Bakr, K., Abd Al-Rahman, M., Abdou, A., Al-Tamimy, H. (2019). Detection of Some Genetic Polymorphism and Serological Factors Associated with Rheumatoid Arthritis in Egyptian Patients. The Egyptian Journal of Hospital Medicine, 77(1), 4777-4784. doi: 10.21608/ejhm.2019.46760
Kareman M. Bakr; Maha M. Abd Al-Rahman; Aml E. Abdou; Hegazy M. Al-Tamimy. "Detection of Some Genetic Polymorphism and Serological Factors Associated with Rheumatoid Arthritis in Egyptian Patients". The Egyptian Journal of Hospital Medicine, 77, 1, 2019, 4777-4784. doi: 10.21608/ejhm.2019.46760
Bakr, K., Abd Al-Rahman, M., Abdou, A., Al-Tamimy, H. (2019). 'Detection of Some Genetic Polymorphism and Serological Factors Associated with Rheumatoid Arthritis in Egyptian Patients', The Egyptian Journal of Hospital Medicine, 77(1), pp. 4777-4784. doi: 10.21608/ejhm.2019.46760
Bakr, K., Abd Al-Rahman, M., Abdou, A., Al-Tamimy, H. Detection of Some Genetic Polymorphism and Serological Factors Associated with Rheumatoid Arthritis in Egyptian Patients. The Egyptian Journal of Hospital Medicine, 2019; 77(1): 4777-4784. doi: 10.21608/ejhm.2019.46760
Detection of Some Genetic Polymorphism and Serological Factors Associated with Rheumatoid Arthritis in Egyptian Patients
1Department of Microbiology& Immunology, Faculty of Pharmacy (Girls), AL-Azhar University
2Department of Microbiology, Faculty of Medicine (Girls), AL-Azhar University
3Department of Rheumatology, Physical Medicine & Rehabilitation, Faculty of Medicine (boys), AL-Azhar University, Cairo, Egypt
Abstract
Background: Rheumatoid arthritis is a chronic inflammatory autoimmune disease thatis characterized by destruction of cartilage and bone. PADI4 gene encoding the peptidyl arginine deiminase 4 citrullinating enzyme that has been found to be over expressed in rheumatoid arthritis patients. Objective: To investigate relation between PADI4 gene polymorphisms and anti–cyclic citrullinated peptide (Anti-CCP) autoantibody levels in sera of Egyptian rheumatoid arthritis patients, and to find its relevance to the disease activity(DAS28 score) and other inflammatory markers as Rheumatoid factor (RF). Methods: PADI4-89 andPADI4 -92 single nucleotide polymorphisms (SNPs) were analyzed in thirty RA patients and twenty healthy controls by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. Serum anti-CCP levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: PADI4-89 genotype A/G was present in RA patients at higher frequency compared to controls (P = 0.024). (G) allele frequency was significantly higher in RA patients than in controls (P= 0.047). Level of Anti – CCP was significantly higher in RA patients serum than in controls (P= 0.000).PADI4-89 A/G + G/G genotype and PADI4-92 C/G+GG genotype were associated with higher DAS28 score, Rheumatoid factor (RF), and anti-cyclic citrullinated protein (Anti – CCP). Significant positive correlation was found between serum level of Anti– CCP and DAS28 score (P= 0.023). Conclusion: The SNPs A/G and G/G of PADI4-89 were associated with RA susceptibility. Relation was found between both PADI4 polymorphism and anti-CCP levels.