ORIGINAL_ARTICLE
Depression among type 2 diabetic patients
Background & Objectives: Diabetes and depression are highly prevalent conditions and have significant impact on health outcomes. The combination of depression with type 2 diabetes is a public health problem. Therefore, we aimed to assess some socio-demographic characteristics of type 2 diabetes and to investigate the relationship between type 2 diabetes and depression among patients aged from 40 to 60 years old.
Methods: 125 patients diagnosed with type 2 diabetes attending diabetes clinics in the Al-Zahraa hospital were invited to participate in this cross-sectional study. Patients were interviewed using structured questionnaires to gather data on socio-demographics, clinical, self care compliance, medication usage, and diabetes complications. The MADRS was administered as a screening tool for depression level evaluation. Binary logistic regression model was used to examine association between predictor variables and risk of depression among diagnosed type 2 diabetes at 95% C.I. and P < 0.05.
Results: One hundred and twenty five participants completed the interview. More than half of participants were females (58.4%) and the mean age was 48 (sd = 5.9), 47.2% hypertensive, and 59.2% on insulin. More than two third (74.4%) of patients were depressed; (24.8% mild, 37.6% moderate and 12% severely depressed). Almost four out of five patients (88.8%) had diabetes complications, Depression was strongly associated with neuropathy, age, retinopathy, sex and cardiac complications. However, the likelihood of depression was not associated with nephropathy, hypertension and sexual dysfunction.
Conclusion: The current study demonstrates a strong correlation between depression and diabetes particularly complications. In particular, patients who are depressed tended to have poorer self-care, more severe physical symptoms and were less likely to adhere to prescribed care regimens. These findings raise the possibility that improving the mental health as part of a comprehensive management plan for diabetes may improve the overall long term outcomes of these patients.
https://ejhm.journals.ekb.eg/article_16406_3e7c84ee073b3f61cde1059231c7394f.pdf
2011-07-01
258
271
10.21608/ejhm.2011.16406
depression • diabetes mellitus. MADRS
Taghreed Mohamed
El-Shafie
1
Psychiatry Department Faculty of Medicine for Girls, Al-Azhar University
AUTHOR
Entesar Omar A.
El-Saghier
2
Endocrinology Department Faculty of Medicine for Girls, Al-Azhar University
AUTHOR
Iman Kamal
Ramadan
3
Community Department Faculty of Medicine for Girls, Al-Azhar University
AUTHOR
ORIGINAL_ARTICLE
The effect of the non-steroidal anti-inflammatory drug diclofenac sodium on the fetuses of albino mice
Introduction: The present study was carried out to evaluate the effect of the non-steroidal anti-inflammatory drug diclofenac sodium (DS) on the fetuses of albino mice from the morphological and skeletal points of view.
Material and methods: Sixty adult pregnant female mice were used in the present study. They were allocated into 6 groups (10 mice each). The first two groups served as control and were injected intraperitoneally (i.p.) with the solvent of the drug, and the 3rd and 5th groups were treated with 1.5 and 3mg/kg body weight of diclofenac sodium for 6 days ( gestation days 1-6 ), respectively ; the 4th and 6th groups were treated with 1.5and 3mg/kg body weight of the drug for 8 days ( gestation days 7-14), respectively.
Results: The morphological examination of the fetuses of treated groups showed conspicuous decrease in the average body weight and body length in all treated groups. The fetuses maternally treated with the drug showed noticeable external morphological malformations and their skeletons exhibited mild retardation in skeletal elements.
In conclusion: The non-steroidal anti-inflammatory drug diclofenac sodium had exerted marked morphological malformations and mild skeletal alterations in mice fetuses maternally treated during different periods of gestation.
Introduction: The present study was carried out to evaluate the effect of the non-steroidal anti-inflammatory drug diclofenac sodium (DS) on the fetuses of albino mice from the morphological and skeletal points of view.
Material and methods: Sixty adult pregnant female mice were used in the present study. They were allocated into 6 groups (10 mice each). The first two groups served as control and were injected intraperitoneally (i.p.) with the solvent of the drug, and the 3rd and 5th groups were treated with 1.5 and 3mg/kg body weight of diclofenac sodium for 6 days ( gestation days 1-6 ), respectively ; the 4th and 6th groups were treated with 1.5and 3mg/kg body weight of the drug for 8 days ( gestation days 7-14), respectively.
Results: The morphological examination of the fetuses of treated groups showed conspicuous decrease in the average body weight and body length in all treated groups. The fetuses maternally treated with the drug showed noticeable external morphological malformations and their skeletons exhibited mild retardation in skeletal elements.
In conclusion: The non-steroidal anti-inflammatory drug diclofenac sodium had exerted marked morphological malformations and mild skeletal alterations in mice fetuses maternally treated during different periods of gestation.
https://ejhm.journals.ekb.eg/article_16407_1a585bf87b52645b81d41a32c4c256f7.pdf
2011-07-01
272
283
10.21608/ejhm.2011.16407
Diclofenac sodium- Morphology – Skeleton- Fetuses of albino mice
Mohamed A.
Shahin
1
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
AUTHOR
Ramadan A.
Ramadan
2
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
AUTHOR
Samia M.
Sakr
3
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
AUTHOR
Sahar A.
Sabry
4
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
AUTHOR
ORIGINAL_ARTICLE
Protective effects of sulpiride treatment on kidney functions of female albino rats exposed to noise stress.
Background: Noise is the most stressful factor for experimental animals. So these studies aim to clarify its effect on some physiological and histological parameters.
Material and methods: 36 Female rats were divided into four groups (6/each):1-control, 2-treated with sulpiride drug,3- noise exposure (90db/3h per day for 30days), 4-noise + drug
Results: drug recorded no significant change in all the studied parameters. Noise stress recorded a significant increase in creatinine, total lipids, TG, Cholesterol, HDL-C, LDL-C and no significant changes in urea, uric acide.
It has been detected that sulpiride drug ameliorated most of these parameters.Concerning the histological and histochemical studies sulpiride treatment showed no detectable changes in the kidney tissue with exception of increased lymphocytes. Exposure to noise showed many dystrophic changes in the kidney tissue, but drug treatment improved all the previous changes and this indicates the protective effect of sulpiride against noise exposure.
Conclusion: It is useful to use sulpiride drug in people who exposed to noise stress.
https://ejhm.journals.ekb.eg/article_16408_be71e8fbecaf7e6e01cf326bde2eecc3.pdf
2011-07-01
284
294
10.21608/ejhm.2011.16408
Noise
Sulpiride drug
albino rats
physiological parameters
Histopathalogy and histochemistery
Eman G
.Helal
1
Zoology Department ,Faculty of Science,Al-Azhar University. ( Physiology)
AUTHOR
Fatma
Eid
2
Zoology Department ,Faculty of Science,Al-Azhar University. ( Histology)
AUTHOR
Neama M.
Taha.
3
Zoology Department ,Faculty of Science,Al-Azhar University
AUTHOR
ORIGINAL_ARTICLE
Effect of noise stress and /or sulpiride treatment on some physiological and histological parameters in female albino rats.
Background: Noise is the most stressful factor and human being. So these studies aim to clarify its effect on some physiological and histological parameters.
Material and methods: 24 Female rats were divided into four groups (6/each):1-control, 2-treated with sulpiride drug,3- noise exposure (90db/3h per day for 30days), 4-noise + drug
Results: drug recorded a significant increase only in the percentage of body weight gain but the other parameters showed no significant changes. Noise stress recorded a significant increase in glucose, ALT, GGT, TP, globulin.
A significant decrease in percentage of body weight gain and A/G ratio were also detected . It has been detected that Sulpiride drug ameliorated most of these parameters.
Concerning the histological and histochemical studies sulpiride treatment showed no detectable changes in the liver tissues with exception of increased lymphocytes. Exposure to noise showed many dystrophic change in tissue, but drug treatment improved all the previous changes and this indicates the protective effect of sulpiride against noise exposure.
Conclusion: it is useful to use sulpiride drug in people who expose to noise stress.
https://ejhm.journals.ekb.eg/article_16409_ffcb0afff7ba94609246863753abe739.pdf
2011-07-01
295
310
10.21608/ejhm.2011.16409
Noise
Sulpiride drug
albino rats
physiological parameters
Histopathalogy and histochemistery
Eman G.E.
Helal
1
Zoology Department ,Faculty of Science,Al-Azhar University ( Physiology)
AUTHOR
Fatma
Eid
2
Zoology Department ,Faculty of Science,Al-Azhar University. ( Histology)
AUTHOR
Neama M
Taha
3
Zoology Department ,Faculty of Science,Al-Azhar University
AUTHOR
ORIGINAL_ARTICLE
Biochemical Markers Of Bone Turnover in Egyptian Women at Different Ages
Osteoporosis is a metabolic bone disease characterized by bone remodeling .This study was done for the detection of some blood and urine markers in cases of osteoporosis among Egyptian women at different ages .The study included 100 women divided into three groups. The first group included 33 women their ages ranged between 35-45 years old, the second included 33 women their ages ranged between 45-55 years old and the third group included 34 women their ages were above 55 years old. Fore all subjects DEXA densitometry was performed. Serum calcium, phosphorous, alkaline phosphatase, Osteocalcin and urinary hydroxyproline, deoxypyridinoline and creatinine were estimated. Results: In osteopenic and osteoporotic women there was elevation in serum alkaline phosphatase and osteocalcin and in urinary pyridinoline and hydroxyproline, while there was decrease in serum calcium .Conclusion: The Egyptian women subjected to the study were suffering from osteopenia from the age of 35 years old or may be earlier Above the age of 55 (postmenopausal) it was found that 50% of women were osteopenic and osteoporotic.
https://ejhm.journals.ekb.eg/article_16410_5f848238657045bef9fef1706092e77b.pdf
2011-07-01
311
317
10.21608/ejhm.2011.16410
Olfat A
Khalil
1
Department of Biochemistry Faculty of Science (Girls) King Abdul Aziz University Jeddah KSA
AUTHOR
Kholoud S
Ramadan
2
Department of Biochemistry Faculty of Science (Girls) King Abdul Aziz University Jeddah KSA
AUTHOR
ORIGINAL_ARTICLE
Adiponectin and interlukin-10 in patients with liver diseases before and after liver transplantation
Background/Aims: Hepatitis C virus (HCV) infection is a global blood-borne disease with the highest prevalence in Egypt. This study was carried out to evaluate the prevalence of adiponectin, Interleukin-10 levels in liver diseases and to identify the potential role of adipocyte derived adiponectin in patients with end stage before and after liver transplantation.
Subject & method: The study population included 69 subjects classified as follow: Control group (G1) healthy individuals, group (G2) Anti HCV +ve & HCV RNA +ve by PCR, group (G3) Anti-HCV +ve &HCV RNA–ve by PCR and liver transplantation group (G4) patients underwent orthotropic liver transplantation; before liver transplantation (G4b) and after one week of liver transplantation (G4a).
Result: Adiponectin levels showed highly significant decrease in G2 (P <0.002), G3 showed insignificant decrease (P<0.6), G4b showed highly significant increased (P <0.0001) and G4a showed insignificant decrease (P<0.08) compared with control group G1. IL-10 levels showed highly significant increase in patients groups (G2, G3, G4b and G4a) as compared with control group (P<0.0001).
Conclusion: Adiponectin and IL-10 may be used as biomarker and/or a mediator of inflammatory states in liver diseases in patients with chronic hepatitis C. We suggest that high adiponectin and IL-10 levels in chronic liver disease before liver transplantation might reflect one of the body’s anti-inflammatory mechanisms in chronic liver diseases.
https://ejhm.journals.ekb.eg/article_16411_375f254e0f2a61a27f0f0b8b62723b3e.pdf
2011-07-01
318
324
10.21608/ejhm.2011.16411
Chronic hepatitis C
Hepatitis C virus
adiponectin
Interleukin-10
Liver transplantation
Abdelgawad A.
Fahmi
1
Determents of Organic Chemistry Faculty of Science, Cairo University Egypt.
AUTHOR
Mohamed A.
El-Desouky
2
Biochemistry Faculty of Science, Cairo University Egypt.
LEAD_AUTHOR
Mohamed M.
El Gamal
3
Determents of Immunology and Microbiology, Military Medical Academy Egypt.
AUTHOR
Mohamed M.
El Gamal
azza_icwater@yahoo.com
4
Determents of Immunology and Microbiology, Military Medical Academy Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
Structural and laboratory changes in the liver of female albino rats in cases of experimental high fat diet and curative role of some medicinal plants.
High fat diets as well as hyperlipidemia represent an important clinical and social problem. It is referred to increased concentration of lipids (Triglycerides, Cholesterol and Fatty acids) in the blood. Such increase may lead to metabolic risks affecting blood vessels and paranchymatous organ mainly the liver. Material and methods: Forty five adult female albino rats were used and divided into 9 equal groups. The first group was considered as a control group. The second group was of high fat diet (25% fat and 2% cholesterol) for 3 weeks. Groups 3,4 and 5 were similar to the second group but received three medicinal plants respectively Oat ,Fennel and Triphala. The sixth group was also of high fat diet but for 6 weeks. Groups 7, 8 and 9 were treated with same medicinal plants as groups 3, 4 and 5. Two main parameters were performed; the first was microscopic study of the liver tissue while the second was laboratory evaluation of liver functions. Results: The hepatic tissue greatly affected by the induction of high fat diet in the form of variable grades of fatty infiltration and vascular congestion either after 3 or 6 weeks of induction. Fibrous content and PAS +ve material were also affected. Structural changes were confirmed by laboratory data. Conclusions: Medicinal plants and regulation of diet quality plays a good role in limiting the risk of fatty liver and atherosclerosis.
https://ejhm.journals.ekb.eg/article_16412_0f84f7d44f9d1223f9a6dc1eecab310f.pdf
2011-07-01
325
343
10.21608/ejhm.2011.16412
High fat diet
medicinal plants
albino rat
Structural and Laboratory findings
Ezz-Eldin E.
Abdalla
1
Histology Departments, Faculty of Medicine Al-Azhar University
AUTHOR
Gamal S.
Elgharabawi
2
Histology Departments, Faculty of Medicine Al-Azhar University
AUTHOR
Moustafa E.
Elsawy
3
Histology Departments, Faculty of Medicine Al-Azhar University
AUTHOR
ORIGINAL_ARTICLE
LigaSure versus Conventional Suture Ligature for Vaginal Hysterectomy: a Randomized Controlled Trial
Introduction: Vaginal hysterectomy is considered to be the method of choice for removal of the uterus. Of particular concern for the vaginal surgeon is the ability to access, visualize, and legate structures while maintaining adequate hemostasis. Surgical hemostasis can be secured by a variety of methods, including mechanical means (sutures) or vessel coagulation (diathermy). Electro-surgical vessel sealing (LigaSure) is a new hemostatic system based on the combination of pressure and bipolar electrical energy and is able to seal vessels up to 7 mm in diameter.
Objective: To assess the safety and efficacy of using the LigaSure vessel sealing system for securing the pedicles during vaginal hysterectomy in comparison with the conventional method of securing the pedicles by suture ligation.
Design: Prospective randomized controlled trial.
Setting: Obstetrics and Gynecology departments (Al-Kharj University Hospital- KSA, Enjab Hospital - UAE and Gulf Medical College and Research Centre- UAE).
Methods: 80 patients undergoing vaginal hysterectomy for benign conditions were randomized to either LigaSure group (n=40) or Suture group (n=40).
Main outcome measures: The primary outcome measures were the operative time and blood loss while the secondary outcome measures were the hospital stay and intra- and post-operative complications.
Results: Patients in the LigaSure group had a significantly reduced operating time (37.1 ± 8.9 min vs. 63.8 ± 10.9 min; P < 0.001), operative blood loss (125.5 ± 33.2 mL vs. 264.6 ± 70.4 mL; P < 0.001), requirement of surgical sutures(1.2 ± 0.4 units vs. 8.2 ± 0.4 units; p < 0.001), pain status (2.0 ± 0.6 vs. 3.7 ± 0.7; P < 0.001), and hospital stay (30.3 ± 2.5 h vs. 45.7 ±10.5 h; P < 0.001) compared to the control group. The overall complication rate in the study was 10 % (8/80), and did not differ between patients of the LigaSure and control group.
Conclusion: The use of LigaSure device can reduce operative time. It allows faster, safe and effective hemostasis compared with the conventional suture ligature. It also reduces the operative blood loss, pain status and hospital stays.
https://ejhm.journals.ekb.eg/article_16413_7e40299e7baa7d21c7f1f0ee3d8f0543.pdf
2011-07-01
344
353
10.21608/ejhm.2011.16413
LigaSure (Electro-surgical vessel sealing)
Mohamed
Abd Elzaher
1
(Al-Azhar University, Cairo, Egypt)
AUTHOR
Ashraf
Moawad
2
Al-Kharj University Hospital (KSA),
AUTHOR
Hanaa
Abu-Ria
3
Gulf Medical College and Research Centre (UAE).
AUTHOR
ORIGINAL_ARTICLE
Modulation of the Pharmacological and Biochemical Actions of Leiurus quinquestriatus (L.q) Scorpion Venom by Exposure to Gamma Radiation
Back ground
This study was undertaken to evaluate the effect of gamma radiation (1.5 KGy & 3 KGy) on L.q scorpion venom. This was carried out by studying the toxicological, biochemical & immunological properties of the venom before and after exposure to gamma radiation.
Material and methods
Animals, venom, antivenin, gamma radiation, 125I.
Results
Data revealed that the toxicity of irradiated venom (1.5 KGy & 3 KGy) decreased as compared to that of the native one. LD50 of irradiated venom were 3.5 mg/kg & 7.5 mg/kg respectively while, that of the native venom was (0.39 mg/kg). Moreover, the distribution of 125I-labeled L.q venom was studied in male Swiss mice tissue using chloramine-T method by being injected intravenously. At various time intervals, urine and blood were collected and the animals were sacrificed. Brain, lungs, heart, liver, kidneys, spleen, intestine, bone and muscle were isolated in order to determine the radioactivity content. The highest contents of 125I-labeled L.q venom were found in the liver and kidney that were quickly excreted into the urinary tract. Trial to label irradiated (1.5 & 3 KGy) L.q venom was unsuccessful due to its decomposition. For that reason the utilization of the labeled irradiated L.q venom is of no meaning. In immunodiffusion technique, both irradiated and native venoms exhibited prominent precipitin bands indicating high concentration of specific antibodies against polyvalent antivenin with venom. Furthermore, the effect of half the LD50 of native or irradiated (1.5 KGy) L.q venom was studied on the activities of certain enzymes: ASAT, ALAT, LDH, CPK, CPK-MB, and the levels of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol and the oxidative stress biomarkers (plasma MDA & blood GSH). Biochemical measurements showed that scorpion venom envenomation caused significant (p < 0.05) elevation in serum ASAT, ALAT, LDH, CPK as well as CPK-MB activities, blood GSH level while, caused significant (p < 0.05) reduction in triglyceride, HDL-cholesterol. In contrast, the 1.5 KGy gamma irradiated L.q venom showed no alterations except in HDL-cholesterol that significantly decreased compared to that of the non-envenomated normal rats.
Conclusion
These results indicated that 1.5 KGy gamma irradiation of venom offer an effective method for reducing the toxic effect of venom.
https://ejhm.journals.ekb.eg/article_16414_1815ac0d94a2e123aadeb7690d374d2e.pdf
2011-07-01
354
370
10.21608/ejhm.2011.16414
gamma radiation
Leiurus quinquestriatus scorpion venom (L.q)
LD50
125I-labeled L.q
Double immunodiffusion
liver and cardiac enzymes
Lipid profile
oxidative stress biomarkers
Heba A.
Mohamed
1
Department of drug radiation research – National Center for Radiation Research and Technology
AUTHOR
Esmat A.
Shaaban
2
Department of drug radiation research – National Center for Radiation Research and Technology
AUTHOR
Aber M
Amin
3
Department of labeled compound – Hot Lab Center – Atomic Energy Authority
AUTHOR
Sanaa A.
Kenawy
4
Department of Pharmacology and Toxicology – Faculty of Pharmacy- Cairo University
AUTHOR
ORIGINAL_ARTICLE
Electroencephalogram in Childhood Neurological Disorders
Aim of the study: The present study was done to obtain a baseline for clinical indications of EEG in children and to evaluate the Electroencephalography (EEG) findings in children with various acute, chronic CNS disorders and non epileptic events.
Patients and methods: The Electroencephalography (EEG) records of 250 patients were studied, which was done at neurophysiology departments of Queen Rania AL-Abdullah Hospital for children in Jordan. For each patient underwent EEG record the following data were recorded, age, sex, source of referral ( in patient department or out patient ) , reason for Electroencephalography (EEG) , diagnostic impressions and clinical presentation , the result of the EEG examinations and clinical correlation between the seizure type and EEG finding .
Results: Males slightly outnumbered females: 55% males. The majority of cases sent for EEG fell between 6-12 years , Total of 63.2 % of all referrals for EEG were from outpatient clinic while in patients account for 36.8%. pediatric neurology department referrals being highest 28.8 %, the majority of diagnosis at referral were suspected epilepsy (80%), epileptiform EEG abnormalities was detected in ( 32%) , Over all 64 % the EEG records were normal , All EEG records of children with syncope , headache were normal .
Conclusion and recommendation: We conclude that there are many unnecessary routine EEG recordings in children; Investigation of epilepsy and acute encephalopathies appear to be the most valuable indications for routine pediatric EEG. EEG can help in classified of the seizure, finding a way to reduce EEG requests is request
https://ejhm.journals.ekb.eg/article_16415_b2c3f06d860d15807de5a75365f3164f.pdf
2011-07-01
371
379
10.21608/ejhm.2011.16415
Epilepsy
Electroencephalography
paroxysmal event
Wael Hayel
Khreisat
1
Queen Rania al Abdullah Hospital for Children King Hussein Medical Center Royal Medical Services Amman. Jordan
AUTHOR
ORIGINAL_ARTICLE
Protective role of Peanut oil in rats exposed to two different doses of gamma radiation that produced oxidative stress and bone injury
Introduction: Exposure to ionizing radiation represents a genuine, increasing threat to mankind and our environment. The steadily increasing applications of radiation in clinical practice, industrial and agricultural activities, on top of residual radioactivity resulting from nuclear test explosions, have a measurable impact contributing to possible radiation hazards in humans. Control of radiation hazards is considered as one of the most important challenges in order to protect our lives from radiation damages.
The trans-3,4,5-trihydroxystibeneis a phyto-chemical present in peanuts and grapes with beneficial effects such as protection against cardiovascular disease and cancer prevention.
Purpose: The present study aims to clarify the role of peanut oil as a radioprotector in male albino rats against oxidative stress and bone injury induced by two different doses of gamma irradiation.
Material and Methods: Rats were subjected to a dose of 5 Gy (group 3) or 10 Gy ( group 4 ) ( single dose/ whole body ) in comparison with control group ( group 1 ). Prior to the two doses of gamma radiation, rats received peanut oil subcutaneously, ( 0.75 ml/kg ) over one month period, on three days/week ( group 5 and 6 ). Group 2, rats received peanut oil subcutaneously, ( 0.75 ml/kg ) as group 5, but without exposure to radiation.
Results: The results showed that whole body gamma irradiation revealed significant acceleration in the level of lipid peroxide (MDA), with significant depletion in glutathione content (GSH), superoxide dismutase (SOD) and catalase (CAT) activities. Meanwhile. Also, the study showed significant increase in serum calcium with concomitant decrease in the bone calcium and significant increase in serum inorganic phosphorous concomitant with a decrease in bone phosphorous after radiation exposure.
Administration of peanut oil pre-irradiation has significantly ameliorated the radiation induced disturbance in all the investigated parameters.
Conclusion: Metabolism can be controlled to some extent by peanut oil administrated prior to irradiation.
https://ejhm.journals.ekb.eg/article_16416_1c1f5d92f0dfcd19c39601e307f052d8.pdf
2011-07-01
380
391
10.21608/ejhm.2011.16416
Peanut oil
Ionizing radiation
Antioxidants – Calcium
phosphorous
Tamer M. M.
Saad
1
Medical and Radiation Research Department, Nuclear Materials Authority,Cairo, Egypt
AUTHOR
Amal A. A.
Ammar
2
Medical and Radiation Research Department, Nuclear Materials Authority,Cairo, Egypt
AUTHOR
ORIGINAL_ARTICLE
Protection Against Schistosoma Mansoni In Mice By using UV-Irradiation In Comparing With Soluble Adult Worm Antigen (SWAP)
Background: The present study aimed to investigate the dynamics of immunoregulatory factors implicated in the murine model of soluble adult worm antigen preparation (SWAP)and UV-irradiated adult worm extract-induced granulomatous hypo-responsivness. Material and Methods: Native male mice were classified into four groups. The first negative control (non-infected) group, the second positive control (infected) group, the third SWAP-vaccinated group and the fourth UV-irradiated adult worm extract-vaccinated group. The last two groups were injected subcutaneous by 0.2 ml (contained 100 µg protein) of the two used vaccines SWAP or UV-irradiated adult worm extract respectively, four weeks prior to infection. The 2nd, 3rd and 4th mice groups were infected by subcutaneous injection with 100 cercariae of Egyptian strain of S. mansoni. All mice groups were sacrificed 8 weeks post-infection. Results:Parasitological parameters such as worms count, eggs/gram tissues of liver and intestine were studied. Biochemical parameters such as the activities of liver function tests (AST, ALT, ALP and γGT) were measured. The cellular immune responses were assessed by studying the cytokine measurement. Hepatic histopathological criteria and the morphological changes of adult worms were studied through the scanning electron microscope. Conclusion: The present results revealed that the UV-irradiated adult worm extract have high efficacy than SWAP as immunizing antigens as shows in our data, which reported that irradiation associated vaccine antigen was shown to achieve a higher protection in mice.
https://ejhm.journals.ekb.eg/article_16417_c6cca5dd93f90657ead26cacf229edcc.pdf
2011-07-01
392
402
10.21608/ejhm.2011.16417
Schistosomes-Radiation-Liver enzymes
M.M.S
Tamer
1
Medical and Radiation Research Dept., Nuclear Material Authority
AUTHOR
A.A.A
Amal
2
Medical and Radiation Research Dept., Nuclear Material Authority
AUTHOR
K.A.S.
Hazem
3
Medical and Radiation Research Dept., Nuclear Material Authority
AUTHOR