Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Expression of CD38 and CXCR3 in children with atopic dermatitis.
467
478
EN
Fawzia A.
EL.Sheshtawy
Departments of Clinical Pathology, Dermatology , Pediatric # , Faculty of Medicine for Girls- Al Azhar University
Naglaa A .
Ahmad
Departments of Clinical Pathology, Dermatology , Pediatric # , Faculty of Medicine for Girls- Al Azhar University
Manal
Abdul Salam
Departments of Clinical Pathology, Dermatology , Pediatric # , Faculty of Medicine for Girls- Al Azhar University
10.21608/ejhm.2008.17610
<span>Background : </span><span>Atopic dermatitis ( AD) is a chronic or chronically relapsing inflammatory skin disease with a prevalence ranging from 10% to 20% in children of developed countries. Skin-infiltrating leukocytes play a pivotal role in the initiation and amplification of atopic skin inflammation. The cytokines produced by T helper- 2 ( Th2) cells are crucial factors in the induction and maintenance of the disease . </span>
<span>Aim</span><span>: to study the percentage of expression and mean fluorescence intensity( MFI) of the activation marker CD38 and the chemokine receptor CXCR3 on peripheral blood CD3+ lymphocytes in children with atopic dermatitis . Also total serum IgE and absolute differential count were evaluated .This might be targets for therapy in disease . </span>
<span>Methods: </span><span>This study was conducted on thirty cases of AD children. Their age range was 3- 10 years. Also non atopic fifteen children age and sex matched with disease group were included as a control group. The percentage of expression of the CD38 , CXCR3 and MFI were analyzed by flow- cytometry on peripheral blood CD3+ T lymphocytes . Also total serum IgE levels was measured by immunonephelometry . The absolute eosinophil , absolute lymphocytes, absolute neutrophil count were evaluated . </span>
<span>Results: </span><span>The mean percentage of CD38 expression on CD3 + lymphocytes and MFI were 70.5% and 5.8 respectively in AD children compared with 17.8 % and 5.1 in non </span><span>–</span><span>atopic children healthy control ( p < 0.01 and p > 0.05 respectively ) . The mean percentage of CXCR3 expression on CD3+ T lymphocytes and MFI in AD children were 17.9 % and 2.9 respectively compared with 67.93 %and 3.3 in healthy controls (p < 0.01 and p > 0.05 respectively ). The mean of the total serum IgE in the patient group was 199.3 IU/ml compared with 62.27 IU/ml in non-atopic children (p < 0.01). </span>
<span>Conclusion </span><span>: </span><span>These results suggest that there is a relation between atopic conditions and an increase in peripheral blood T lymphocyte expressing CD38% and decrease expression of CXCR3%.The presence of high expression of CD38 in atopic patients seems to confirm the role of this molecule as an activation marker useful for evaluation of Th2 immune response . whereas CXCR3-expression on CD3+ lymphocytes decreased in AD than normal control as the chemokine receptor profile determine the migratory patterns of leukocytes . These results may suggest the dysbalance between Th1/ Th2 in AD patients . </span>
<span> </span>
CD 38,CXCR3,Flow- Cytometry,AD
https://ejhm.journals.ekb.eg/article_17610.html
https://ejhm.journals.ekb.eg/article_17610_703b87ce7f5c22172e4d0551a53b5432.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Electromagnetic Field Effect on Skeletal Muscles and Skin of Albino Rat Embryos Histological and Histochemical Studies
479
479
EN
Nora Ahmed Obaid
Alkaabi
Zoology Department , Girls College of Science, King Faisal University,Dammam,K.S.A.
10.21608/ejhm.2008.17611
<span>Aim of the work </span><span>:The present study aimed to investigate the possible effect of the electromagnetic field (EMF) on the skeletal muscles and skin of fetuses maternally exposed to EMF. </span><br /> <span>Material and Methods: </span><span>Pregnant Swiss albino rats were exposed to EMF(50Hz&2mT) 8hours day after day 10 days before pregnancy and 20 days, day after day through pregnancy. </span><br /> <span>Results: </span><span>Exposure of pregnant rats to the electromagnetic field (EMF)of 50 Hz and 2millitesla(2mT), showed many dystrophic changes in the skeletal muscles and skin of their fetuses .Highly thickened and disturbed epidermal layer was noted with decreased number of hair follicles. Some hemorrhagic areas were noted in the dermis. Highly disturbed skeletal muscle fibers were noted with many deleterious changes in the nuclei of their cells. Altered protein content, polysaccharides and collagen bundles were observed in the muscle fibers and skin of fetuses taken from exposed pregnant rats to EMF. </span><br /> <span>Conclusion</span><span>: It is clear that EMF has a deleterious effect on skin and skeletal muscles of fetuses maternally exposed to EMF. </span>
https://ejhm.journals.ekb.eg/article_17611.html
https://ejhm.journals.ekb.eg/article_17611_16d788392ac0c51412639aab06463e6d.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Relationship between polymorphisms in thrombophilic genes and preeclampsia In Egyptian population
503
509
EN
Nahla A.
Abdelaziz
Clinical Pathology Alazhar University
Fawzia A.
Alshishtawy
Clinical Pathology Alazhar University
Amal H .
Abu Saif
Obstet & Gynecol Alazhar University
10.21608/ejhm.2008.17612
<span>Background</span><span>: Hypertensive disorders are a major cause of maternal and fetal death especially in developing nations. </span><span>Preeclampsia has a familial component suggesting that one or more common alleles may act as susceptibility genes. Some families may have "private" predisposing mutations. Preeclampsia and its association with thrombophilia remain controversial, due to inconsistent results in different studies. </span>
<span>Aim</span><span>: The aim of this study was to evaluate the relationship between thrombophilic genes mutations and preeclampsia in pregnant women in our region. </span>
<span>Methods: </span><span>We compared 15 consecutive women with preeclampsia with 10 normal pregnant women. All women were tested for mutations of factor V lieden , Factor II (prothrombin gene ) , Factor XIII, B fibrinogen , plasminogen activator inhibitor -1 4G/5G (PAI-1 4G/5G ), </span><span>methylenetetrahydrofolate reductase </span><span>(MTHR) , </span><span>angiotensin </span><span>–</span><span>converting enzyme (</span><span>ACE) I/D , </span><span>apolipoproteins (</span><span>APO E and APO B ) gene</span><span>s. This study was based on reverse </span><span>– </span><span>hybridization technique using cardiovascular disease strip (CVD ) assay . </span>
<span>Results: </span><span>PAI-1 4G/5G polymorphism was highly significantly increased in patient group as compared to control group (60% versus 0% , p= 0.000) . No significant differences were noticed as regards other thrombophilic genes in control and patient groups.<br /> </span><span>Conclusion </span><span>We suggest that the pattern of PAI 4G/5G polymorphism might represent a useful marker of increased risk of preeclampsia in our region. Also our findings suggest that women with severe complications of pregnancy should be tested for markers of thrombophilia. </span>
<span> </span>
Thrombophilic genes,preeclampsia,Hybridization
https://ejhm.journals.ekb.eg/article_17612.html
https://ejhm.journals.ekb.eg/article_17612_2a532a8a9c56dd45590a48a5d6fb0f81.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
The Protective Effect of Soybean and Thyme on Iron Deficiency Anemia in Rats
510
520
EN
Nora M.
El-Sheikh
Department of Biochemistry and Nutrition, Women’s College, Ain Shams University, Cairo, Egypt
10.21608/ejhm.2008.17613
<span>Objective</span><span>: The present study is carried out to investigate the protective effect and antioxidant activity of soybean and thyme on iron deficiency anemia. </span>
<span>Material and Methods</span><span>: Thirty five male albino rats were divided into five groups (7rats each). The first group fed on basal diet, iron sufficient (35 mg Fe / kg), and served as control. Rats of other groups (second - fifth) were induced anemic by placing them on diet containing 3mg Fe / kg for 21 days, then divided to four dietary groups. The second group (anemic) stayed on basal diet with Fe-deficiency. The third group fed on basal diet with sufficient iron in the form of ferrous sulphate. The fourth group fed modified basal diet free from iron and supplemented with soybean. The fifth group fed basal diet free from iron and supplemented with thyme. All three iron sources provide 35 mg iron/kg diet. At the end of experiment (49 days), rats were anesthetized, whole blood was used for determination of hemoglobin (Hb), hematocrit (HCt) and reduced glutathione (GSH) levels. Serum was used for determination of iron and lipid profile as well as lipid peroxidation as malondialdehyde (MDA). The liver was used for determination of iron and copper concentrations. </span>
<span>Results: </span><span>The present results indicated that Fe-deficiency caused many adverse effects reflected the significant decrease of Hb, HCt, serum iron, liver iron, GSH and high density lipoprotein-cholesterol (HDL-C). Fe-deficiency also caused significant increase in total iron binding capacity (TIBC), liver copper, MDA, triacylglycerols (TG) and total cholesterol (TC). In contrary, administration of ferrous sulphate (FeSO</span><span>4</span><span>), soybean or thyme induced a significant increase of serum and liver iron profile. </span>
<span>Conclusion: </span><span>Soybean and thyme could able to provide iron to correct dietary iron- deficiency anemia and powerful </span>
iron deficiency anemia-soybean- thyme- lipid peroxidation
https://ejhm.journals.ekb.eg/article_17613.html
https://ejhm.journals.ekb.eg/article_17613_95524009619e2f0e60e0f498eb0de317.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Intrathecal Ketorolac Injection in Albino Rats; Pharmacological and Histological study
512
503
EN
Tarek A.
Atia
Histology Faculty of Medicine, Al-Azhar University, Cairo, Egypt
Mostafa I.
Shalaby
Anesthesiology & ICU Faculty of Medicine, Al-Azhar University, Cairo, Egypt
Nemat M.
Al-Baz
Pharmacology Departments; Faculty of Medicine, Al-Azhar University, Cairo, Egypt
10.21608/ejhm.2008.17614
<span>Introduction: </span><span>Ketorolac tromethamine is a potent injectable non-steroidal anti- </span>
<span>inflammatory drug (NSAID). Ketorolac provides successful analgesia after intrathecal or epidural injection. It is frequently used to manage post-operative pain, cancer pain, and arthritis either intrathecally, or intramuscular. However, its long term administration could induce renal toxicity and/or gastro-intestinal ulceration. </span>
<span>Aim of the study: </span><span>The aim of this study was to assess the analgesic potency of ketorolac after intrathecal injection. Also, we aimed to study the histological effect of ketorolac on the spinal cord and the duodenum after treatment in an animal model. </span>
<span>Methods: </span><span>40 adult male albino rats, weighing 250-350 gm, were used and divided into 4 </span><span>groups, 10 rats each. Group S (control) received 10μl normal saline intrathecally, group K50 received 50μg ketorolac intrathecally, group K50 + omeprazole (proton pump inhibitor) received 50μg ketorolac intrathecally plus </span><span>0.2 mg omeprazole orally, and finally, group K100 </span><span>received 100μg ketorolac intrathecally. All animals were treated for four successive days. </span>
<span>Result</span><span>: The rat tail flick latency was longer in K50, K50 + omeprazole, and K100 groups when compared to normal control (P = 0.002). Also, the hind-paw withdrawal latency was longer in treated groups when compared to those of the control group (P = 0.0001). Moreover, K50 group showed decreased phase II response by 61%, K50 + omeprazole group showed decreased phase II by 62%, while K100 group showed decreased it by 76%.<br /> Histological examination revealed no changes in the spinal cord of all treated animals. Also, examination of the duodenum showed normal duodenal mucosa in group K50 and those of group K50 + omeprazole. On the other hand, cellular infiltration as well as destruction of the mucous acini have been noticed in the duodenum of K100 group. </span>
<span>Conclusion: </span><span>Ketorolac could be a good alternative drug used intrathecally to manage pain. </span>
Ketorolac,analgesics,intrathecal,Rats
https://ejhm.journals.ekb.eg/article_17614.html
https://ejhm.journals.ekb.eg/article_17614_bf711163af4220c77052834b9899ef8c.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Using of Telomerase Enzyme in Urine as a Non invasive Marker for Cancer Bladder Detection
531
537
EN
Azza A
Hassan
Departments of Clinical Pathology Faculty of Medicine for Girls-Al Azhar University
Fawzia A .
El- Sheshtawey
National Institute of Urology & Nephrology-Cairo, Clinical Pathology
Seliem A.
Seliem
Urology Faculty of Medicine for Girls-Al Azhar University,
Mohammed A.
Abd El Salam
Pathology Cairo Faculty of Medicine
10.21608/ejhm.2008.17615
<span>Background</span><span>: Urinary bladder cancer is one of the major health problem all over the world. Cystoscopy remains the gold standard for identifying bladder cancer but it is invasive and expensive, therefore, a simple, non invasive test for detecting bladder cancer would be helpful. Several biomarkers for bladder cancer have been used, but no single marker has been accurate and conclusive. </span>
<span>Aim</span><span>: The current study aimed to measure telomerase enzyme in urine as a useful non invasive marker for detection of bladder cancer. </span>
<span>Methods </span><span>: Forty eight patients ( 39 males and 9 females) were included, They are complaining of urinary symptoms and undergo cystoscopy with biopsy of bladder lesions and histopathological examination. They were divided into groups: Group I: 16 patients ( 11 males and 5 females) have benign urologic conditions. Group II: 32 patients (28 males and 4 females) have proven bladder cancer patients underwent transurethral resection of bladder tumor or cystoscopy with biopsy of bladder lesions. Also, 15 apparently healthy volunteers with matched age and sex with patients were served as a control group. All subjects were submitted to laboratory estimation of the following in urine: urinary creatinine, urine cytology, telomerase enzyme in urine by telomerase PCR and complete urine examination. </span>
<span>Results : </span><span>The results of this study revealed that a highly significant increase in the frequency of cytolological positive cases for tumor cells in malignant group than each of benign group and healthy subjects, while no significant difference was detected between benign group and healthy subjects. The frequency of telomerase in urine was significantly higher in malignant group than each of benign group and healthy subjects, while no significant difference was detected between benign group and healthy subjects. The telomerase activity has sensitivity of 90.6% for diagnosis of cancer bladder with 93.7% for specificity and PPV was 96.6%, NPV was 83.3% and diagnostic accuracy of 91.6%. While, urine cytology gives a sensitivity of 68.8%, specificity of 87.5%, PPV of 91.6%, NPV 58.3% and diagnostic accuracy of 75%. When combined tests were used the sensitivity raised to 96,8%, and specificity reached to 100%, PPV was 96.6%, NPV was 94.1% and diagnostic accuracy increased to 97.9%. </span>
<span>Conclusion: </span><span>the urinary assay of telomerase could be used as non invasive test to identify the bladder cancer patients and distinguish them from normal subjects and patients with benign tumor of urinary bladder. The low cost of this test may help to be implicated as non invasive screening of bladder cancer. </span>
<span> </span>
Cancer Bladder,telomerase,PCR
https://ejhm.journals.ekb.eg/article_17615.html
https://ejhm.journals.ekb.eg/article_17615_17330c631783c51477a5c7003f680d69.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Effcts of the herbicide gallant and mercury on liver function of Tilapia zilIi
538
543
EN
Abdel-Salam Mohamed Ibraik
Ohaida
Zoology Department, Faculty of Science, 7th of October University, Misurata, Libya.
10.21608/ejhm.2008.17616
<span>Background</span><span>: Herbicides are highly toxic for both human and animal health. The increased application of herbicides in agriculture during the last decades has resulted in the contamination of both soil and water. Also do heavy metals, which represent one of the most important group of pollutants produced as a result of many industrial activities which can find their way easily to the normal aquatic environments, disturbing and damaging the existing organisms. </span>
<span>Material and Methods</span><span>: Fishes of the species </span><span>Tilapia zillii </span><span>were exposed to sublethal concentration of herbicide Gallant (haloxyfop-ethopxy ethanol ester) (3 mg/L), mercury as mercuric chloride (6 mg/L) and a combined dose of herbicide (1.5 mg/L) and mercury (3 mg/L) for 96 hrs., in aquaria under controlled laboratory conditions. A comparative physiological study was carried out to test the toxicological effects of these pollutants on glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) activities & hepatosomatic index of liver. </span>
<span>Results: </span><span>A progressive decrease in enzyme activity as well as total protein content of liver were observed, while hepatosomatic index showed a slight insignificant increase. </span>
<span> </span>
Gallant,mercury,glutamic pyruvic transaminase & glutamic oxaloacetic transaminase activities and hepatosomatic index of liver
https://ejhm.journals.ekb.eg/article_17616.html
https://ejhm.journals.ekb.eg/article_17616_de08bfc1bb569e85528577365f7c9b9c.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Evaluation of Hypoglycemic Activity of Opuntia dillenii Haw Fruit Juice in Streptozotocin-Induced Diabetic Rats
544
558
EN
Inas Z.A.
Abdallah
Nutrition and Food Science Dept., Faculty of Home Economics Helwan University
10.21608/ejhm.2008.17617
<span>Background: </span><span>Opuntia dillenii </span><span>Haw fruit is used in folk medicine as an antidiabetic agent. The aim of this study was to evaluate the possible curative role of </span><span>O. dillenii </span><span>fruit juice using the streptozotocin (STZ)-induced diabetic rats. The nutritive value of the edible portion of the fruit was also assessed. </span>
<span>Results: </span><span>The results showed that </span><span>O. dillenii </span><span>fruit is a rich source of fiber, carbohydrates, vitamins B</span><span>1</span><span>, B</span><span>2 </span><span>and C, in addition to the minerals, Fe, Zn, Cu, Cr, Mn, Ca, and Mg. Biological results showed that intraperitoneal injection with STZ caused highly significant reduction in body weight gain% , highly significant elevation in blood glucose concentration accompanied by significant reduction in liver glycogen cotent as compared with control group. Diabetic rats also revealed significant elevation in lipid peroxide (MDA) level, highly significant elevation in total cholesterol (TC), triacylglycerols (TAG), low-density lipoprotein cholesterol (LDL-C) and very low-density lipoprotein cholesterol (VLDL-C) concurrent with highly significant reduction in high-density lipoprotein cholesterol (HDL-C) as compared with control group. Oral administration of </span><span>O. dillenii </span><span>juice had no effect on normal rats. Meanwhile, oral administration of </span><span>O. dillenii </span><span>juice to diabetic rats induced siginficant improvement in body weight gain % and lipid profile, it reduced significantly blood glucose and MDA levels as compared with non treated diabetic group. Histopathological investigation of the pancreatic tissue of STZ-diabetic rats represented the presence of necrosis, edema and congested blood vessels in the islets of Langerhans cells. </span><span>O. dillenii </span><span>fruit juice treatment overcome the previous changes, the majority of the cells tend to be normal.The improvement in the cells of Langerhans islets may explain the </span><span>antidiabetic effect of the fruit juice under study. It also may improve the insulin receptors of β</span><span>- cells. </span>
<span>Conclusion: </span><span>It could be concluded that </span><span>O. dillenii </span><span>fruit juice had a potent hypoglycemic activity, this effect may be attributed to its antioxidant activity and its high content of chromium which was proved in this study. Therefore, it could be recommended that </span><span>O. dillenii </span><span>should be ingested as fresh fruit to diabetic and hypercholesterolemic patients beside the usual therapy. </span>
<span> </span>
Opuntia dillenii Haw fruit juice,nutritive value,Hypoglycemic activity,streptozotocin,diabetic rats
https://ejhm.journals.ekb.eg/article_17617.html
https://ejhm.journals.ekb.eg/article_17617_2a0b67d1009512f9f5d2cbd3c79cea6d.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Effects Of Orally Fed Diazinon On Some Biochemical Parameters Of Male Albino Rats
559
568
EN
Mohammed Salah Ab.Ab.
AL-Shinnawy
Biological and Geological Sciences Department , Faculty of Education , Ain Shams University , Cairo , Egypt .
10.21608/ejhm.2008.17618
<span>Background:</span><span>The current investigation was carried out to study the effect of orally administration of diazinon insecticide on some biochemical blood indices on male albino rats. </span>
<span>Material and methods: </span><span>The organophosphorus insecticide , diazinon was orally administrated at a dose<br /> (100mg/kg body weight=1/3LD50) , daily for 10 days (short </span><span>–</span><span>term) and 20 days (long </span><span>–</span><span>term) to adult male albino rats . </span>
<span>Results: </span><span>The results revealed the following: </span>
<span>1- Diazinon led to an increase in rat blood serum glucose& T3 (short and long </span><span>–</span><span>term); </span>
<span>AST,ALT, and T4 (long-term). </span>
<span>2- Diazinon reduced the serum content of total cholesterol and protein in short</span><span>–</span><span>term </span>
<span>treated rats. </span>
<span>3- Treatment of rats with diazinon did not alter activity of AST, ALT and T4 level at </span>
<span>short </span><span>–</span><span>term period .In addition ; ALP serum levels were not altered in both short and long </span><span>–</span><span>term treated rats . </span>
<span> </span>
Diazinon,Organophosphorus insecticide,Biochemical changes,serum
https://ejhm.journals.ekb.eg/article_17618.html
https://ejhm.journals.ekb.eg/article_17618_00f00b7531e7abbca3735f1b1139ed55.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Tumor necrosis factor- (TNF- ) in glutathione (GSH)-depleted rats. A possible link to impaired glucose metabolism
596
576
EN
Sohair A.
Moustafa
Department of Zoology, Faculty of Science, Suez Canal University, Ismailia, Egypt.
10.21608/ejhm.2008.17619
<span>Rats</span><span>: treated with the glutathione depleting agent, allyl alcohol (AlAl) (1 mmol/kg) was found to induce a marked increase in serum TNF- 45 minutes post treatment. This increase is suggested to play a critical role in the development of impaired glucose metabolism and glucose intolerance in AlAl-treated rats. Impaired glucose metabolism was evidenced by the significant increase in serum creatinine, urea and blood urea nitrogen accounting for accelerated glycolysis and breakdown of creatinine phosphate. These are the metabolic consequences of the activation of a back up system for the generation of ATP when the primary energy forming pathway is impaired. Meanwhile, the present data show a significant decrease in the serum levels of triglycerides and cholesterol in AlAl-treated rats that was accompanied with a concomitant increase in their liver levels indicating the development of fatty livers in these rats. Due to the strong link between TNF- and the GSH status and to the well established role of TNF- in causing insulin resistance, which is potentiated by fat accumulation in different tissues, it is concluded that the combination of TNF- overproduction, GSH depletion and lipid accumulation in the liver caused by AlAl treatment, cooperate making cells more sensitive to AlAl poisoning, therefore, imposing a potent negative impact on glucose metabolism. Added to the deleterious effects of TNF- , enhanced lipid peroxidation observed in AlAl- treated rats suggests possible alterations in the rates of glucose transport and metabolism which may further contribute to AlAl-induced impairment in glucose metabolism. </span>
<span>Conclusion</span><span>: Finally, the selective effect of TNF- in inhibiting insulin secretion give an additional support to its hypothesized role in initiating glucose intolerance in GSH-depleted rats. </span>
<span> </span>
TNF-,Rats,glutathione depletion,glucose metabolism impaired glucose metabolism
https://ejhm.journals.ekb.eg/article_17619.html
https://ejhm.journals.ekb.eg/article_17619_14c85084bdb93f33fcdc43e02b645da0.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
PCR detection of Toxoplasma gondii DNA versus serological diagnosis in women suffering from repeated abortion
577
586
EN
Abo
EL Naga
Zoology Department, Faculty of Science at Mansoura University, Egypt.
AM;
Shaltot
Genetics unit, children Hospital, Mansoura University, Egypt
AA; Rizk
EL- Baz
Genetics unit, children Hospital, Mansoura University, Egypt
Eman
fayad
Genetics unit, children Hospital, Mansoura University, Egypt.
10.21608/ejhm.2008.17620
<span>Background: </span><span>This study was conducted to test the utility of polymerase chain reaction (PCR) assay to detect recent infections with </span><span>Toxoplasma </span><span>in pregnant women. </span><span>T.gondii </span><span>DNA was detected by using B1 gene as a target for amplification which is highly specific for </span><span>T.gondii </span><span>and is well conserved among all of the tested strains. </span>
<span>Results: </span><span>This study revealed the following findings:(1) PCR was positive in 63 subjects, including 58 high risk cases (77.3%) and 5 of controls (12.5%). (2) 17 high risk cases (24.6%) had false positive IgM and 5 of controls (12.5%) had false negative result for IgM. (3) 17 high risk cases (32.7%) had false positive IgG and 5 of controls (12.5%) had false negative IgG. (4) No significant association between eating raw meat or contact with cats and positive ELISA for PCR but there is highly significant association between women with contact with soil and positive PCR. (5) No significant relation between residency and either ELISA or PCR. (6) Significant negative correlation between the age of the studied women and positivity of PCR</span><span>. </span>
<span>Conclusion</span><span>: this study highlights the need for a confirmatory test to detect primary acute toxoplasmosis in pregnant women. It demonstrates the possibility of defining and selecting the high-risk cases for mother-to-child transmission of infection by combining specific serology and PCR tests to formulate a specific approach </span>
<span> </span>
PCR,Toxoplasma gondii and Repeated abortion
https://ejhm.journals.ekb.eg/article_17620.html
https://ejhm.journals.ekb.eg/article_17620_6278682d67cebafe97833f7da5810026.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Anatomical, Histological And Histochemical Studies On Some Organs Of True Desert Rodents In The Egyptian Habitats
578
306
EN
Boshra A.
El- Salkh
Department of Zoology, Faculty of Science, Al-Azhar University for Girls
Hanaa A.
Khidr
Department of Zoology, Faculty of Science, Al-Azhar University for Girls
Zaki T.
Zaki
Department of Zoology, Faculty of Science, Al-Azhar University Boys
Mohammad I.
Basuony
Department of Zoology, Faculty of Science, Al-Azhar University Boys
10.21608/ejhm.2008.17622
<span>Aim of the work</span><span>: The present study aimed to compare between the anatomy, histology and histochemistry of two species belonging to two different families namely Muridae and Dipodidae. Muridae is the largest family of rodents in the world; it is represented in this work by fat sand rat </span><span>Psammomys obesus</span><span>. Four-toed jerboa </span><span>Allactaga tetradactyla </span><span>represents the other family, Dipodidae. </span><span>Psammomys obesus </span><span>lives in coastal presaharan region; the preferred habitat is saline marshes and wadis where halophytic plants were abundant. However, </span><span>Allactaga tetradactyla</span><span>, inhabites the salt marshes and clay desert areas of coastal plains. </span>
<span>Material and methods: </span><span>The investigated animals were collected from desert, weighed and transferred alive to the laboratory in separate cages then anaesthetized with ether, after which they were carefully dissected, organs are taken out and prepared for the histological and histochemical studies.<br /> The nucleocytoplasmic index of liver cells was calculated, kidney weighed and the relative thickness of cortex, outer and inner medulla was measured and total glomerular number was recorded. </span>
<span>Results</span><span>: The liver, as in mammalian species, is consists of five separate lobes. The mitotic index of </span><span>Psammomys obesus </span><span>is smaller than that found in </span><span>Allactaga tetradactyla</span><span>. This may be due to the increased activity of </span><span>Allactaga tetradactyla</span><span>.<br /> In the kidney, the cortex is classified into three regions namely superficial, midcortical and juxtamedullary zones. Both number and diameter of glomeruli, glomerular volume and relative glomerular blood volume is greater in </span><span>Psammomys obesus </span><span>than that in Allactaga tetradactyla. </span><span>Conclusion: </span><span>These observations indicate the ability of Psammomys obesus to produce highly concentrated urine than that of </span><span>Allactaga tetradactyla</span><span>. </span>
<span> </span>
Desert Rodents,histochemical,Liver,kidney
https://ejhm.journals.ekb.eg/article_17622.html
https://ejhm.journals.ekb.eg/article_17622_4a29f868070e891d4252876d62db8a65.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
The Toxicological effects of fenitrothion and vitamin E as antioxidant agent on the biochemical, cytogenetic and histopathological parameters of white rats
604
421
EN
Fouad
Abdel Reheim
Department of Biochemistry, Faculty of Agriculture, Cairo University
Awad Abbas
Ragab
Department of Biochemistry, Faculty of Agriculture, Cairo University
Fatma.M.
Hammam
Department of Mammalian Toxicology, Central Agricultural Pesticides Laboratory, Agricultural Research Centre.
Hossam El-Din
Hamdy
Department of Mammalian Toxicology, Central Agricultural Pesticides Laboratory, Agricultural Research Centre.
10.21608/ejhm.2008.17624
<span>Background: </span><span>The use of pesticides has been increased considerably nowadays compared to the past. The hazards of using such chemical compounds have been accentuated by the sharp rise of their use in agriculture, industry, by householders and governments. Exposure to organophosphorus insecticides (OPI) in agriculture is one of the occupational hazards. Fenitrothion is one of the most important OPI. The major object of the present study was to evaluated the toxicological (biochemical, mutagenic and histopathological) effects of tested insecticide "fenitrothion" alone or combined to vitamin E as an antioxidant agent to decrease their toxic effect. </span><br /> <span>Material and Method </span><span>male albino rats were tested orally for 30 days, three doses of fenitrothion were used in absence and presence of vitamin E (1/20, 1/40 and 1/80 LD50). </span><br /> <span>Results </span><span>the obtained data showed marked changes in biochemical parameter, highly inhibition of AchE activity; highly significant increase in the frequency of micronucleus (PCEM) in rat bone marrow cells at all doses of fenitrothion alone or combined to vitamin compared to control group. Also, the histopathological examination of liver and kidney tissues revealed high alternation in these tissues corresponding to biochemical changes. </span><br /> <span>Conclusion </span><span>From these results we concluded that fenitrothion exert biochemical, mutagenic and histopathological effects in white rats. In addition, vitamin E has mild role in alleviating these toxicological effects. </span><br /> <span> </span>
Organophosphorus,fenitrothion,antioxidant agent,micronucleus
https://ejhm.journals.ekb.eg/article_17624.html
https://ejhm.journals.ekb.eg/article_17624_cbb433904ffc2b2fcfd215ba3d7dc76a.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
33
1
2008
10
01
Nutrition Education intervention in dyslipidemic children and adolescent with insulin-dependent diabetes mellitus (IDDM)
639
649
EN
Asmaa m.
Abdallah
Clinical Nutrition Department, Nutrition Institute
Zainab
B
Clinical Nutrition Department, Nutrition Institute
Mohamed M. A.
Shahat
Department of Zoology, Faculty of Science, AL-Azhar University (Assiut)
10.21608/ejhm.2008.17625
<span>Background</span><span>: Diabetes mellitus is the most common endocrine metabolic disorder of childhood and adolescence with important consequences for physical and emotional development</span><span>. </span>
<span>Aim of the study: </span><span>This study was designed to detect the effect of diet therapy (through nutrition education program) on lipid profile and blood glucose level in diabetic children. </span>
<span>Subjects and Methods</span><span>: The study was carried on 45 diabetic children aged between 8-15 years old at diabetic nutrition clinic of nutrition institute in Cairo from 2003-2005. Children included in the study were divided into two groups: insulin dependent dyslipidemic group (IDDM) (diet control/ group) and insulin dependent non dyslipidemic (control group). All were subjected to full dietetic history by the 24 hour recall for 3 days, thorough clinical examination, they were evaluated for plasma lipids, lipoproteins, fasting blood glucose (FBG) and glycated hemoglobin (HbA</span><span>1c) </span><span>levels. The dyslipidemic were measured after three months for the previously measured parameters. The nutrition education process was performed and continued on weekly intervals for three months.<br /> </span><span>Results</span><span>: There was significant decrease in serum cholesterol and triglyceride levels in the study group after the program, and insignificant increase in serum HDL and decrease in serum LDL. Also, there was insignificant decrease in FBG but there was statistically significant decrease in HbA1 after the program. These changes occurred in parallel with increases in intakes of protein and total calories with adequate carbohydrate and sometimes a reduction in intakes of total fat</span><span>. Conclusion</span><span>: Nutrition therapy for children with IDDM is essential to improve measures of glycemic control and lipoprotein mediated risk for dyslipidemia. More innovative approaches to achieve lifestyle changes are required to meet current recommendations which are likely to produce greater beneficial changes than those observed in this study. </span>
<span> </span>
IDDM- Children- dyslipidemia- Diet control
https://ejhm.journals.ekb.eg/article_17625.html
https://ejhm.journals.ekb.eg/article_17625_0446172ac4e3934a41810c4a97485286.pdf