 mg/kg respectively for four weeks.
Histological changes in the liver of rats treated with CCl4 including liver fibrosis, architecture distortion and appearance of many pseudolobule. The fibrous tissues run in septa between the nodules. The liver damage varied from one area to another and varied from moderate fibrosis to cirrhosis.
Quantitative measurement of the severity of liver fibrosis (area damage) was achieved by using computerized image analysis (Leica image) showed that highly significant increase in area of fibrosis was recorded in the case of rats treated with CCl4 only.
Quantitative DNA image analysis showed that 3% of aneuploid cells could be noticed in liver of rats treated with CCl4 only. Histochemical results of rats treated with CCl4 showed highly significant increase in grey level of mucopolysaccharides and protein levels. No histological and histochemical changes could be noticed in the liver of rats treated with either L- carnitine or melatonin only. Both L–carnitine and melatonin were found to reverse CCl4 induced liver damage.
https://ejhm.journals.ekb.eg/article_18159_da1d3e0480b76ce2607305efb6ac6156.pdf
2004-10-01
70
92
10.21608/ejhm.2004.18159
L-carnitine
Melatonin
carbontetrachloride
Liver fibrosis
Rats
Histological
histochemical
Fatma, A.
Morsy
1
AUTHOR
Abdel Razik, H.
Farrag
2
AUTHOR
Sonya, L.
El-Sharkawy
3
AUTHOR
ORIGINAL_ARTICLE
Biological effects of ivermectin on the fowl tick Argas (Persicargas) persicus (Oken) (Ixodoidea: Argasidae)
The present study is carried out to assess the effect induced by different single subcutaneous injections of ivermectin (IVM) (100, 200, 400, 800 or 1600 μg/kg pigeon weight) injected 2 or 7 days before tick feeding on some biological parameters such as mobility and viability, sexual activity, ingested blood, amount of coxal fluid, blood digestion and fertility in the tick Argas (P.) persicus to define the effective dose. This effective dose was used in similar assessment conducted 2 or 3 weeks post injection in order to confirm the degradation of ivermectin concentration in the host blood and to determine the number of required doses for complete control . From this study we conclude: 1) IVM induces complete immobilization of both males and females when they are fed on hosts injected by doses over 100 μg/kg. 2) The use of two doses of 400 μg/kg with a week interval completely controls the tick population. 3) Sexual response was completely negative at doses over 200 μg/kg. 4) The amount of coxal fluid emitted by both sexes decreased markedly when fed after host injection with all doses, whereas the amount of ingested blood remained generally not highly affected. 5) The number of ovipositing females, number of eggs deposited and their hatching percent decreased markedly with the increase of dose used. Blood digestion was not noticed in males at doses >200 ug/kg and in females at doses >100ug/kg .
https://ejhm.journals.ekb.eg/article_18160_d40ccc12d582fffbbff9d187dda1abd8.pdf
2004-10-01
93
105
10.21608/ejhm.2004.18160
Argas
Efficacy
Ivermectin
Marzouk,
A.S
1
Department of Zoology, Faculty of Science, Ain Shams University
AUTHOR
Swelim,
H.H
2
Department of Zoology, Faculty of Science, Ain Shams University
AUTHOR
Montasser,
A.A
3
Department of Zoology, Faculty of Science, Ain Shams University
AUTHOR
Gadallah,
A.I
4
Department of Medical Entomology, Faculty of Agriculture, Al-Azhar University
AUTHOR
ORIGINAL_ARTICLE
Acute Hepatitis E. virus infection in Egypt
Hepatitis E virus (HEV) is largely responsible for water borne epidemics in many developing countries. The principle mode of HEV transmission is the fecal oral route in epidemic and sporadic forms with a high case fatality ratio in pregnant women. Serum samples from 50 healthy subjects and from 435 acute viral hepatitis patients, 4-75 years old, were screened for markers of acute viral hepatitis. These included (HBsAg, anti-HBc (IgM), anti- HDV (IgM), HAV (IgM), anti-HCV (IgG), and anti-HEV (IgG), and (IgM) tests by enzyme- linked immunoassays (EIA).
Furthermore isolation of HEV from peripheral blood lymphocytes and from stools belonging to anti-HEV IgG-positive patients was attempted by inoculation of HepG2 and Vero cell line cultures. The inoculated cell cultures were examined after immunoperoxidase staining for the detection of HEV antigen. Plasma, lymphocytes and stool samples from anti-HEV IgM positive patients were examined for HEV RNA by PCR. Anti-HEV IgG was found in 144/435 (33%) of these acute hepatitis patients. Anti-HEV (IgM) was detected in 8/52 (15.4%) out of 52 chosen from the 144 sera that were anti-HEV IgG positive cases.
HEV was isolated in HepG2 from 32.6% of lymphocyte and from 34.9% of stools from patients positive for anti-HEV (IgG). While it was isolated from 71.4% of lymphocytes and from 100% of stools from patients positive for anti-HEV (IgM). In Vero cell cultures there was no HEV isolation from stools but HEV was isolated from 50% of lymphocytes. HEV RNA was detected by PCR in 85.7% of stools, 62.5% of plasma, and in 37.5% of lymphocyte samples belonging to anti-HEV IgM positive cases. Analysis of these diagnostic tests indicated that virus isolation from peripheral blood lymphocytes and stools by inoculation of HepG2 cell cultures is more sensitive than virus-RNA detection by PCR
https://ejhm.journals.ekb.eg/article_18161_8088a36a59ffaf74296fbc456fd5e896.pdf
2004-10-01
106
114
10.21608/ejhm.2004.18161
Osman
F
1
Abbassia Fever Hospital, Ministry of Public Health
AUTHOR
Abu-Shady
EA
2
Microbiology Department, Faculty of Medicine – Al-Azhar University (Girls)
AUTHOR
Abd El-Wahab
KSE
3
Microbiology Department, Faculty of Medicine – Al-Azhar University (Girls)
AUTHOR
El-Rashidy
ZE
4
Microbiology Department, Faculty of Medicine – Al-Azhar University (Girls)
AUTHOR
ORIGINAL_ARTICLE
Ultrastructural Studies of The Pigment Epithelium of Retinae of Some Reptiles
The present work was designed to reveal the fine structure of the pigment epithelium of the retina in four different reptiles: the homed viper Cerastes cerastes (diurnal and nocturnal), the European Chameleo chameleon (diurnal),the gold skink Eumeces schenrdii (diurnal) and the Egyptian gecko Traentola annularis (nocturnal) . The pigment epithelium of each type reptiles possessed certain unique features in their morphology and ultrastructure to accommodate with their day and night activity. The most striking feature was the presence of myoid bodies in the pigment epithelium of the diurnal reptiles. These bodies trigger the photomechanical movement of the myoid region in cones of their retinae.
https://ejhm.journals.ekb.eg/article_18162_401ea5c38abf17154b249259d248af63.pdf
2004-10-01
115
129
10.21608/ejhm.2004.18162
Fairoze
Khattab
1
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
AUTHOR
Fahmy I.
Khattab
2
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
AUTHOR
Nagui
Fares
3
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
AUTHOR
Aman
Zaki
4
Department of Zoology, Faculty of Science ,Ain Shams University, Abbassia, Cairo, Egypt.
AUTHOR
ORIGINAL_ARTICLE
Immunohistochemical Study Of Bcl-2 Protein And Estrogen Receptor-Alpha Expression In Benign Prostatic Hyperplasia And Prostatic Carcinoma
The human prostate, a male sexual accessory tissue involved in seminal fluid production, has a remarkably high incidence of hyperplastic and neoplastic disease. The present study was carried out on one hundred and twenty (120) specimens divided into two groups; group 1: Included forty cases of benign prostatic hyperplasia (BPH) and group 2: Included sixty cases of prostatic adenocarcinoma (PC) (22 were low grade; GS: 2-6 and 38 were high grade; GS: 7- 10),in addition to twenty cases of histologically normal prostates taken as controls. Immunohistochemical technique was applied to detect Bcl-2 as well as ER α positivity in all specimens. Group 1 showed the following profile: ER α (+) in all cases (100%), Bcl-2 (-) in 95%, ERα (+) / Bcl-2 (+) in 95%, ERα (-) / Bcl-2 (+) in 0%, ERα (+) / Bcl-2 (-) in 5% and ERα (-) / Bcl-2 (-) in 0% of cases while group 2 showed the following profile: ERα (+) in 30%, Bcl-2 (+) in 21.7%, ERα (+) / Bcl-2 (+) in 15%, ERα (-) / Bcl-2 (+) in 6.7%, ERα (+) / Bcl-2 (-) in 15% and ERα (-) / Bcl-2 (-) in 70% of cases. The mean epithelial ER α -immunolabeling was, however, significantly increased in group 2 than in group 1 (P < 0.05) which, in turn, being higher than the normal cases (P<0.05 ) . Among group 2 , the mean ER α was significantly more in high grade than in low grade tumors (P < 0.05), however, the mean ER α immunolabeling revealed no significant correlation with T-stage (P = 0.219) or with the clinical stage (P = 0.391). In contrast, the Bcl-2 immunostaining was statistically higher in group 1 than in group 2 (P < 0.05) and showed a significant correlation with T stage (P < 0.05) although the study displayed no significant correlation between Bcl-2 immunopositivity and either Gleason score (P = 0.125) or the histologic grade (P = 0.146). In addition, combined ER α (+)/ Bcl 2 (+) immunoreactivity demonstrated the aggressive subgroup of PC cases more accurately than either ER α (+) or Bcl-2 (+) alone. Finally, multivariate analysis showed that the Bcl-2, proved to be an independent prognostic indicator (P < 0.05). Thus, the immunohistochemical expression of ER α and Bcl-2 protein in prostatic tissue may aid in better understanding the biology and genesis of both prostatic hyperplasia and carcinoma .
https://ejhm.journals.ekb.eg/article_18163_c014df1f9aaf9e2bf1511f1f83071a38.pdf
2004-10-01
130
142
10.21608/ejhm.2004.18163
Bcl-2
Estrogen receptor-alpha
Immunohistochemistry
Benign Prostatic Hyperplasia
prostatic carcinoma
Ahmed H. Abel-Rahman- Ghada A.
Abdel-Aziz
1
Departments of Pathology, Dermatology & Veneriology Al-Azhar University (Assiut & Girls)
AUTHOR
Ali Emad
S
2
Urology Al-Azhar University (Assiut & Girls)
AUTHOR
Basset A.
Badawy
3
South Valley University
AUTHOR
Alaa Ar.
Abdel-Hafez
4
South Valley University
AUTHOR
ORIGINAL_ARTICLE
Mutagenic studies on the effect of Aldicarb “Temik” and vitamin C as antioxidant agent on the white rat: (Chromosomal aberrations and Micronucleus tests)
Widespread contamination of the environment due to increased and frequently indiscriminate usage of insecticides during the last two decades has aroused much concern over the possibility of their radiominetic effect. Evidence accumulating over the years emphasized the indisputable link between certain insecticides, chromosomal damage and possibility of gene mutation. There is a wide variety of insecticides, among which the carbamates. Their chemical relationship to ethyl carbamate makes them worthy of study for their possible deleterious effect on biological system. The main object of the present study is to evaluate the mutagenic effect of a carbamate insecticide” Aldicarb” alone and in combination of vitamin C as an antioxidant agent to decrease their mutagenicity. Male albino rats were tested orally for 48 hours , two doses of aldicarb were used in absence and in the presence of viamin C (1/4 and 1/10) LD50. The obtained data showed highly significant increase in the micronucleus (PCEM) and in chromosomal aberrations in rat bone marrow cells at the two doses of aldicarb compared to control group. (P< 0.0001). The frequency of chromosomal aberrations and micronucleus decreased in rats treated with aldicarb and vitamin C than in aldicarb treated group. From these results we concluded that cytogenetic effect of aldicarb might be decreased by the usage of vitamin as an antioxidant agent.
https://ejhm.journals.ekb.eg/article_18164_66e76188c63bf2c1e6c3a1bb4e31540e.pdf
2004-10-01
143
154
10.21608/ejhm.2004.18164
Carbamate
Aldicarb
Chromosomal aberration
micronucleus
Antioxidant agents
Fatma M.
Hamam
1
Department Of Mammalian Toxicology, Central Agricultural Pesticides Laboratory
AUTHOR
Ihab H.
Foda
2
Agricultural Research Center, Ministry Of Agriculture
AUTHOR
ORIGINAL_ARTICLE
Evaluation Of Hydatid Disease (Echinoccosis) In Algmeil Hospital (2002 – 2003)
Hydatidosis is a zoonotic parasitic disease caused by the dog tapeworm Echinococcus and its larval stage, the hydatid cyst. Humans can accidentally become intermediate hosts by ingesting the eggs of the tapeworm. While most cysts deve lop in the liver and lungs. animals. At present, four species of the genus Echinococcus are recognized and regarded as taxonomically valid: E. granulosus (cystic hydatidosis), E. multilocularis (multivesicular hydatidosis), E. vogeli (polycystic hydatidosis) and E. oligarthrus (Soulsby, 1982). A total number of 23 patients were included in this study. 13 patients were females while the rest 10 were male patients. All cases were properly diagnosed as Hydated disease and then treated in the surgery Department of Algmeil Hospital (Libia) in the last 2 years (2002 and 2003). Proper investigations as well as treatment were carried out. The obtained results were statistically analyzed. Four types of presentation of the disease were observed in this study and presented, Asymptomatic 78.26%, Obstructive jaundice 8.69%, Accidental rupture 8.69% and Pressure symptom 4.34%. In spite of the progress in these areas, echinococcus/ hydatidosis remains a serious public health problem in a number of countries. It is very important to support and implement new control programmes so as to prevent further spread of the disease. Research in possible vaccines is essential in order to supplement the existing methods of breaking the Echinococcus life cycle.
https://ejhm.journals.ekb.eg/article_18165_c2db642578d3c7a65a28be9fb6ae7b22.pdf
2004-10-01
155
166
10.21608/ejhm.2004.18165
Abdel-Hakim
Rezeeg
1
Assistant Professor Of Surgery AlgmeilHospital- Libia
AUTHOR
ORIGINAL_ARTICLE
Retinal Photoreceptor Fine Structure in some reptiles
The structure of the photoreceptors of four different reptiles: the homed viper Cerastes cerastes (diurnal and nocturnal), the European Chameleo chameleon (diurnal), the gold skink Eumeces schneidrii (diurnal) and the Egyptian gecko, Tarentola annularis (nocturnal) has been investigated by light and electron microscopy.
The photoreceptors of diurnal reptiles were mainly of the cone type and those of nocturnal were mainly rods. The ellipsoid region of both double rods in the nocturnals and large single cones in the species having both nocturnals and diurnal activity, consist of several mitochondria arranged in a remarkable radially gradient architecture which accommodates with the specific function of this region as a focusing device helping to condense light onto the outer segments. Moreover the principle cone of double cone and single cone of diurnal reptiles possessed a large oil droplet in the region between the inner segment and outer segment. This droplet is thought to play a role in filtering light and so doing enhanced contrast reduce glare and lessen chromatic aberration.
It is worth to mention that the outer segment of rods in nocturnal reptiles approaches a length of approximately four folds the length of the inner segments of the same photoreceptors cells. This character is of a particular interest, since the outer segment is the site of photopigments and the increase in its length magnifies its ability of light and consequently accommodate with the night vision.
https://ejhm.journals.ekb.eg/article_18166_05a73d1b0bfc6792b878ce1b3ea1ed2b.pdf
2004-10-01
167
186
10.21608/ejhm.2004.18166
Fairoze
Khattab
1
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
AUTHOR
Fahmy I.
Khattab
2
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
AUTHOR
Nagui
Fares
3
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
AUTHOR
Aman
Zaki
4
Department of Zoology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt.)
AUTHOR
ORIGINAL_ARTICLE
Effect of Systemic Lupus Erythematosus on some Biochemical Parameters in Female Patients
It has been noticed that there is an increase in the number of women suffering from SLE. Most studies confirmed that serum DHEA (dehydroepiandrosterone) and DHEA sulphate are lower among patients with SLE than among controls even- during phases of inactive disease so we performed this study to detect the level of DHEA-S in the female patients with SLE. The overall results confirm that DHEA treatment was well- tolerated, significantly reduced the number of SLE flares, and improved patient’s global assessment of disease activity. Some serum parameters like liver and kidney functions were detected. Biochemical analysis showed that there is a significant increase of total lipids, cholestrol, triglycerides. But insignificant change in serum glucose, urea nitrogen and uric acid levels were recorded. From the present study three things were concluded. Firstly, there is a strong relationship between level of DHEA and the progression of SLE. Secondly, liver activity and kidney functions were not affected by SLE disease.Thirdly, DHEA treatment has a beneficial effect on female patients. So this study recommended to follow up DHEAS in female patients and use it in a proper dosage. In addition, further study must be done.
https://ejhm.journals.ekb.eg/article_18167_0b58fbe87cc31d02acfd87ee6662f452.pdf
2004-10-01
187
196
10.21608/ejhm.2004.18167
SLE
DHEA
Liver functions
kidney functions
Eman G.E
Helal
1
Zoology Department, Faculty of Science, Al- Azhar University (Girls)
AUTHOR
Medhat
El- Shafaey
2
Clinical Immunology and Allergy Department, Faculty of Medicine, Ain- Shams University.
AUTHOR
Hala
Rahoma
3
Clinical Immunology and Allergy Department, Faculty of Medicine, Ain- Shams University.
AUTHOR
Mervat
Abdel- Rahman
4
Clinical Pathology Lab., Student Hospital, Cairo University.
AUTHOR
ORIGINAL_ARTICLE
Effect Of L-Cysteine On Blood Picture And Some Serum Parameters In Rats Exposed To 2 Gauss Electro-Magnetic Field
Objective: investigation of the bio-effects of exposure to 2 gauss electromagnetic field (EMF) on blood elements, blood glucose, hepatocellular enzymes and bilirubin of mice and their possible modification by L-cysteine. Methods: the following groups were studied; (1) normal rats treated with saline; (2) normal rats treated with L-cysteine (18 mg/kg); (3) rats exposed to EMF for 21 days and treated with the vehicle (saline) during the exposure period; (4) rats exposed to EMF for 21 days and treated with L-cysteine (18 mg/kg orally, 3 times per week) during the exposure period; (5) rats exposed to EMF for 21 days and treated with the vehicle (saline) during the exposure period and for 45 days after exposure; (6) rats exposed to EMF for 21 days and treated with L-cysteine (18 mg/kg orally, 3 times per week) during the exposure period and for 45 days after exposure; Results: in rats exposed to low frequency EMF for 21 days (group2), marked increase in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in serum was observed. Plasma bilirubin level was raised. Meanwhile, significant decrease in plasma glucose levels occured after exposure to EMF. No significant changes were observed in haemoglobin level, red blood cells or total leucocytic count noted in rats exposed to EMF. The elevations in serum bilirubin, AST and ALT levels were reduced to near normal values in rats given L-cysteine druing the 21 days of exposure (group3). On the other hand, in rats examined 45 days after the end of the exposure period (group 3), no significant alterations were noted as regards bilirubin, AST, ALT and glucose levels in serum. Conclusions: these results suggest that (1) exposure to low frequency EMF of 50 Hz is associated with some degree of liver injury reflected in increased leakage of hepatoceular enzymes into plasma as well as an increase in serum bilirubin; (2) these alterations can be ameliorated by the administration of L-cysteine, as well as; (3) by limiting exposure to EMF.
https://ejhm.journals.ekb.eg/article_18168_54a38d5c9106919067a1b66d8cd382ef.pdf
2004-10-01
197
206
10.21608/ejhm.2004.18168
Electro-magnetic field
liver enzymes
rat
blood picture
Mervat
Abdel-Rahman
1
Clinical pathology laboratory, Student hospital, Cairo University, Giza.
AUTHOR
ORIGINAL_ARTICLE
Bone Mineral Density In Different Stages Of Non Cholestatic Liver Cirrhoses
Hepatic osteodystrophy refers to metabolic Bone abnormalities observed in chronic liver disease. It is an important complication of chronic liver disease which includes osteoporosis and the much rare osteomalacia. It is varying from 13% to 70%, depending on the population studied and the diagnostic criteria used to define bone disease.
The advances in bone densitometry and the development of newer techniques, such as dual energy x-ray absorptiometry (DEXA), make it possible to rapidly and precisely quantify the amount of bone in the relevant fracture sites. DEXA is noninvasive, rapid, accurate, and safe. So it is the gold standard with which all other technologies are compared.
So in this study, BMD was measured using DEXA technique at 2 sites; antro-posterior lumbar spines and femoral neck in 30 cirrhotic patients and 10 healthy volunteers as a control group.
In addition routine laboratory investigations; CBC, ESR, Liver function tests, renal function tests, serum Na, K, Ca and Phosphorus, urinary 24 h Ca and viral markers; HBVs Ag and HCV Ab was done and abdominal U/S.
We concluded that liver cirrhosis is a direct and independent risk factor for bone loss which is mainly in the form of osteoporosis rather than osteomalacia and the degree of bone loss is related to severity of the liver disease as it worsens as the liver function does. The trabecular bone is more clearly affected than cortical bone.
So BMD should be measured in cirrhotic patients and management should be started in osteopenic and osteoporotic patients and follow up should be done.
https://ejhm.journals.ekb.eg/article_18169_424872b7300a1b4c88d8b26040584fb6.pdf
2004-10-01
207
216
10.21608/ejhm.2004.18169
Azza
Emam
1
Department of Internal Medicine Ain Shams University.
AUTHOR
Omer
Hossian.
2
Department of Radio diagnosis, Ain Shams University.
AUTHOR
Sherif .
Abou Gamrah
3
Department of Radio diagnosis, Ain Shams University.
AUTHOR
ORIGINAL_ARTICLE
Asymptomatic Urinary Tract infection (UTI) among diabetic females
Our study was conducted on 1000 diabetic females of variable ages without symptoms of UTI. There were both type 1 and type 2 diabetic patients.
There were both married and unmarried females in both types of DM.
In addition to 100 normal females, which are age matched with patients group. They constituted control group.
Prevalence of ASB is significantly higher (P<0.01), by 4-5 folds in diabetic females than in normal ones. Several risk factors have been identified as glucosuria, proteinuria and duration of DM, whereas age, duration of marriage and seual activity are not proven to increase prevalence of ASB in diabetic females in our study. Repeated pregnancy times may be a risk factor for ASB in type 2 diabetic females (P<0.01). Staph. aureus was present in 54% of bacteriuric patients (with positive cultures) with either types of DM and E.coli was present in 30.8% of bacteriuric patients with either types of DM. Staph aureus is present in 45.9% of patients with type 1DM, while in type 2 DM, it was present in 59.1% of patients. E.Coli was isolated in 41.2% of patients with type 1 DM and it was present in 24.2% of patients with type 2 DM.
https://ejhm.journals.ekb.eg/article_18170_7aa7e5be8079d53572d10c84a37841ca.pdf
2004-10-01
217
231
10.21608/ejhm.2004.18170
Mona
Hosny
1
Internal Medicine Department, Ain Shams University
AUTHOR
Yasser
Soliman
2
Internal Medicine Department, Ain Shams University
AUTHOR
Ahmed
Abdel-Kader
3
Internal Medicine Department, Ain Shams University
AUTHOR
Ahmed
Mohamed
4
Medical commission, Ain Shams University
AUTHOR