Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Evaluation the Efficacy of Immunotherapy in Treatment of Bronchial Asthma
295
305
EN
Seham
El-Mansi
Zoology Department, Faculty Of Science Damietta
Abd Allah
El-Bialy
Faculty Of Medicine Zagazig University
Mohsen
El-Shafey
Faculty Of Medicine Mansoura University
Shaimaa Mohamed Ebrahim
El-Sayed
Zoology Department, Faculty Of Science Damietta
10.21608/ejhm.2010.17379
<span>Abstract </span>
<span>The study was carried out on 50 allergic asthmatic children in rural district in sherbin,dakahlia and received their medication and immunotherapy from clinical immunology unit,zagazig university hospital.fifty patients were divided into 40 patients as active immunotherapy group and 10 patients as control group. </span>
<span>Immunotherapy was administered according to preseasonal schedule six month before pollen season.all patients have positive skin prick test and were evaluated clinically and immunologically pre and post immunotherapy (for six month), </span>
<span>Results </span>
<span>significant decrease in total serum IgE level P=0.018 (p<0.05)and significant increase in total serum IgG (P<0.001) after immunotherapy in all patients So immunotherapy is considered line of treatment for allergic patients. </span>
<span> </span>
Asthma,Chlidren,Total IgE,Total IgG
https://ejhm.journals.ekb.eg/article_17379.html
https://ejhm.journals.ekb.eg/article_17379_f9ac8920c47d8a24f0cb1d187fd62d51.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Identification and Characterization of Nuclear Matrix Protein(s) and assessment of its usefullness for early diagnosis of bladder cancer
306
313
EN
Abdelfattah M.
Attallah
R & D Department, Biotechnology Research Center, New Damietta City, Egypt
Seham E.
Mansy
Faculty of Science, Mansoura University, Damietta Branch, Egypt
Waheba A.
Zarouk
Molecular genetics and enzymology department, National Research Center,Cairo, Egypt
Ibrahim E.
Mohamed
Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt
10.21608/ejhm.2010.17380
<span>Background: </span><span>the highest incidence rates of bladder cancer are generally found in industrially developed countries, particularly North America and<br /> Western Europe, and areas associated with endemic schistosomiasis, including parts of Africa and the Middle East . The appropriate treatment of patients with bladder cancer mandates early detection and regular follow up for recurrences . Currently, cystoscopy is the standard method for diagnosing and monitoring bladder cancer recurrence, but it is an invasive and relatively costly technique, and may sometimes be inconclusive, particularly in cases of cystitis . </span>
<span>Patients and methods: </span><span>western blot and specific immunoglobulin-G antibody were used to identify the urinary NMP marker. Urine samples from 123 patients<br /> with bladder cancer and 50 controls were evaluated using the developed SDS-PAGE, Western blot and ELISA. </span>
<span>Results: </span><span>the NMP marker was identified in the urine of<br /> patients with bladder cancer at 52 kDa (NMP-<br /> 52) by SDS-PAGE, Western blot and ELISA. In addition, the NMP-52 tumor marker was not detected in the urine<br /> of patients . </span>
<span>Conclusion: </span><span>detecting the urinary NMP-52 marker using SDS-PAGE, Western blot and ELISA, would be helpful in the rapid diagnosis of bladder cancer. </span>
<span> </span>
bladder,Tumor,marker,NMP,urine,Diagnosis,SDS - PAGE,western blot,ELISA
https://ejhm.journals.ekb.eg/article_17380.html
https://ejhm.journals.ekb.eg/article_17380_d0ec002f6a21cd6a7ccc95a9bfd55e06.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Influence of Ionizing Radiation on Echis pyramidium Snake Venom: Biochemical and Immunological Aspects
314
334
EN
Adel G.
El-Missiry
Medical Research Center – faculty of medicine Ain Shams University
Esmat A.
Shaban
Department of Drug Radiation Research – National Center for Radiation Research and Technology – Atomic Energy Authority
Mohamed R.
Mohamed
Medical Research Center – faculty of medicine Ain Shams University.
Ahmed A.
Ahmed
Medical Research Center – faculty of medicine Ain Shams University.
Nadia M.
Abdallah
Department Of biochemistry – Faculty of Science Ain Shams University
Manal I.
Moustafa
Medical Research Center – faculty of medicine Ain Shams University
10.21608/ejhm.2010.17381
<span>The effect of a single LD</span><span>50 </span><span>dose of native </span><span>Echis pyramidum </span><span>venom (27.69μg/mouse) on the activities of certain serum enzymes levels: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), urea, creatinine, lactate dehydrogenase (LDH), creatine phosphokinase (CPK), creatine kinase isoenzyme (CK-MB) were studied. Samples from the serum were collected 4hr following LD50 venom dose intraperitonealy injected in male Swiss albino mice. The activities of these enzymes showed significant elevation compared to the non-envenomated group. In contrast, an equivalent dose of 1.5 kGy </span><span>γ irradiated </span>
<span>Echis pyramidum </span><span>venom (27.69</span><span></span><span>g/mouse) did not cause any significant increase compared to non-envenomated group.<br /> The effect of a dose that is equivalent to 1⁄2 LD</span><span>50 </span><span>(13.8 μg/50 μl) of native </span><span>Echis pyramidum </span><span>venom on plasma creatine phosphokinase (CPK) induced a significant increase of creatine phosphokinase (CPK) level compared to normal control (P<0.01). In contrast, an equivalent dose of 1.5 kGy </span><span>γ irradiated </span><span>Echis pyramidum </span><span>venom showed non significant difference in creatine phosphokinase activity when compared to the normal control. Light microscopic examinations of gastrocenemius muscles of mice injected with native </span><span>Echis pyramidum </span><span>venom (1⁄2 LD</span><span>50; </span><span>13.8μg/50μl) showed fragmentation, disorganization, loss of myofibrils in some of the muscle fibers, hemorrhage in-between the muscle fibers and mononuclear cellular infiltration. While light microscopic examinations of gastrocenemius muscles of mice injected with 1.5 kGy </span><span>γ irradiated </span><span>Echis pyramidum </span><span>venom (13.8μg/50μl; a dose identical to that used from native venom) showed that most muscle fibers were of normal appearance except for small area of fragmentation and disorganized myofibrils and oedema of the intercellular connective tissue. Double immunodiffusion test revealed a similar reactivity for native, 1 kGy, 1.5 kGy and 3 kGy </span><span>γ irradiated </span><span>Echis pyramidum </span><span>venoms against a commercial polyvalent Egyptian antivenin. The visible lines obtained in the immunodiffusion reactions were identical and joined smoothly at the corners, indicating that there was no change in their antigenic reactivity. These results demonstrate that the ability of the venom antigens to react with its corresponding antibodies was maintained in spite of being exposed to radiation doses of 1 kGy, 1.5 kGy and 3 kGy. </span>
<span>Both antivenins raised against native or 1.5 kGy </span><span>γ irradiated venoms </span><span>recognized </span><span>Echis pyramidum </span><span>venom when submitted to protein blotting, but the anti 1.5 kGy </span><span>γ irradiated venom </span><span>show a higher intensity bands than the antivenin raised against native </span><span>Echis pyramidum </span><span>venom, in spite of having less neutralizing activity (native neutralize 50 LD</span><span>50, </span><span>1.5 kGy </span><span>γ irradiated </span><span>neutralize 40 LD</span><span>50</span><span>), this indicates that antibodies were formed not only for toxic fraction but also for non toxic fraction. </span>
<span>Irradiation of the whole Echis Pyramidium Venom with 1.5KGy reduced its lethality 12.5 times though keeping its immunogenicity. The 1.5KGy dose was shown to be the best radiation dose to promote detoxification without significantly affecting its immunogenicity. Thus results of this </span><span>study confirm the conclusion that γ radia</span><span>tion is a suitable way to detoxify Echis Pyramidium Venom without affecting its immunogenicity provided that proper dose is used. </span>
Echis pyramidum,γ irradiated venom,aspartate aminotransferase,alanine aminotransferase,alkaline phosphatase,urea,creatinine,Lactate dehydrogenase,creatine phosphokinase,creatine kinase isoenzyme,Double immunodiffusion,protein blotting
https://ejhm.journals.ekb.eg/article_17381.html
https://ejhm.journals.ekb.eg/article_17381_46e7ba760974d72bc4a95ef2247b9034.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Haematological And Biochemical Changes Induced By Amaranth Impact On Male Albino Rats
335
349
EN
Shadia Ali
Radwan
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
Ahmed Riad
El-Sayed
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
Mohammed Salah
Al-Shinnawy
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
Omnia Nasr Abdel-Rahman
Mohamed
Department of Biological and Geological Sciences, Faculty of Education, Ain Shams University
10.21608/ejhm.2010.17382
<span>The present investigation was performed to study the effect of daily administration (interperitoneal injection) of synthetic coloring agent amaranth at dose 50 mg /kg b.w. /day for two periods (7 and 21 days), using male albino rats. The effects of amaranth on growth, haematological and some biochemical parameters were examined throughout the study. The results revealed that amaranth treatment cause a marked change in treated groups when compared with control. They showed a significant decrease in percentage of body weight change, RBCs count, Hb concentration and HCT value, while, they recorded a significant increase in WBCs, serum activity of AST and ALP, in addition to serum urea and creatinine levels in treated rats. But ALT and bilirubin showed insignificant changes throughout the experiment. </span>
<span> </span>
food additives,amaranth,haematological,biochemical,Parameters,albino rats
https://ejhm.journals.ekb.eg/article_17382.html
https://ejhm.journals.ekb.eg/article_17382_393805637b62e7cdd2e5954e54083fcf.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Low Dose Gamma Irradiation Modifies the Effect of L-Carnitine, Curcumin, Garlic Powder and Green tea Extract on Doxorubicin-Induced Nephropathy in Rats
350
364
EN
Rasha R.
Radwan
Department of drug radiation research, National Center for Radiation Research and
Technology, Atomic Energy Authority.
Esmat A.
Shaban
Department of drug radiation research, National Center for Radiation Research and
Technology, Atomic Energy Authority.
Hesham A.
Salem
Department of Pharmacology and Toxicology, Faculty of Pharmacy, CairoUni.
Sanaa A.
Kenawy
Department of Pharmacology and Toxicology, Faculty of Pharmacy, CairoUni.
10.21608/ejhm.2010.17384
<span>The possible protective potential of exposure to low dose of </span><span>γ </span><span>radiation in presence or absence of L-carnitine, curcumin, garlic powder or green tea extract was examined in the present study on doxorubicin (DOX)-induced experimental nephropathy in rats. Preliminary study was carried out to select the suitable dose of DOX to induce nephrotoxicity. In the current experiment 5 mg/kg, i.p. was selected as a single dose to induce nephrotoxicity during 15 days. The possible modulating effect of L-carnitine, curcumin, garlic powder or green tea extract on kidney function was examined. Animals were subdivided into three sets. Three groups of the 1</span><span>st </span><span>set </span><span>were exposed to γ radiation at a single dose level of 0.3 Gy then received DOX, 1, 3 or 7 </span><span>days postirradiation respectively. The groups of 2</span><span>nd </span><span>set daily received L-carnitine (40 mg/kg, i.p.), curcumin (50 mg/kg, i.p.), garlic powder (100 mg/kg, p.o.) and green tea extract (300 mg/kg, p.o.) daily for two weeks before induction of nephropathy. Groups of the 3</span><span>rd </span><span>set received the same doses of drugs then were injected with </span><span>DOX, 1, 3 or 7 days following γ irradiation </span><span>respectively. Two groups of animals, one of them received saline and served as normal and the other received DOX and served as nephropathic group were included in 1</span><span>st</span><span>, 2</span><span>nd </span><span>as well as 3</span><span>rd </span><span>set. Fifteen days following DOX administration, serum was collected and the animals were then sacrificed. Serum creatinine, urea and uric acid were evaluated. Data revealed that, a single DOX dose (5 mg/kg) induced marked acute nephrotoxicity manifested as significant increase in the activities of serum creatinine, urea as well as uric acid. Interestingly, pre-exposure </span><span>to γ </span><span>radiation at a dose level of 0.3 Gy, 1 or 3 days before DOX injection exhibited significant improvement in the above altered mentioned parameters. However, exposure to low dose radiation 7 days prior to DOX administration did not show a protective effect. Moreover, pretreatment with L-carnitine, curcumin, garlic powder or green tea extract in rats unexposed or exposed to </span><span>γ </span><span>radiation before DOX administration ameliorated, to a great extent, the effects induced by DOX. The present findings suggest that exposure to a single low dose of </span><span>γ </span><span>radiation (0.3 Gy) one day before DOX administration is a promising approach for maximizing the nephroprotective effects of L-carnitine, curcumin, garlic powder or green tea extract with minimal adverse effects of DOX. </span>
<span> </span>
Doxorubicin,0.3 Gy γ radiation,L-carnitine,Curcumin,garlic powder,Green tea extract,creatinine,urea and uric acid
https://ejhm.journals.ekb.eg/article_17384.html
https://ejhm.journals.ekb.eg/article_17384_4ba2fadb80d33a601da5f71ff96e8a3c.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Total IgE and Absolute Eosinophils Count as a Predictor of Allergic Diseases in Children
365
374
EN
Hamid
Habib
Consultant Pediatrician, Dean Faculty of Medicine – Rabigh - King Abdul Aziz University (KAU)
Moufag
Tayeb
Assistant Professor Faculty of Medicine - Rabigh - KAU.
Majdy
Qutub
Consultant Family Physician, King Abdul Aziz University Hospital.
Jamil
Samkari
Consultant Family Physician, King Abdul Aziz University Hospital.
Abdulrahman
Abu Dawood.
Demonstrator Faculty of Medicine - Rabigh – KAU.
10.21608/ejhm.2010.17385
<span>Objective</span><span>: To study the role of both serum total IgE levels and the absolute eosinophils count, total IgE alone, absolute eosinophils count alone as a marker of allergy in children, and to see their association with the host factors (age and sex) </span>
<span>Methods</span><span>: A retrospective study was conducted at King Abdul Aziz University Hospital </span><span>– </span><span>Jeddah (KAUH), during the year 2008. Three hundred children below the age of fifteen years meeting the inclusion criteria were enrolled for the study. Serum total IgE levels and absolute eosinophils count were done in all patients. Data was collected and tabulated. Chi-square was applied to test the association of the variables using SPSS and p-value of <0.05 was taken as statistically significant. </span>
<span>Results</span><span>: Out of 300 patients, 27(9%) had raised both serum total IgE and absolute eosinophils count, 146(48.67%) had raised serum total IgE alone, 40(13.3%) had raised absolute eosinophils count alone. Both IgE plus absolute eosinophilic count, total IgE alone and absolute eosinophilic count alone are not significantly related to the child sex with (P-values 0.759, 0.742, 0.699) respectively, however all are related significantly to the child age (P-values <0.004, <0.001, <0.012) respectively. All are not related significantly to systemic allergies except the significant relation between the absolute eosinophils count with atopic dermatitis (P- value <0.031) </span>
<span>Conclusion</span><span>: Serum total IgE level and absolute eosinophils count, total IgE alone and absolute eosinophils count alone are not a good predictor of allergy in children except that the absolute </span><span>eosinophils count can be considered as a strong predictor of atopic dermatitis in children. It’s </span><span>clear now, that as the child age increase the positivity of all the tests increase also. </span>
https://ejhm.journals.ekb.eg/article_17385.html
https://ejhm.journals.ekb.eg/article_17385_ae43f95dcade4544cfcda44ca0025e5d.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Optimization of fibrinase productivity from Actinomycetes
375
388
EN
Habib
S. A
ChemistryDepartment(BiochemistryDivision),FacultyofScience(Damietta),
Mansoura University, Egypt.
Abo doubara
M. I
BotanyDepartment,FacultyofScience(Damietta),MansouraUniversity,Egypt.
Abdel-Malak Camelia.
A
ChemistryDepartment(BiochemistryDivision),FacultyofScience(Damietta),
Mansoura University, Egypt.
Badawy Rasha.
M.
ChemistryDepartment(BiochemistryDivision),FacultyofScience(Damietta),
Mansoura University, Egypt.
10.21608/ejhm.2010.17386
<span>Background: </span><span>Fibrinolytic enzymes that dissolve blood clots and show promise for thrombosis therapy have been successfully identified from various sources. A wide range of microorganisms has been screened for their fibrinolytic properties. A fibrinolytic protease has been isolated from </span><span>Streptomyces violaceoruber </span><span>and </span><span>Streptomyces spiroverticillatus </span><span>culture filtrate. </span>
<span>Methods : </span><span>The purification procedure involved ammonium sulphate fractionation, dialysis, calcium phosphate gel purification and gel filtration on Sephadex G-100. By using native polyacrylamide gel electrophoresis (Native PAGE) to determine molecular weight of the enzyme. </span>
<span>Results : </span><span>The optimum temperature for the high production of fibrinase from </span><span>S. violaceoruber </span><span>was 30</span><span>o</span><span>C and from </span><span>S. spiroverticillatus </span><span>was 35</span><span>o</span><span>C and the optimum pH was 9.0. The best incubation period is 6 days. The incorporation of lactose as carbon source, yeast extract as nitrogen source and MnCl</span><span>2 </span><span>to culture media highly increased the production of fibrinase from the two species. The molecular weight was about 30 KDa. </span>
<span>Conclusion: </span><span>It exhibited fibrinolytic enzyme activity. </span><span>In vitro </span><span>studies revealed that fibrinase dissolves clots made by blood. </span>
Key words: Fibrinase,Fibrinolytic enzyme,fibrin clots
https://ejhm.journals.ekb.eg/article_17386.html
https://ejhm.journals.ekb.eg/article_17386_8f02ff6c555807a100416f9d9eeebc7f.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Expression of p-selectin (CD62p) on Platelets as activated marker in preeclampsia .
389
401
EN
Alshimaa A .
Abdel-Latif
Obstetrics and Gynecology Department Faculty of Medicine for Girls- Al Azhar University
Fawzia A.
EL.Sheshtawy
Clinical Pathology Faculty of Medicine for Girls- Al Azhar University
Amany A.
Bauomy
Clinical Pathology Faculty of Medicine for Girls- Al Azhar University
10.21608/ejhm.2010.17388
<span>Abstract </span>
<span>Aim</span><span>: The aim of this work was to study the platelet activation state by flow cytometer analysis of platelet expression of CD62p in patients with preeclampsia. </span>
<span>Methods</span><span>: This study was conducted on ten cases of mild preeclampsia (group I), their ages range was 22- 36 years and ten cases of severe preeclampsia(group II) their ages range was 20-35 years .Also ten normotensive pregnant women were included as a control group (group III) . The percentage of platelets expression of the CD61 , CD62p and MFI were analyzed by the flow- cytometr . </span>
<span>Results: </span><span>The mean percentage of CD62p expression on platelets and MFI were 67. 3% and 6.5 respectively in mild preeclampsia compared with 3.7 % and 1.5 in </span>
<span>normotensive pregnant as control ( p < 0.01 and p < 0.015 respectively ) . Also the mean percentage of CD62p expression on platelets and MFI were 73.3% and 2.1 respectively in severe preeclampsia, They showed significant increase when compared with normotensive pregnant as control ( p < 0.01 and p < 0.015 respectively ). There were a positive significant correlation between % of expression of CD 62p on platelets and SBP, DBP, protein in urine , and % CD61. While a negative significant correlation between % of expression of CD 62p on platelets and age, platelet count and CD62P MFI was found . </span>
<span>Conclusion : </span><span>: High levels of platelet glycoprotein CD62p expressions in patients with mild and severe preeclampsia, could be a compensatory mechanism for the preeclampsia induced thrombocytopenia . </span>
<span> </span>
<span>Background </span><span>: Pre-eclampsia is a medical condition in which hypertension arises in pregnancy (pregnancy-induced hypertension) in association with significant amounts of protein in the urine</span><span>. </span><span>Pre-eclampsia may develop from 20 weeks gestation (it is considered early onset before 32 weeks, which is associated with increased morbidity) </span><span>.</span><span>Platelets play an important role in the pathophysiologic mechanisms of preeclampsia. </span>
CD 61,CD62p,Flow- Cytometry,preeclampsia
https://ejhm.journals.ekb.eg/article_17388.html
https://ejhm.journals.ekb.eg/article_17388_7cee11630b4d896b30f83ae1cc1bfe7b.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Effects of sublethal doses of selected botanical molluscicides on oxygen consumption of the brown garden snail, Eobania vermiculata.
402
410
EN
Samir Mohamed H.
Beltagi
Department of Biological and Geological Sciences, Fac. of Education, Ain Shams University
Mohammed Salah. Ab. Ab.
Al-Shinnawy
Department of Biological and Geological Sciences, Fac. of Education, Ain Shams University
Nabawy Abdel-Rahman I.
Elkattan
Department of Biological and Geological Sciences, Fac. of Education, Ain Shams University
Hany Nady
Yousef
Department of Biological and Geological Sciences, Fac. of Education, Ain Shams University
10.21608/ejhm.2010.17389
<span>Laboratory bioassays were carried out for evaluating the efficacy of certain plant materials including Nicotine, Thymol, Menthol, Caffeine and Camphor as molluscicides against the brown garden snail, </span><span>Eobania vermiculata </span><span>using the topical application method. The obtained results proved that Nicotine and Thymol were the most promising from the molluscicidal point of view with LD</span><span>50 </span><span>204.02 and 551.20μg/snail for the t</span><span>wo materials, respectively. The effects of sublethal doses (LD</span><span>25 </span><span>and LD</span><span>50</span><span>) of the most potent materials, Thymol and Nicotine, on the oxygen consumption of </span><span>E. vermiculata </span><span>snails after 1, 7 and 15 days post treatment were evaluated. Results indicated that only snails treated with LD</span><span>50 </span><span>Thymol showed significant decrease in their oxygen uptake; while all the other treatments stimulated significant increase in the oxygen consumption along the three periods of estimation. This stimulation was most pronounced with the low sublethal doses of the both examined botanical molluscicides. The succession of time post exposure did not enable the treated snails to eliminate the adverse effects of the applied materials. </span>
<span>Depending on the results of the current investigation, we can conclude that the both examined botanical materials, Thymol and Nicotine, are effective in killing the agricultural pest </span><span>E. vermiculata </span><span>but further studies are needed to evaluate the efficacy of these materials as safe and economic molluscicides in the field. </span>
<span> </span>
molluscicides,Thymol,Nicotine,snails,Eobania vermiculata,Oxygen Consumption
https://ejhm.journals.ekb.eg/article_17389.html
https://ejhm.journals.ekb.eg/article_17389_89e3c30473fe1de8fe77de3c23e90316.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
MTHFR gene polymorphisms as risk factors for colorectal cancer among Egyptian patients.
424
434
EN
Rizk A.
El-baz
Genetics Unit, Children Hospital, Mansoura University, Egypt
Camelia A.
Abdel Malak
Biochemistry Department, Damitta Faculty Of Science, Mansoura University.
Saleh Ibrahim
El-Awady
Cancer Institute , Mansoura University, Egypt
Amira
Kazamel
Zology, Faculty of Science, Mansoura University, Egypt
Fatma A .
El tarpely
Biochemistry Department, Damitta Faculty Of Science, Mansoura University
10.21608/ejhm.2010.17392
<span>Objective: </span><span>Polymorphisms in methylenetetrahydrofolate reductase (MTHFR), such as MTHFR C677T and A1298C, are associated with several cancers. This study aimed to evaluate the effects of MTHFR polymorphisms on colorectal cancer risk in a population from damitta Egypt. </span><span>Methods</span><span>. This hospital-based case-control study was conducted during 2008-2010; 64 colon cancer cases and 90 controls were enrolled. Information was collected and blood samples were obtained for assay of MTHFR C677T and A1298C polymorphisms by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-restriction fragment length polymorphism (PCR-RFLP) techniques. Associations between variables of interest and colorectal cancer were assessed using conditional logistic regression. </span><span>Results: </span><span>Increased risk of colorectal cancer was associated with the MTHFR C677 TT genotype of C677T polymorphism (OR(adj) = 24.0; 95% CI: 1.34-429.1; P value for interaction = 0.001) . The 1298AC genotype and C allele was associated with a statistically significant lower risk among subjects (OR, 3.85; 95% CI, 1.78-8.33; P value for interaction=.0005 and OR,1.88 ;95% CI, 1.16-3.059 P value for interaction=0.01), respectively. MTHFR 1298 AA genotype and A allele was found to be associated with a significantly decreased risk for colorectal cancer (OR = 0.25, 95% CI 0.11- 0.52; P value for interaction= 0.0005 and OR = 0.52, 95% CI 0.32-0.85 P value for interaction= 0.01and ), respectively . There was no clear relation between colorectal adenomas and those with the 1298 CC genotype. The combined CC , AA [corrected] genotypes and the CT+AA [corrected] genotypes and the TT+ AC were associated with a statistically significant lower risk for developing colorectal cancer (P value for interaction= 0.03 , 0.02 , 0.001), respectively . </span>
<span>CONCLUSIONS: </span><span>The findings suggest an interaction between the MTHFR genotype and colorectal adenomas among Egyptian patients.<br /> Abbreviations: T2DM: Colorectal cancer</span><span>– </span><span>MTHFR: methylenetetrahydrofolate reductase </span><span>– </span><span>SNP: single nucleotide polymorphism- SSP-PCR: sequence specific primer- polymerase chain reaction. </span>
<span>Key words: MTHFR gene - Colorectal cancer</span><span>.</span>
<span> </span>
molluscicides,Thymol,Nicotine,snails,Eobania vermiculata,Oxygen Consumption
https://ejhm.journals.ekb.eg/article_17392.html
https://ejhm.journals.ekb.eg/article_17392_ad8380e0fcad792e352fcf7b9294df2d.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
Case Repert (Polymyositis and Interstitial Lung Disease associated with renal involvement)
435
441
EN
Jafar
Al-Momani
Pulmonary Division, Medicine Department, King Hussein Medical Center, Amman- Jordan.
Khalid
Al-Nadi
Pulmonary Division, Medicine Department, King Hussein Medical Center, Amman- Jordan.
Kher
Mbeidin
Pulmonary Division, Medicine Department, King Hussein Medical Center, Amman- Jordan.
Moh’d
Najada
Pulmonary Division, Medicine Department, King Hussein Medical Center, Amman- Jordan.
Abdullah
Al- Omari
Pulmonary Division, Medicine Department, King Hussein Medical Center, Amman- Jordan.
10.21608/ejhm.2010.17394
<span>Polymyositis with interstitial lung disease, renal involvement and positive Anti-Jo-1 antibodies in a 39-year-old male is reported. He presented with cough, dyspnoea and proximal muscle weakness. Chest imaging by X-ray and CT-scan showed wide spread reticulo-nodular densities. Urine analysis revealed RBCs Casts and albumiuria. Open lung biopsy revealed Non-specific interstitial pneumonia and muscle biopsy confirmed the diagnosis of polymyositis. Treating the patient with Prednisolone and Azathioprine showed dramatic improvement. </span>
https://ejhm.journals.ekb.eg/article_17394.html
https://ejhm.journals.ekb.eg/article_17394_dfe385205ba313413dd8810f17bc13ce.pdf
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
40
1
2010
07
01
MULTI-BIOMARKER RESPONSES TO BIOACCUMULATION of DICLOFOP-METHYL in FRESHWATER FISH (Oreochromis niloticus).
442
455
EN
Gamila, A. M.
Kotb
Mammalian and Aquatic Toxicology Dept.,
Manal E.A.
Elhalwagy
Mammalian and Aquatic Toxicology Dept.,
Sherif, H.
Abd Al-Rahman
esticides Residues and Environmental Pollution Dept.,
Central Agricultural Pesticide Laboratory, Agricultural Research Center, Giza, Egypt.
Ahmed, A. Gh.
Farag
Plant Protection Dept., Agriculture Faculty, Zagazig University, Egypt .
10.21608/ejhm.2010.17395
<span>The present study was undertaken to assess the environmental hazards presented to wildlife by new pesticides or new uses of established pesticides, biochemical changes, residual behavior and accumulation of diclofop-methyl (Iloxan 36 % EC) . A laboratory trials of diclofop-methyl were carried out on tilapia fish, Oreochromis niloticus as indicator species to . Acute toxicity of diclofop-methyl (Iloxan 36 % EC) was carried out and the median lethal concentration for 96 hours (96 h - LC50) value was 1.89 mg / l. In the present study, two groups of fish were exposed to two sub lethal concentrations selected on the basis of 1 / 10 (0.19 mg / l, low concentration) and 1 / 3 (0.63 mg / l, high concentration) of diclofop-methyl LC50 value for 28 days under the laboratory conditions compared to the control group. The treatments indicated that a gradual bioaccumulation of herbicide with time in whole fish was more pronounced than in muscle. Several criteria for estimating pesticide accumulation effect were studied on different biochemical parameters in plasma of fish after treatments. The results indicated that alanine aminotransferases (ALT), alkaline phosphatase (ALP) and acetylcholinesterase (AChE) activities as well as albumin and glucose levels increased significantly after treatment with both concentrations comparing to control group. However, significant increase in aspartate aminotransferases (AST) and total protein levels with was recorded low concentration (0.19 mg / l) only. On the other hand, high concentration (0.63mg / l) caused significant increase in urea levels. However, ceriatinine level was decreased in both exposed concentrations. Noticeable changes in antioxidants biomarkers were recorded , a reduction in SH-protein level accompanied with an elevation in lipid peroxidation biomarker (MDA) were recorded in plasma after treatment with two concentrations. The previous results were accompanied with noticeable alterations in plasma and muscle protein patterns in both treated groups. </span>
<span>Key words</span><span>: Diclofop-methyl, Herbicide, Bioaccumulation, Metabolic biomarkers, Fish, </span><span>Oreochromis niloticus</span><span>. </span>
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https://ejhm.journals.ekb.eg/article_17395.html
https://ejhm.journals.ekb.eg/article_17395_dad70da3c5b4c13a79586b9606bb2139.pdf