2024-03-29T10:49:59Z
https://ejhm.journals.ekb.eg/?_action=export&rf=summon&issue=3766
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
The role of infection in irritable bowel syndrome
Hani
Abu Zeid
Magdi
Abdel Karim
Mahmoud M.
Bazid
Mostafa M.
Al Sayed
Mostafa Yousef
El- Mishad
A clinically distinct subset of Irritable Bowel syndrome (IBS) could be induced or exaggerated by bowel infection that is called post-infective IBS (PI-IBS) which is characterized by diarrhea predominant symptoms with less psychiatric illness. The purpose of this work was to study whether some bowel infections contribute to the pathogenesis of IBS and whether the treatment of such infections reduces IBS symptoms.
100 patients with symptoms suggestive of IBS according to Rome II Criteria were chosen from Internal Medicine outpatient clinic-Al Hussein university hospital. They were 64 females and 36 males, aged 18-52 years old with mean age 38,2 3,6 years. They were submitted to: full clinical examination and frequent stool examinations (direct smear and culture) before, soon after and six months after administration of drug therapies for common stool-positive associated bowel infections (diloxanide for E. histolytica, metronidazole for Giardia lamblia, Ketoconazole for Candida, ciprofloxacin for Salmonella and neomycin for Pseudomonas).
Results obtained showed that 24% of patients had diarrhea predominant symptoms, 13 patients (54%) out of them had history suggestive of acute gastroenteritis.
80 patients had initial positive stool examination and continued the post treatment six months follow up study. Out of them, 13 patients had stool positive for Salmonella typhi mixed with E. histolytica; ten of them (76.9%) showed a statistically significant persistent clinical and laboratory improvement after ciprofloxacin therapy (P < 0.01). So Salmonella typhi may be implicated in IBS pathogenesis.
Six patients showed isolated Giardia lamblia infection; four of them (66.7%) showed a statistically significant persistent clinical and laboratory improvement after metronidazole thereby (P < 0.05). Giardia lamblia parasite may be considered in triggering or exaggerating IBS symptoms.
24 patients had isolated E. histolytica infection; only one of them (4.2%) showed sustained clinical and Laboratory improvement after diloxanide therapy -statistically insignificant result- (P > 0.05). This finding makes the correlation between E. histolytica infection and IBS is unlikely.
20 patients had isolated Candida overgrowth on stool examination; eight of them (40%) showed a statistically significant clinical and laboratory improvement after Ketoconazole therapy (P < 0.05). Therefore Candida overgrowth may induce or exaggerate IBS symptoms.
Finally, no growth was noticed for shigella sp. or E. coli, this may exclude the role of such organisms in IBS pathogenesis.
2005
01
01
1
7
https://ejhm.journals.ekb.eg/article_18136_e5a8e507a2fde01967d3de015dc7d331.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Analysis of bone density of the human first metatarsal bone
Nabil
Amin
Abd El Hakim
Rozeek
Naglaa
Dabees
This study was performed on 38 Pairs of cadaveric human 1st metatarsal bones in an attempt to establish the pattern of bone mineral density and to correlate it with the biomechanical function of the bone. The results show that the head is denser than the base, the dorsal portion of the whole metatarsal is denser than the planter portion and the lateral portion of the whole metatarsal is denser than the planter portion and the lateral portion of the whole metatarsal is denser than the medial aspect. The same pattern of bone density with respect to dorsal vs planter and Lateral vs medial was also seen in the head and compared with same portions of the metatarsal as a whole. The relation-ship between the bone density distribution of the 1st metatarsal bone and their biomechanical function in the gait cycle was discussed.
2005
01
01
8
15
https://ejhm.journals.ekb.eg/article_18137_ae846ad7378147b199b78af1767d5fec.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Protective Effect Of Ascorbic Acid On Cisplatin Genotoxicity In Male Mice Bone Marrow Cells
Nada, H.A.
Al- Twaty
Cisplatin (cis-diamminedichloroplatinum II) is an effective antitumor agent with a wide spectrum of activity against varies solid tumors, but it has serious side effects on nontumour cells. Cisplatin produces intra- and interstrand DNA cross-linking effects and chromosomal aberrations in mammalian cells. Vitamin C (ascorbic acid) is an antioxidant that can scavenge free radicals and protect cellular macromolecules, including DNA, from oxidative damage induced by different agents. Pretreatment administration of ascorbic acid on cisplatin induced chromosome aberrations has been determined in bone marrow cells of Swiss albino mice. Results showed that cisplatin (7.5 & 10mg/kg bw)IP injection to male mice induced significant increase in the frequencies of chromosomal aberrations. The results of pre-treatment with ascorbic acid (66mg/kg bw) showed a significant decrease in the number of chromosomal aberrations induced with cisplatin tested doses. Ascorbic acid did not exhibit any clastogenic effect in male mice bone marrow cells. We concluded that ascorbic acid has a protective role against the genotoxicity induced by antitumor drug cisplatin.
Cisplatin- Antioxidants- Ascorbic Acid-Chromosomal Aberration- genotoxicity
Bone Marrow Cells- Mice
2005
01
01
16
22
https://ejhm.journals.ekb.eg/article_18138_de23d8f6083a1fede809fd63116bf549.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Clastogenic Effect of Azadirachtin of Neemix-4.5 On SWR/J Mouse Bone Marrow Cells
F. M.
Abou-Tarboush
H. M.
El-Ashmaoui
H. I.
Hussein
D.
Al-Rajhy
M.
Al-Assiry
The clastogenic effect of azadirachtin of neemix-4.5 was investigated in SWR/J mouse bone marrow cells. Males and females, 5 each per treatment time, aged 10–12 weeks and weighing 31.7–33.8 g, were orally administrated 9.0 mg/kg (1/10 LD50) of azadirachtin solution. A control group (5 males and 5 females) received only sterile distilled water. The animals were sacrificed 6, 12, 24, 48 or 72 h post- treatment. The chromosome preparations were obtained from bone marrow cells. Chromatid and chromosomal aberrations were investigated in 50 metaphases per animal.
No significant differences in the frequency of chromosomal aberrations or in the percentage of mitotic index were observed between the treated male and female mice at any time intervals used. Hence, data from the two sexes were pooled when analyzed statistically. In the prersent study, the dose level 9.0 mg/kg body weight of azadirachtin of neemix-4.5 did not induce any significant (p>0.05) changes in the percentages of mitotic indices or in chromosomal aberrations in the bone marrow cells of treated animals at all time intervals tested compared with the control group. As the pharmacokinetics of azadirachtin is unknown, the essentially negative results in the present study may be due to a lack of genotoxic potential .
Neemix-4.5
Azadirachtin
Clastogenic effect
Bone Marrow Cells
Mice
2005
01
01
23
28
https://ejhm.journals.ekb.eg/article_18139_a5d37f8851ef1522f246811dfa3dc337.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Survey For Detection and Determination of Aflatoxins M1 and B1 in local Milk and Certain Dairy Products by Thin Layer Chromatographic Method.
T.A.
Nassib
S.N.
Guergues
M.M.
Motawee
90 different type of milk samples, 10 Yogurt Samples, 110 different type of cheese samples and 10 ice cream samples were collected randomly from Giza Governorate during the summer of 1998 – 1999, for detection and determination of Aflatoxins M1& B1 by using thin layer chromatographic method. Results revealed that the average range of Aflatoxin M1 in milk samples amounted from 0.144 to 0.378 ng/ml. About 20 % of cows and buffaloes milk samples contained form 0.378 to 0.342 ng/ml of AFM1, whereas about 10% of other milk samples were contaminated with 0.162, 0.288, 0.324, 0.234, 0.144 and 0.162 ng/ml for skim , Pasteurized , sterilized, UHT, powder, and baby milk, in the same order. Concentrations of AFM1 detected in cheese samples, furthermore, varied due to the type and age of cheese being examined. 20% of cheese samples were contaminated with AFM1 being 5.1, 3.2, 2.99, 2.099, and 2.34 ng/gm for fresh Domiati, aged Domiati, Processed and Karish cheese, respectively, whereas, 30% of the other types of cheese contained 5.88, 6.3 and 3.4 ng/gm for Roquefort, fresh Romi, and Cheddar cheese, respectively. The lowest concentration of AFM1, of 0.116 ng/gm was detected, however, in 10% of yogurt samples. Meanwhile, 20% of ice cream samples were found to be contaminated with 2.7 ng/ml, and 10% of Feta cheese samples contained 3.3 ng/gm. It could also be appeared from results that both of cream and spread cheese were found completely free from this Aflatoxin, the lowest content of Aflatoxin detected in all of the above examined samples was 0.116, 0.162, 0.162 and 0.216 (ppb) in yogurt, skim, baby milk and cream, respectively. On the other hand, results also indicated that all milk samples were free from Aflatoxin B1 except one sample of skim milk (out of 10) which gave positive result.
2005
01
01
29
36
https://ejhm.journals.ekb.eg/article_18140_c0edfe01500c935e2e7fe9aeb7fc9e16.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Study of the single and combined genotoxic effects of chlorpyrifos and quercetin in Saccharomyces cereviciae
Nada, H. A.
Al-Twaty
The genotoxic effects of chlorpyrifos and quercetin either single or combined were tested in terms of their ability to induce reverse mutation, gene conversion and mitotic crossing over in Saccharomyces cereviciae D7. The results indicated that all single and combined treatments induced reverse mutation, gene conversion and mitotic crossing over in Saccharomyces cereviciae D7. Combined treatment was more effective than the single treatment of quercetin. The insecticide (chlorpyrifos and quercetin which are common flavonoids) proved to be mutageneic in Saccharomyces cereviciae.
2005
01
01
37
42
https://ejhm.journals.ekb.eg/article_18141_957432feeb268714d93fe630bd51487d.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Comparison between some different methods for determination of Aflatoxin M1 in milk and some diary products
T.A.
Nassib
S.N.
Guergues
M.M.
Motawee
Seven TLC methods for evaluate the incidence of Aflatoxin M1 in milk and dairy products were used, Pons et al. (1973); Fukayama et al. (1980); Stubblefield (1979); Official Method of Analysis (1995); Van Egmond and Stubblefield (1981); Official Method of Analysis (1984) and Official Method of Analysis (1990) , to detect the sensitive method among them for determination of Aflatoxin M1 (AFM1). Results show that, the Official Method of Analysis, 1995 (AOAC, 1995) was the sensitive method for determination of AFM1 among the all tested methods, where it gives the highest recovery percentage for liquid milk, Cheese, and Powdered milk, 106.2%, 95.99% and 104.4% respectively. While Stubblefield, (1979) method gave about 75% AFM1 recovery for Yogurt, but when we made a slight modification on it gave 92% AFM1 recovery.
2005
01
01
43
47
https://ejhm.journals.ekb.eg/article_18142_6e7514034bb63eb8d4332dfa30c83fe5.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Response Of Mice Tissues To Intranasal Bacterial Lysate Vaccination: Immunohistopathological Study
Hanaa A.
Mansour
Effective protection against pathogenic bacteria requires both mucosal and systemic immune responses. Intranasal administration of antigens induces these responses. The protective effect of intranasal immunization with different formalin-inactivated bacterial lysates in albino mice was evaluated. This study used formalin inactivated lysate of each of the following Escherichia coli , Pseudomonas aeroginosa ,Salmonella typhi , Shigella flexeneri , Staphylococcus aureus , Bacillus subtilis and mixed bacterial lysates. The lysate was administered intranasally (5μl/ nostril) once daily for seven consecutive days. The results of this study recorded some changes in peripheral blood total and differential leucocytic counts , peritoneal fluid and bone marrow lymphocytic percentages . Spleen and thymus weight changes were reported under the effect of Salmonella typhi lysate, Shigella flexeneri lysate and Staph. aureus lysate. The level of immunoglobulin G (Ig G) was assessed in serum, bronchial lavage and nasal bed harvest. The levels of Ig G were significantly elevated in the three determinants, suggesting an efficient immunostimulatory effect of bacterial lysates. Some of these levels were exceeding 2-3 folds of that of the control group. Histopathological studies recorded changes in some reticuloendothelial system organs including the liver, spleen and thymus gland, besides, some changes were also observed in the lung and bronchi under the effect of intranasal vaccination. This study supports the immunoprotective effect of intranasal vaccination, using bacterial lysates.
2005
01
01
48
65
https://ejhm.journals.ekb.eg/article_18143_cd6ff8a3c571e16d2283b0e42e5ba434.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Ambivalent property of bilirubin in human bile juice
Anna
Blázovics
Péter
Sípos
Ferenc
Örsi
Mervat
Abdel Rahman
Gallstones are formed as a result of many metabolic disorders e.g. chronic haemolytic anaemia, diabetes mellitus, ileal diseases, short bowel syndrome, gluten sensitive enteropathy, elevated serum lipids or Crohn’s disease. The relationship between gallstone disease and free radical reactions is not known exactly even today. Free radicals are involved in many clinical conditions e.g. in hyperlipidemia and in fatty liver. Oxygen free radicals are produced and accumulated while the function of mitochondrial and microsomal electron transport or in peroxisomes and the activated arachidonic acid cascade. Spontaneous lipid peroxidation and oxygen free radical products of respiratory burst of Kupffer cells can be added to peroxide pool of liver tissue. Tissues, cells and subcellular particles exhibit different specific defence activities in pathological processes, which involve free radicals.
The activity of microsomal P450 enzyme system and the microsomal structure are changed during pathological free radical attack and the cholesterol/bile acid ratio in bile juice is also altered. At the same time bilirubin metabolism can also be modified. Bile samples of 88 cholecystectomysed patients in both sexes (male: 29, female: 59) were examined. HPLC analysis (HP1090 liquid chromatograph with diode array detector) was used for the detection of free bilirubin and bilirubin derivates. HP5890 gas chromatograph and flame ionization detector was used for fatty acid analysis. The induced chemiluminescence intensity was also determined in bile juice with (Berthold Lumat 9501) luminometer.
As results show, the occurrence of C18:1 9, C18:2 6, C20:4 6 fatty acids were in high percentage in gallbladder bile in every case of randomly chosen 17 cholecystectomysed patients in both sexes suffered from cholecystitis chronica with gallstone. Lipid peroxidation products (diene conjugates and malondialdehyde) were detected in all cases of bile as well. Mathematical statistical analysis showed, that positive significant correlation was between low concentration of total bilirubin of gallbladder bile and chemiluminescent intensity in hydrogen peroxide - luminol system. Extra high chemiluminescence light could be detected in bile samples of patients with severe clinical state. Bilirubin pro-and antioxidant forms are justified in human gallbladder bile.
Bilirubin
human bile
gallbladder
gallstones
Free radicals
2005
01
01
66
72
https://ejhm.journals.ekb.eg/article_18144_4889ba1f58465dfba4addccb2a685b9a.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Female Breast Cancer: Epidemiological And Clinical Study Of Some Risk Factors Among Egyptian Females- Multi Clinics Study
E. A.
El-Moselhy
M. A. S.
Ahmed
A. M.
Abdel-Fattah
A. Y.
Mohammed
T. A.
Selmy
H. M.
Sakr
A.
Abusrie
M. A.
Fahmy
A. S.
Salem
This study was conducted on 390 female breast cancer patients and an equal number of females as controls. The patients were attending some University and Teaching Hospitals in Cairo and Assuit. Ninety of them were newly operated. A retrospective, case-control, clinic based study was chosen to carry out this research. The aim of the study was to describe the sociodemographic, characteristics and clinical features of female breast cancer and to determine its risk factors among Egyptian women. All the patients and the controls had undergone physical examinations. Laboratory investigations were done for the newly operated patients and their controls. The most important characteristics of breast cancer patients were breast mass as the main presenting symptom (92.1%) and treated by modified radical mastectomy (77.2%). Low means level of serum vitamin D and high levels of serum cholesterol and triglyceride were found more among the patients. Age at first full term pregnancy ≥ 30 years, age at menopause ≥45 years, pregnancy termination and/or abortion and never married were important gynaecological and reproductive risk factors (OR=4.44, 3.14, 2.84 and 2.67, respectively). Also, exposure to radiation and/or environmental factors, history of benign breast disease and alcohol use were important associated risk factors (OR=5.05, 4.63 and 4.10, respectively). Moreover, the sister as the nearest female relative with breast cancer, total number of female relatives with breast cancer ≥2 and relative(s) age at diagnosis <50 years were important family history risk factors (OR=9.19, 8.84 and 7.91, respectively). Lastly, high consumption of canned foods, fat rich foods and low consumption of fresh fruits and vegetables were important dietary risk factors (OR=3.39, 1.76 and 1.51, respectively).
2005
01
01
73
87
https://ejhm.journals.ekb.eg/article_18145_b98b9e81c4b217f54cd7c1c654424a6b.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Modifying effects of Soybean and Nigella Sativa against Experimental Hepato-carcinogenesis induced By Nitrosamine Precursor in Rats. (Histopathological and electron microscopically study)
Hanaa F.
Waer
Abdel Baset
EL Asser
Hamdy A.
Ibrahim
Maher M.
Khalifa
Saad M
Elgendy
Khaled I.
Magrabi
Cancer has become an important topic in medicine since it is a major cause of death in the developing countries and it is now only secondary to that of myocardial infraction. Nitrosamines precursors are known to be carcinogenic to humans, in various organs at relatively low levels of exposure. It induces benign and malignant tumors especially liver tumors following its administration by various routes, including ingestion and inhalation. Humans may be exposed to Nitrosamines through the ingestion of food that contains it, such as cured meat products, and smoked fish. Other exposures to Nitrosamines may be from drinking contaminated water and from breathing cigarette smoke and contaminated ambient air. Individual are most likely to be exposed to Nitrosamines in occupational settings such as in the rubber, tannery, fish processing, dyes, and surfactant industries. The prevalence of liver tumors throughout the world makes in imperative to seek chemo preventive agents.
Vegetables, natural products of plant origin and numerous non-nutritive dietary constituents have been shown to play a salutary role in cancer chemoprevention. The present study aims to evaluate the chemo preventive efficacy of soy bean and Nigella sativa on hepato- carcinogenesis induced by dibuty1 nitrosamine (DEN).
It could be observed that both soy bean and Nigella sativa have a good effect of amelioration against liver hepatoma induced by nitrosamine. Soy bean more or less showed more prerogative effect than Nigella 9 and 12 months after administration.
2005
01
01
88
115
https://ejhm.journals.ekb.eg/article_18146_536b0c76a0fda80a979869c4bbbc04a7.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Effect of food preservative and food coloring agent on some physiological and hematological parameters in albino rats and the protective role of garlic
Eman G.E.
Helal
Mervat
Abdel- Rahman
Most children, in the age of nursery consume food that contains both colorants and preservatives with great amount. This observation led us to study the interaction between sodium nitrite as a food preservative and sun set yellow as a coloring agent. The mixture of the two agents at the limited dose of each was found to be a lethal dose. So, 1/10th of this dose was used daily for 30 days. Animals were divided into three groups. The first group served as a control, while the second group was orally administered a mixture of 10 mg sod.nitrite (NaNO3)/kg mixed with 0.5 mg/kg/day sun set yellow (S.S.Y). The third group received garlic (5 mcg/kg) in addition to the above mentioned mixture. After 30 days of treatment, half of the animals from each group were decapitated. The other half of the animals was left for another 15 days without any additional treatment as a recovery period.
Ingestion of the mixture of (NaNO3 and S.S.Y) significantly decreased rat body weight, RBCs and WBCs counts, Hb%, Hct%. No changes were recorded for organ/ body weight, respiratory rate, heart rate, rectal temperature.
A complete recovery from the abnormalities of most physiological and hematological parameters was observed after the recovery period or when garlic was administered.
Garlic
antioxidant
preservatives
colorants
2005
01
01
116
123
https://ejhm.journals.ekb.eg/article_18147_b8dbfd91ebe5619c0b55af61d7732bf0.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Serological And Haematological Responses To Experimental Fascioliasis And Treatment
Nour El Din H.
Sherif
Ismail M.
Shalaby
Maha F.
Soliman
Mohamed G.
Hassan
This study was established to evaluate and monitor both the humoral and haematological responses to Fascioliasis and its treatment in rabbit model. Rabbits were orally inoculated with viable F. gigantica metacercariae and then divided into four groups. Infected rabbits were orally administered a single dose of Triclabendazole (TCBZ) (10 mg kg-1) at week 4, 8 or 12 post- infection (pi), respectively. Antibody (Ab) response against infection was monitored using enzyme linked immunosorbant assay (ELISA). Total red blood cell counts (Rbcs), haemoglobin content (Hb) and total and differential white blood cells (Wbcs) were also determined. Infected rabbits were found to produce Ab against excretory-secretory products (ESP) of adult flukes two weeks pi where ELISA enabled the early diagnosis of infection. Ab level reached to the peak at week 10 pi. In TCBZ-treated groups, the early Ab responses prior to treatments were similar to response of infected-untreated rabbits. But, after different treatment regimens, Ab levels showed a significant decreases that were depended mainly on both time and hence efficacy of the treatment. In addition, significant reductions in both Rbcs and Hb values accompanied with mild anemia were found among infected group at week 12 &10 pi, respectively. TCBZ-treatment prevented the development of anaemia. Eosinophil numbers significantly increased starting from week 2 pi and peaking at week 4 or 6 pi in all groups. Hence, both eosniophilia and anaemia might be characteristic aspects for experimental fascioliasis.
Fascioliasis
Triclabendazole
enzyme linked immunosorbant assay
Haematology
2005
01
01
124
132
https://ejhm.journals.ekb.eg/article_18148_a76170d4d3ce29e2c654396b242e063d.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Developmental and Histological Studies of Fungiform Papillae in Fetal Human Tongue
Lotfy
Sayed
Atef
Ibrahim
Esam
Abdelhady
Hosam El-Din
Hussin
Ghassan
Ahmed
This study was designed to evaluate the histological development of fungiform papillae of human tongue during the prenatal period.
Lateral Sagittal sections were taken at autopsy from the anterior two-third of 18 aborted fetuses of 8, 10, 12, and 20 weeks of gestations and 2 stillborn infants at 40 weeks of gestation. Twenty specimens were prepared in paraffin and stained for histological study. The gestational ages were calculated after measuring the Crown Rump Length [C.R.L.]. It was found that the first appearance of fungiform papillae was nearly about the 10th week of gestation, before the appearance of filiform papillae, most of the developed fungiform papillae were cylindrical in shaped with appearance of nerve plexus, at 12th week they reached mushroom shaped with narrow base and broad apex and became differentiated into primary and secondary papillae, also presumptive taste buds were noticed at this age in the form of multiple local thickening of papillary epithelium. At 20th week of gestation the taste buds became more developed, the papillae appeared more vascular. At full term (40th week) the fungi form papillae reached full maturity.
It is concluded that this study suggests that the development of fungi form papillae requires an epithelial and mesenchymal interaction during morphogenesis
2005
01
01
133
141
https://ejhm.journals.ekb.eg/article_18149_ec36fe86cb0e0402b64c4d48da3bb8cd.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Histological and Ultrastructural Changes of the Brain, Pancreas and Ileum of diabetic and nondiabetic mice under heat stress with special reference to heat shock proteins.
Hamdy Hamed
Swelim
The current study was designed to evaluate the thermal stress effect on brain, pancreas and ileum in normal and alloxan-diabetic mice by studying the structural changes at both light and electron microscopical levels and by studying the molecular changes expressed through the examination of protein banding pattern in the electrophorograms of the different groups . The animals were divided into four main groups; control mice, alloxan diabetic mice, heat-stressed nondiabetic mice and heat-stressed diabetic mice.
No remarkable changes could be detected in nonstressed diabetic animals except the ultrastructural changes noticed in B cells of islets of Langerhans in pancreas. The heat-stressed nondiabetic animals displayed various cellular and subcellular changes in the organs of study, which were focal in most of the cases. Signs of restitution were also noted in the three selected organs in this group . On the other hand, heat stress was so much destructive in diabetic mice and that was so clear specially in pancreas. The difference in degree of cellular injury between diabetics and nondiabetics is correlated with data of protein studies which demonstrated more expression of heat stress proteins (HSPs) in nondiabetics and attenuation of this expression in diabetics. These stress proteins are suggested to play an important role in protection against thermal stress injury. Consistent with this, the brain which showed more expression of HSPs was the least affected of the three organs. Moreover, the attenuated expression of these HSPs in diabetics highlights the suggestion that diabetes deranged the stress response and delayed the expression of the protective HSPs.
In conclusion further studies are needed to characterize the molecular structure of the HSPs and the genes responsible for the expression of these proteins in these tissues and the other body tissues .
2005
01
01
142
170
https://ejhm.journals.ekb.eg/article_18150_617b24deae70b5b588a35936c105432c.pdf
The Egyptian Journal of Hospital Medicine
1687-2002
1687-2002
2005
18
1
Systemic Correlative Study between Systemic Lupus Erythematosus, Osteoporoses And Dehydroepiandrosterone-S(DHEA-S)level In Premenoposal Egyptian Women
El Shafaey
M
Eman G.E.
Helal
Hala H.
Elsayed
Abeer S.M.
Gawish,
Systemic Lupus Erythematosus (SLE) is an autoimmune disease commonly occurs in of childbearing age, with connective tissue inflammation particularly joints and causes characteristic rashes.
Subjects & Methods: the present study includs30 premenopausal females, they were divided into 3 groups. Group I consists o ten premenopausal females without SLE, Group II include ten premenopausal females had SLE,disease duration less than three years, Group III include ten premenopausal females had SLE,disease duration more than three years. Bone mineral density (BMD) in the heel of right foot by Achilles Express in all groups had been performed. Estimation of the serum level of Dehydroepiandrosterone Sulphate (DHEA-S) hormone and serum level of calcium, phosphorous ,sodium and potassium.
Results: the results of the present study showed that in group II the BMD was 10% with osteoporotic , 40% with osteopenic and 50% with normal BMD, group III the BMD was 10% with osteoporotic , 60% with osteopenic and 30% with normal BMD. The correlations were done between BMD and serum minerals calcium, phosphorous, sodium and potassium in SLE patients ( Group II&III). Statistically high significant increase was found among osteopenic versus control women. Moreover a significant increase of serum calcium and sodium while there was a significant decrease in serum DHEA-S, phosphorous and potassium.
Conclusions: There is a relationship between level of DHEA and the progression of SLE. Moreover there is relation between the decline in serum levels of DHEA-S and phosphorous , and the elevation of serum levels of calcium and the occurrence of osteoporosis in SLE.
Treatment with DHE is beneficial in controlling of the disease activity in LES patients.
2005
01
01
171
185
https://ejhm.journals.ekb.eg/article_18151_06ab24f11f48039562c0d1afbce3476c.pdf