eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
463
474
10.21608/ejhm.2007.17693
17693
Original Article
Histopathological and Biochemical Toxic Effect of Amiodarone on Thyroid Gland in Albino Rat
Ola A. El Sayed
1
Safaa E. Gawish
2
Gihan. H. Aweida
3
Enas A. Auda
4
Anatomy Al-Azhar University, Faculty of medicine for girls
Forensic medicine & toxicology Al-Azhar University, Faculty of medicine for girls
biochemistry Al-Azhar University, Faculty of medicine for girls
pharmacology department Al-Azhar University, Faculty of medicine for girls
Backgrounds: Amiodarone AMD (Cordarone) was a benzofuran derivative, used in management of angina and refractory ventricular arrhythmia. Its effect on the thyroid gland structure and function was investigated in this study.
Material and Methods: Fifty adult male albino rats were used and divided into three groups. The first group was consisted of 10 rats which served as control, received distilled water orally (1ml). The second group was consisted of 20 rats used as therapeutic dose treated group, received 40 mg/Kg b. w. of amiodarone while the third group was consisted of 20 rats used as a toxic dose treated group which received 60 mg/Kg b. w. of amiodarone orally daily for three months. Body weight of animals was determined. Serum concentration of tri-iodothyonine (T3), thyroxine (T4), thyrotrophin (TSH), interleukin 6 (IL6), tumour marker P53 and tissue residue for amiodarone in plasma, fat, liver, lung, thyroid gland and heart was determined.
Results: Specimens from thyroid gland were taken and prepared for light and electron microscope examination. Highly significant decrease in body weight (P<0.001) were observed in both therapeutic and toxic doses treated groups in comparison to the control one. A very highly significant increase (P<0.001) of serum (T4 & T3) with Concomitant suppression of (TSH) (P<0.001). Serum levels of IL6 and P53 showed also a very highly significant increase (P<0.001). Amiodarone concentration in plasma, fat, liver, lung, thyroid gland and heart showed significant increase in therapeutic dose treated group and highly significant increase in toxic dose treated group.
Histopathological examination of thyroid gland of therapeutic dose treated group by light microscope showed marked evidence of thyotoxicosis in the form of microcystic follicular changes and peripheral scalloping, cellular degeneration with scanty cytoplasm and vesicular nuclei appeared. These changes became more severe in toxic dose treated group in the form of epithelial hyperplasia with atypical nuclear features. Thyroid tissue damage with haemorrhage and necrosis. Electron microscopic examination showed a remarkable cellular changes in the form of dilated rouph endoplasmic reticulum, inclusion lysosomes, dilated Golgi bodies, mitochondrial distension and nuclear degeneration. In both treated groups these changes were dose related.
https://ejhm.journals.ekb.eg/article_17693_ae0bc3fc3c5b70f895f282d2762c1a79.pdf
Amiodarone – Thyroid
gland
rat
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
475
485
10.21608/ejhm.2007.17694
17694
Original Article
Effect Of Dietary Supplementation With Tigernut Tubers On Streptozotocin-Induced Diabetic Rats
H. A. Hassan
1
Department of Zoology, Faculty of Science, Mansoura University, Mansoura, Egypt.
This work has been carried out to detect the effect of tigernut tubers as an antidiabetic plant on some biochemical parameters in streptozotocin (STZ)-induced diabetic rats. Diabetes mellitus was induced by a single intraperitoneal injection of 45 mg STZ /kg body weight. The present results indicated an increase in both serum glucose level and liver glucose-6- phosphatase (G6P) activity in STZ-diabetic rats. In addition, a significant decrease in serum insulin level and liver glycogen content was recorded in the same rats. Moreover, serum total lipids, total cholesterol, triglycerides and LDL-cholesterol levels revealed a significant increase, while a decrease in the level of HDL-cholesterol was observed in STZ-diabetic rats. The activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) showed marked increase in STZ-diabetic rats. On the other hand, tigernut- supplemented diet (25% w/w) for two or four weeks recorded a significant improvement in all the above biochemical parameters affected by STZ injection especially after four weeks of treatment. Therefore, it was concluded that tigernut tubers had appreciable hypoglycemic and hypolipidemic effects on STZ-diabetic rats.
https://ejhm.journals.ekb.eg/article_17694_6364cd7eda5cd4de9acb197899168ee1.pdf
Tigernut (Cyperus esculentus) – STZ-diabetic rats – lipid profiles
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
486
491
10.21608/ejhm.2007.17695
17695
Original Article
Genotoxicity of paracetamol on the germ cells of Drosophilla melanogaster
Saleha Y. M. alakilli
1
Department of Biology, Faculty of science, King Abdelaziz University, Saudi Arabia
Paracetamol is a common analgesic and antipyretic drug. The aim of the present study is to determine the potential genetic effects of Paracetamol in Drosophilla melanogaster using two methods: Sex Linked Recessive Lethals (SLRL) test and effect of Paracetamol on enzyme activity using spectrophotometric analysis. Three concentrations of drug were used (5, 10, and 20 mM). The results reveal significant differences in S.L.R.L, except spermatozoa stages showed insignificant increases when the data of the four broods were considered all together in three treatments. Meanwhile, Paracetamol showed a genotoxic effects in the three categories of the two generations of S.L.R.L, F1 heterozygous females, F2 bar eye females and F2 wild type males on the genetic back ground of Cholinesterase in all treatments.
https://ejhm.journals.ekb.eg/article_17695_76391aa4809e81fc88639f079ce5608c.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
511
521
10.21608/ejhm.2007.17696
17696
Original Article
Efficacy of oat bran (Avena sativa L.) in comparison with atorvastatin in treatment of hypercholesterolemia in albino rat liver.
Maisaa M. AL-Rawi
1
Biology Department, Girls College, Makkah, Saudi Arabia.
Introduction: The present study deals with the effect of oat bran (Avena sativa L.) in the treatment of hypercholesterolemia in comparison with a hypocholesterolemic drug, atorvastatin, on hypercholesterolemic liver in male albino rats.
Material and Methods: For this purpose four groups of rats (each containing 6 rats) were used. The first group was used as a control, the second was cholesterol-fed group with cholesterol (0.5% w/w) for 6 weeks. The third group was oats-fed hypercholesterolemic rats on oat supplemented diet (20% w/w) for 4 weeks and the forth group was atorvastatin-treated hypercholesterolemic rats orally at a dose of 0.18 mg/Kg body weight/day for 4 weeks.
Results: The biochemical results revealed a significant increase in serum LDL-C and a significant decrease in HDL-C level. In addition the activity of AST was increased in cholesterol-fed rats. Meanwhile, the treatment with oat soluble fiber or atorvastatin drug improved the above mentioned parameters. The histopathological examination of liver sections of cholesterol-fed group showed accumulation of lipid. Hepatocytes showed ballooning degeneration and manifested clear necrotic signs. Inflammatory cellular infiltration was found around the blood vessels, mild fibrosis near the portal blood vessels. However, liver impairment was reduced markedly in the liver of oat soluble fiber fed rats rather than atorvastatin drug treated rats.
Conclusion: The present study, however, confirms that the cereal grain oat may have potent beneficial health effects in reducing LDL cholesterol and should be included in the prudent diet of individuals with hyperlipidemia.
https://ejhm.journals.ekb.eg/article_17696_1adbfc2530422070d88cc58d0d4ea5c0.pdf
Liver
histopathology
oat bran
Atorvastatin
Hypercholesterolemia
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
522
536
10.21608/ejhm.2007.17697
17697
Original Article
Morphological, Biochemical and Ultrastructural Changes in the Pregnant Rat Placenta and the Liver of their Fetuses Treated with Folic Acid and / or Gamma Radiation
Fatma, L. Ramadan
1
Seham M. Abu Nour
2
National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA) Cairo, Egypt.
National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority (AEA) Cairo, Egypt.
Backgrounds: The efficacy of antioxidant supplementation and oxidative stress of gamma irradiation for and during pregnancy is poorly established. The present study aimed to detect the toxic effects of high dose of folic acid and / or gamma radiation on the placenta of pregnant rat and the liver of their fetuses. Material and Methods: Pregnant albino rats were divided into four groups. The first group served as a control, the second group received oral intake of folic acid (5 mg/kg) from the 5th to 20th day of gestation, the third group was irradiated with gamma radiation (3Gy, as fractionated doses (1Gy/ 3 times) on each 5th, 10th and 15th days of gestation, the fourth group was imanaged with combined treatment. The pregnant rats were sacrified after 20 days of pregnancy and samples were taken from the blood, placenta and the fetal liver for the morphological, biochemical and electron microscopic studies. Results: The present results showed a significant elevation in serum gamma glutamyltransferase (GT), lactate dehydrogenase (LDH) in placental tissue of pregnant rats associated with an increase of phosphorus content in liver of fetuses. Fetal malformations including: protrusion, anotia, short neck dactylomegaly, subcutaneous haemorrhage, paralysis in the fore limbs and congested blood vessels. The ultrastructural changes revealed sever damage in the placenta following folic acid administration and / or exposure to whole body gamma radiation. Also the fetal liver showed an appearent signs of damage under the combined treatment. The obtained changes were represented by: dilatation of the blood sinsoids, swollen mitochondria, fragmented rough endoplasmic reticulum and necrosis. Conclusion: It could be concluded that administration of folic acid and/or exposure to gamma radiation during pregnancy induced morphological, biochemical and ultrastructural changes in both placenta of the pregnant rats and liver of their fetuses.
https://ejhm.journals.ekb.eg/article_17697_0c7bda9bf21e2cb01afe7f3e86ac8194.pdf
Folic acid
Radiation
Pregnancy
placenta
Liver
fetus
-GT
LDH
phosphorus
Teratology
electron microscop
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
537
545
10.21608/ejhm.2007.17698
17698
Original Article
Effect of Trifolium alexandrinum extracts on the kidneys of diabetic rats: Biochemical and histological study.
Maisaa M. AL-Rawi AL-Rawi
1
Biology Department, Girls College, Makkah, Saudi Arabia.
Diabetic nephropathy is one of the most common and most devastating complications of diabetes. During the course of diabetic retinopathy, the glomeruli are slowly destroyed, usually with no detectable signs until the later stages of the disease, when the kidneys filtration process can no longer function. Throughout this disease, blood flow through the kidneys increases "hyperfiltration", and the kidneys become enlarged. Damage to the glomeruli becomes evident, as well as a condition known "microalbuminuria", in which a blood protein, albumin, leaks into the urine. This loss of proteins and other nutrients in the blood progresses, as does the damage to the glomeruli. The kidneys progressively lose their ability to filter waste until the final stage, kidney failure, occurs. On the other hand, some species of the plants were reported having remarkable medical importance. Trifolium alexandrinum is a medicinal herb; it has been shown to improve the flow of blood through arteries and veins in diabetic patients. Therefore, the present study aims to determine the possible improvement effects of different extracts of Trifolium alexandrinum (CF) on some biochemical and histopathological changes in the kidneys of streptozotocin-induced diabetic rats. Sixty adult male albino rats (210 ± 5 g) were injected (IP) with streptozotocin (STZ) (50 mg/kg b.wt) for induction of diabetes. Animals were classified into five groups. Daily intake of water, hexane and ethanolic extracts of T. alexandrinum in drinking water for 4 weeks after diabetes induction were used. Urine glucose was detected every week. Serum total protein, albumen, urea, uric acid and creatinine levels were tested. Data were expressed statistical to elucidate the differences between treated and control groups. The kidney paraffin sections of control and treated groups were stained with haematoxylin and eosin for histological studies. A single dose of STZ induced diabetes produced remarkable effects on both structure and function of the rat kidneys. The kidney exhibited damage of some renal corpuscles revealed glomerular mesangial cells hypertrophy, dilatation of renal tubules together with necrosis of their lining cells. Serum total protein, albumen, urea, uric acid and creatinine were significantly increased. Daily urine volume and consequently renal excretion of albumen, urea, uric acid and creatinine were significantly increased. However, remarkable improvement of the renal structure and function of diabetic rats was observed after treating with Trifolium alexandrinum extracts especially the group treated with water extract.
Conclusion: Extracts of Trifolium alexandrinum improved histological and biochemical alterations of the kidneys noticed in STZ-diabetic rats. These effects may be due to the presence of a high content of flavonoids which acts synergistically as antioxidants.
https://ejhm.journals.ekb.eg/article_17698_9fea9584faa89e61f0ca254a08722dc6.pdf
Trifolium alexandrinum – kidney function – STZ-diabetic rats
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
546
556
10.21608/ejhm.2007.17699
17699
Original Article
The Protective Effect of Curcumin Against Gentamicin-Induced Renal Dysfunction and Oxidative Stress in Male Albino Rats
Bushra H. El-Zawahry
1
Effat M. Abu El Kheir
2
Physiology Department Faculty of Medicine Al -Azhar University-Cairo
Biochemistry Department Faculty of Medicine Al -Azhar University-Cairo
Background: Generation of free radicals in the renal cortex plays an important role in the pathogenesis of gentamicin-induced nephrotoxicity. Curcumin, the yellow curry pigment isolated from turmeric, has been confirmed to have a strong antioxidant and free radical scavenging actions. In the present study, we investigated the possible protective effect of curcumin against gentamicin-induced nephropathy in male albino rats.
Methods: Thirty two male albino rats were divided into 4 equal groups (Gr.): (Gr.I) control, injected i.p with 1cc isotonic saline solution/day for 8 wks; (Gr.II) received 200 mg/kg/day curcumin (Cur. ) orally, suspended in normal saline, for 8 wks ; (Gr.III) injected I.p by 100 mg/kg/day gentamicin (Gen.) for 8 days followed by 1cc saline I.p thereafter; (Gr.IV) (Cur./Gen.) received 200 mg/kg/day Cur. for one week before starting Gen injection (100 mg/kg/day) for 8 days during which Cur. was received concurrently with Gen then Cur administration was continued thereafter throughout the rest of the study (6 wks). Body weight was recorded weekly. Renal function was evaluated by measuring the 24 h. urine output, the concentrations of plasma creatinine, blood urea nitrogen (BUN) and creatinine clearance. Also, kidney weight and the parameters of oxidative stress: reduced glutathione (GSH), thiobarbituric acid reacting substance (TBARS) and the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPX) were measured in kidney tissue.
Results: The Kidney weight, plasma creatinine, BUN and 24 h urine output were significantly increased while the body weight and creatinine clearance were significantly decreased (P < 0.0005), in rats treated with Gen. as compared to control. While Cur. could significantly normalize the previous parameters. In addition Gen. caused oxidative stress in kidney as seen by significant increase in TBARS level, and significant decrease of catalase, GSH, SOD and GPX activities (P < 0.0005), However, Cur. could normalize all the above parameters as compared to control.
Conclusion: Our data indicate that Cur. could suppress renal toxicity by blocking oxidative injury in the kidney and restore the antioxidant enzymatic profile. The renoprotective effect of Cur. is also evident by a remarkable improvement of renal function in Gen. injected rats. So Cur. can be used as a potent protective agent against renal oxidative damage mediated by Gen.
https://ejhm.journals.ekb.eg/article_17699_83cd61a4034bcd5bba8ca622c2c623b6.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
557
565
10.21608/ejhm.2007.17700
17700
Original Article
Role of Secretory Excretory Products of Schistosoma Mansoni Eggs in Modulating Hepatic Morbidity
Ibrahim Rabia
1
Zeinab Fahmy
2
Hoda Sabry
3
Eman El-Ahwany
4
Parasitology Department Theoder Bilharz Research Insitute, Giza ,Egypt
Parasitology Department Theoder Bilharz Research Insitute, Giza ,Egypt
Parasitology Departments Theoder Bilharz Research Insitute, Giza ,Egypt
Immunology Departments Theoder Bilharz Research Insitute, Giza ,Egypt
In the present study the possible anti-morbidity effect of secretory excretory products (SEP) of Schistoma mansoni eggs (given to mice before infection) was investigated. Multiple small doses of SEP were injected intra-peritoneally into albino mice (100 μg of purified SEP followed 2 weeks later by two booster doses of 50 μg each at weekly intervals). Data revealed reduction in CD4+ cells and increase in CD8+ cells of hepatic granuloma in SEP-immunized infected group, resulting in significant decrease in CD4+/CD8+ ratio, in comparison to infected control group. The serum cytokine level of both TNF--alpha and IFN-gamma were also significantly decreased. Histopathological examination of liver revealed remarkable increase in degenerated ova within hepatic granuloma which decreased in diameter (12%). Significant reduction in worm burden (46%) and tissue egg loads (42.8% and 50% for hepatic and intestinal ova respectively) were observed. Mean while decreased percent of immature stages with increase in percent of dead ova in Oogram pattern was recorded. This work may help in decrease the severity of hepatic morbidity.
https://ejhm.journals.ekb.eg/article_17700_c2b0a1b30d708bfe4ae6c5893cd24541.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
566
577
10.21608/ejhm.2007.17701
17701
Original Article
Immunohistochemical Expression of P53 and Cyclin D1 Proteins in Follicular Thyroid Tumors: Evaluation of Their Significance
Ahmed H. Abdel-Rahman El-Rashidy
1
Samir Abdel Salam A. Ibraheim
2
Amer Yehia Mohamed
3
Mohammed Hassan Fahmy
4
Shehata AM
5
Departments of Pathology Al-Azhar(Assiut), Cairo & Al- Menya Universities
General Surgery Al-Azhar(Assiut), Cairo & Al- Menya Universities
General Surgery Al-Azhar(Assiut), Cairo & Al- Menya Universities
General Surgery Al-Azhar(Assiut), Cairo & Al- Menya Universities
General Surgery Al-Azhar(Assiut), Cairo & Al- Menya Universities
The sequential mutational events which may underlie the tumorigenesis of thyroid neoplasia are gradually becoming apparent. To clarify the role of P53 and cyclin D1 in the oncogenesis and tumor progression of thyroid neoplasms, we examined the immunoreactivity of these proteins in one hundred and thirty (130) thyroid tumors originating from the follicular epithelium using immunohistochemistry. The tumors were divided into two groups group I: included thirty five (35) follicular adenomas and group II: included ninety five (95) follicular carcinomas; 65 of which were well differentiated (WDC) while the remaining (30) were poorly differentiated (PDC). P53 positivity was more frequent in group II (20/95; 21.1%) than in group I (6/35; 17.1%) and in PDC (11/30; 26.6%) than WDC (9/65; 13.8%). On the other hand, cyclin D1 positivity was frequent in WDC (21/65; 32.3%) and rarely seen in adenoma group (2/35; 5.7%). Co –positivity for P53 and cyclin D1 proteins was more observed in PDC (5/30; 16.7%) than in WDC (3/65; 4.6%).
The study suggested that cyclin DI may be involved in the thyroid oncogenesis and concluded that both proteins may be incriminated in the progression of follicular thyroid neoplasms. Moreover, we found that age at the time of diagnosis, the histologic differentiation, necrosis in primary tumor, extrathyroidal invasion and the presence of distant metastases are important prognostic and risk factors. In addition, our study revealed that the detection of P53, but not cyclin D1, in primary follicular carcinomas is a significant independent prognostic indicator which, together with the above mentioned important prognostic factors, may be of value in the theraputic planning of these tumors.
https://ejhm.journals.ekb.eg/article_17701_73f87b93738c3cf0ef2ed7dbb6d2b763.pdf
Thyroid gland – Follicular tumors – Immunohistochemistry- P53 – Cyclin D1- prognosis
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
578
590
10.21608/ejhm.2007.17702
17702
Original Article
Cytokines, Lipid Peroxide and Nitric Oxide in Egyptian Hepatocellular Carcinoma on Top of HCV and HBV Infection
Background and Aims: Many studies have shown the relative roles of hepatitis B and C viruses in hepato-carcinogenesis. The aim of this study is to define the independent and interactive roles of some cytokines namely, TNF , IL-6, IL-1 together with NO and TEARS in the genesis of HCC following the infection with such viruses.
Patients and methods: Blood samples were taken from 58 patients with hepatocellular carcinoma and were divided into four groups: a) 28 patients with HCV, b) 10 patients with HBV, c) 11 patients with B+C, d) 9 patients without viral infection. In addition, 20 healthy subjects served as control group for each, TNF , IL-6, and IL-1 were measured using
ELISA technique, in addition to NO and TBARs using chemical methods. Results: Patients with coinfection B-C viral infection showed the highest levels in
studied parameters. Patients with HCV and HBV separately showed more or less similar results. However, patients without viral infection showed the least higher levels comparing to the control group.
Conclusion: Cytokines in addition to NO and TEARS have a definite role in hepatic carcinogenesis. Coinfection with the two viruses carries a synergistic risk factor of hepatocellular carcinoma development. Depending on the results of the studied parameters HCV did not show predominancy on HBV. Further studies are needed to clarify the exact mechanism of carcinogenesis especially in HCV patients.
https://ejhm.journals.ekb.eg/article_17702_4666956f3671e32fe699dd5cad9fe15a.pdf
HCC
HCV
HBV
IL-1
IL-16 TNF
NO. TBARs
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
591
603
10.21608/ejhm.2007.17703
17703
Original Article
Effects Of Aging On Pancreatic Islet Cell Function : An Experimental Immunohistochemical And Ultrastructural Study
Ahmed H. Abdel-Rahman
1
Hassan Abdel-Hady Hassan
2
Mohamed A. Kasem
3
Hosam El-Din H. Osman
4
Hanan S. Abdel-Hamid
5
Mohamed El- Basil
6
Mohammed A. Mashahit
7
Departments of Pathology Al-Azhar Faculty of Medicine (Assuit& Cairo)
Departments of Pathology Al-Azhar Faculty of Medicine (Assuit& Cairo)
Histology Al-Azhar Faculty of Medicine (Assuit& Cairo)
Anatomy Al-Azhar Faculty of Medicine (Assuit& Cairo)
Departments of Pathology Kasr El-Aini Faculty of Medicine (Cairo)
Internal Medicine Kasr El-Aini Faculty of Medicine (Cairo)
Department of Internal Medicine Al-Fayoum University.
A strong relationship between aging and diabetes mellitus has been clinically suggested, however, none of the previous published data had clearly focused on the age-related cytomorphological changes in the pancreas which are the goal of this study.Three groups of male apparently healthy rabbits have been used, ten animals each; classified as group-1 (3- 5months old); group-2 (9-12 months old) and group-3 (24-36 months old). After sacrification, sections from the pancreas were stained by Haematoxylin and Eosin (H&E), Gomori trichromic stain & ultrastructurally to detect aging histologic changes as well as immunohistochemically to identify insulin and glucagon secreting cells using their appropriate monoclonal antibodies .
A progressive histological distortion with fibrosis and fatty changes were directly proportional to age, being mild in group-2 and severe in group-3. Morphometric studies by computerized image analysis showed that the mean number of islets was significantly higher in group2 (8.98±1.51), lowest in group-1 (5.08±1.48) and intermediate in group-3 (6.37±1.37). The mean diameter and square area of islets were significantly higher in group-2 compared to other groups (P< 0.05). The mean number of cells per islet & their secretary granules were significantly (P <0.05) higher in group-2, intermediate in group-1 and lowest in group-3.In contrast, the mean number of cells per islet and their secretory granules were insignificantly (P< 0.05) higher in group -2, intermediate in group-3 and lowest in group-1.Also, the / ratio ( cells/ cells) was greatest in group-2 (3.059:1), intermediate in group-1 (3.37:1), and lowest in group-3 (2.479:1).
The increased number of cells may be due to a compensatory process to correct the hormonal feedback mechanism of insulin .The results of this work suggest that cells are generally more vulnerable to aging, an observation which might be correlated clinically with higher incidence of diabetes in older ages.
https://ejhm.journals.ekb.eg/article_17703_2b262549d37c13d996288c6ff10aec36.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
604
615
10.21608/ejhm.2007.17704
17704
Original Article
Nutritional Status of Mentally Disabled Children in Egypt
Asmaa M AbdAllah
1
Shawkia S. A. El-Sherbeny
2
Sahar Khairy
3
Clinical Nutrition Department, Nutrition Institute.
Nutritional Biochemistry Department, Nutrition Institute
Nutritional Requirement and Growth Department, Nutrition Institute
Introduction: Mental disability can interfere with education and scholastic achievement. It can lead to school dropout and minimize opportunities to participate in the labor force. Moreover, disabilities affect the overall health and psychological state of the individuals.
Objective: The aim of the present study was to assess the nutritional status of mentally disabled children in Egypt on the basis of anthropometric indicators and laboratory data.
Subjects and Methods: Across sectional study was conducted on 639 mentally disable children aged 6-<14 years (422males and 217females), from urban areas. They were classified into two age groups (6-<11& 11-<14 years). The following variables were determined using Z score indicator of weight for age, height for age and weight for height. Also, body mass index, mid arm circumference and triceps skin fold thickness percentiles were determined. Laboratory investigation including blood hemoglobin concentration and plasma levels of vitamin A and α- tocopherol as well as plasma zinc, copper and magnesium concentration were estimated.
Results: Prevalence of underweight was 14.1% by Wt/age Z score. Stunting was estimated among 33.5% with significantly higher prevalence in older children. Muscle &fat depletion was detected in 30.2%, with significantly higher prevalence among males than females. Depleted fat store was detected in 14.7% of children. Wasting was documented in 14.1% of children by BMI percentile, and was significantly higher among males than females. No significant difference was detected between male and female handicapped children regarding mean blood hemoglobin values as well as mean plasma levels of the measured vitamins and minerals, except for mean plasma zinc level. The overall prevalence of iron deficiency anemia was higher in males (41.5%) than females (37.1%). Deficiency of α- tocopherol was more prevalent than that of vitamin A. The overall prevalence of deficiency of zinc, magnesium and copper was 1.8%, 40%, and 25.3% respectively. The decline in anthropometric measurements was associated with the decline in socioeconomic status.
Conclusion: Malnutrition as revealed by anthropometric variables and micronutrient deficiency occurs with a high prevalence among mentally disabled children and almost increased with age and low socioeconomic level.
https://ejhm.journals.ekb.eg/article_17704_ad91786c20c299a172388149638c5b7a.pdf
Mental handicap- Children- Anthropometric measurements- Social status- Blood hemoglobin- Plasma- Vitamin A- α-tocopherol- Zinc-Copper-Magnesium
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
616
630
10.21608/ejhm.2007.17705
17705
Original Article
Prevalence, Risk Factors and Impacts of Schistosomal and Intestinal Parasitic Infections Among Rural School Children in Sohag Governorate
H. M. El-Masry
1
Y. A. Ahmed
2
A. A. Hassan
3
S. Zaky
4
E. S. Abd-Allah
5
E. A. El-Moselhy
6
M. A. Abdel-Rahem
7
Departments of Pediatrics; Tropical Medicine Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Departments of Pediatrics; Tropical Medicine Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Departments of Pediatrics; Tropical Medicine Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Departments of Pediatrics; Tropical Medicine Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Departments of Pediatrics Community Health Nursing Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Departments of Pediatrics Community Medicine Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Departments of Pediatrics,Parasitology,Faculty of Medicine and Nursing, Al-Azhar and Zagazig University
Parasitic diseases represent a major cause of morbidity and mortality in childhood in most parts of the world. Hygiene and play habits make children especially vulnerable to schistosomal and parasitic infections. The aim of this study is to define the prevalence of different types of parasitic infections, to define their risk factors and to determine their impacts on health and scholastic absenteeism and achievement of rural school students in Sohag Governorate, Egypt. A cross-section, analytical study design was chosen to perform this research on 960 rural school students. All the students were interviewed and examined clinically and laboratory. The study showed that 38.5% of the students were infected by parasites. Entaemoeba histolytica, Enterobius vermicularis and Giardia lamblia had the highest percentages, 20.4%, 16.6% and 15.2%, respectively. Male sex, last birth order, poor personal hygiene, low socioeconomic level, ≥3 infected siblings, previous parasitic infections and no early consultation for therapy were important risk factors (ORs=1.41, 2.32, 2.63, 2.86, 4.17, 9.80 and 10.83, respectively). Also, 29.2% and 31.6% of infected students were below the 5th percentiles as regard weight-for-age and height-for-age, respectively. Anemia was present among 52.4% of infected students. Further, 3.2% of them had hepatomegaly. Also, 37.8% and 41.1% of infected students had 0-3 and 4-6 days/month absent, respectively, while, 34.3% of infected students had a scholastic achievement <50.0%. Improving personal and environmental hygiene and regular screening, treatment and health education for students as regard parasitic infections in Egypt is recommended.
https://ejhm.journals.ekb.eg/article_17705_13aad2c0e981d83ecd39388266266c8d.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
631
646
10.21608/ejhm.2007.17706
17706
Original Article
Possible protection of Vitamin E and Alpha-lipoic acid against early changes in alloxan diabetic rats.
Sohair A. Moustafa
1
Nahla S. El-Shenawy
2
Amro M. Elgheznawy
3
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Diabetes mellitus is a chronic syndrome affecting carbohydrate, fat, protein and nucleic acid metabolism. The current study was undertaken to elucidate the possible role of vitamin E and alpha lipoic acid in combination as an antioxidant and a biological membrane stabilizer in the protection against early complication of diabetes. Administration of alloxan (125 mg/kg wt, i.p.) to rats resulted in hyperglycemia, hyperinsulinemia, hypercholesterolemia, hypertriglyceridemia, hyperlipidemia, increase in plasma levels of urea, blood urea nitrogen (BUN), creatinine, uric acid as well as pancreatic thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) content of both liver and retina. These changes were accompanied with significant decrease in plasma total protein, tumor necrosis factor α (TNFα), hepatic catalase activity (CAT), and TBARS level of both liver and retina as compared to control group. However, plasma levels of calcium ions (Ca+2) and nitric oxide (NO) as well as pancreatic GSH content were not changed. On the other hand, the daily treatment of the diabetic rats with antioxidant mixture attained a reduction in plasma levels of glucose, cholesterol, triglycerides, total lipids, urea, BUN, creatinine, uric acid, TNFα, pancreatic TBARS level as well as GSH content of both liver and retina. In contrast, the daily treatment caused an increase in plasma levels of insulin, total proteins, hepatic CAT activity and pancreatic GSH content as compared to diabetic rats. However, plasma levels of Ca+2 and NO as well as TBARS content of both liver and retina were not affected. In conclusion, it is obvious from the present study results that early stage (two weeks) of diabetes induce deteriorate changes in carbohydrate, lipid, protein and nucleic acid metabolism accompanied with increasing of oxidative stress in pancreas as compared to both of liver and retina. Moreover, the data of present study indicated the effective role of vitamin E and alpha lipoic acid combination in combating the oxidative stress via its improvement to metabolism of carbohydrates, lipids, proteins and nucleic acids in addition to its free radical-scavenging and antioxidant properties.
https://ejhm.journals.ekb.eg/article_17706_3a69d60d7d3835bdfe831eecc29c14a4.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
647
663
10.21608/ejhm.2007.17707
17707
Original Article
Preparation of 99mTc-Carnosine and 99mTcO-(V)-DMSA Complexes, Biological Distribution, and Estimation of Their Gene Anti- Polymorphisms Induced by -Irradiation
E.A. EL-Ghany
1
F. Marzouk
2
Samy A. Abd El-Azim
3
M.A. Barakat
4
M.H. Awwad
5
Labeled Compound Department, Hot Lab. Center, Atomic Energy Authority, Egypt
Labeled Compound Department, Hot Lab. Center, Atomic Energy Authority, Egyp
Faculty of pharmacy, Cairo university
Faculty of pharmacy, Cairo university
Faculty of Science, Benha university
Background: Two chelating agents (Carnosine and DMSA) were used to study their labeling conditions with technetium-99m followed by biological distribution investigation. Molecular studies were done via PCR/RFLP analysis of angiotensin II subtype II receptor gene for monitoring their antioxidant activity through free iron chelation leading to inhibition of Fenton reaction.
Material and methods: Carnosine was labeled by mixing 4 mg with 30 mg glucose and 25 g SnCl2.2H2O, followed by pertechnetate and stand at room temperature for 60 minutes. Minor modification was done to prepare 99mTc(V)-DMSA tracer in one step, by adding pertechnetate solution to the lyophilized kit contains 1mg DMSA, 0.1 mg SnCl2.2H2O, and 30 mg glucose at pH 9. The biodistribution of the two tracers in normal and tumor-induced mice. The molecular investigation of the anti-oxidant activity of both carnosine and DMSA in 6 Gy - irradiated rats using the anti-inflammatory angiotensin II subtype II receptor gene (AT2RG) as indicator.
Results: Carnosine and DMSA were labeled with Technetium-99m yielding 85% and 97%, respectively the ability of both tracers to localize in tumor sites but the priority to the 99mTc (V)-DMSA. Molecular studies showed strong antioxidant activity of carnosine but not enough to block radiation induced oxidative stress and Moderate antioxidant activity of DMSA was achieved by chelating free iron and iron released through oxidative stress. Maximum protection was achieved through the dual action of both DMSA and carnosine. Conclusion: moderate and high labeling yield were achieved for both 99mTc(V)DMSA and 99mTc-canosine respectively with higher selectivity of the former to tumor sites and maximum protection were achieved by the dual action of both chelating agents.
https://ejhm.journals.ekb.eg/article_17707_79e18361f24615ac60cc8c3cbd56bc5e.pdf
Carnosine / DMSA / Anti-oxidant / Technetium-99m Labeling
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
664
671
10.21608/ejhm.2007.17708
17708
Original Article
Histological and histochemical study of effects of arsenic on the liver of adult male rabbits
Ibrahim K. Ibrahim
1
Faculty Of Medicine, AL-Azhar University-Assuit
Introduction: Arsenic is an environmental toxicant and a human carcinogen. Epidemiology studies link human arsenic exposure to various diseases and cancers, including liver diseases and hepatocellular carcinoma. From this point of view, this study is designed to investigate the effects of arsenic histologically and histochemically on the liver of adult male rabbits.
Material and methods: twenty adult male rabbits were used and divided into four groups. Each group consisted of five rabbits, one group used as a control given an equivalent volume of distilled water and three groups given sodium arsenite in distilled water for 6, 12 and 18 weeks respectively. Paraffin sections from all groups prepared and stained by haematoxylin and eosin, and periodic acid-schiff.
Results: histological and histochemical changes in arsenic groups were marked vacuolation of hepatocytes with focal areas of hepatocellular degeneration. Loss of hepatic architecture was also observed. Marked dilatation and congestion of the central veins and mononuclear cellular infiltration were obvious. Quantitative analysis of intracellular glycogen stores of all groups using image analyzing system revealed gradual significant depletion of intracellular glycogen stores.
Conclusion: it was concluded that arsenic is a hepatotoxic agent in the adult male rabbits.
https://ejhm.journals.ekb.eg/article_17708_b63e30e01ce44003a2bb50c5ae10e6aa.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
672
684
10.21608/ejhm.2007.17709
17709
Original Article
Histological Study Of The Influence Of Bioactive Glass On Bone Healing (An Experimental Study On Rat Femur)
K. M.T. Eraba
1
Elsayed G. Khedr
2
Tarek A. Atia
3
Salah E. Mourad
4
Faculty Of Science, Department Of Physics Al Azhar University
Faculty Of Medicine, Departments Of Histology
Faculty Of Medicine, Departments Of Histology
Faculty Of Science, Department Of Anatomy,Al Azhar University
The objective is to study the histological influence of the bioactive glass on bone healing in surgically created holes in rat’s femur.
Methods: The two wall holes were made in the right femurs by 2-mm drill, filled with bioactive glass in the examined rats. Another two wall holes were made similarly in the right femurs of other group of rats without filling with bioactive glass and used as the control group. Animals were regularly examined over a period of five weeks for bone healing.
Result: Histological examination of both control and treated sites showed newly formed bone. The newly formed ostoid tissue was significantly increased in the treated holes in the form of foci of newly formed bone around and within the glass particles. In the control group, the junctional epithelium migrated up to the base of the hole.
Conclusion: The treated animals with bioactive glass had better healing than control. The bioactive glass particles have osteo-conductive property as well as osteo-stimulatory capacity. The graft material showed a promising inhibition of greater cementum deposition in the bone holes. We can use it in Osteosurgery as it can promote bone healing.
https://ejhm.journals.ekb.eg/article_17709_9ab6f8f64a28b56d94f0eae3b2bf47a1.pdf
Bioactive glass
bone hole
bone healing
histological changes
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
685
706
10.21608/ejhm.2007.17710
17710
Original Article
Studies of the Genotoxic and Histopathological effects of the Organophosphorous insecticide 'Profenofos' on white rats.
Fatma M. Hammam
1
Eman M. Abd el Mottaleb
2
Department Of Mammalian Toxicology, Central Agricultural Pesticides Laboratory, Agricultural Research Centre, Ministry Of Agriculture
Department of pathology, Animal Health Research Institute
Genotoxic effects of agricultural chemicals are of special concern because of their generally irreversible effects and the long latency associated with their manifestation. These effects include heritable genetic diseases, carcinogenesis, reproductive dysfunction and birth defects. The present study was carried out to investigate the effect of the organophosphorous insecticide "profenofos" on white albino rats. The rats were treated for 28 days with three different doses of profenofos (1/20 LD50, 1/40 LD50, and 1/80 LD50). Then the animals were left without treatments for 14 days for possible recovery. The genotoxic effect of the pesticide was evaluated by using the micronucleus assay in the bone marrow and polymorphism of glutathione S-transferase (GST) by polymerase chain reaction (PCR). The results demonstrated that the treatment with profenofos caused a significant increase in the frequencies of micronucleated polychromatic erythrocytes. Results of polymorphism of both GSTM1 and GSTT1 showed positive genotype in the control group. While the results of GSTT1 polymorphism in the treated rats showed positive genotype in all doses of profenofos. The GSTM1 polymorphism showed positive genotype in the high and medium doses (1/20 LD50 and 1/40 LD50) but not in the low dose (1/80 LD50), where the GSTM1 was null (negative) genotype. After the recovery period the polymorphism of GSTM1 and GSTT1 was found to be positive genotype, except with the low dose (1/80 LD50) showed null genotype for GSTM1 gene. The histopathological data showed that profenofos exhibited histopathological changes in liver, kidney, spleen and tests. Liver showed hepatic cell damage with degenerative changes. The kidney showed heamorrhages, edema, necrosis and glomeruli shrinkage. The spleen showed slight deplesion of the lymphocytes of the white pulp. The tests showed interstitial edema and severe necrosis of spermatogenesis. From these results we concluded that the profenofos exert genotoxic and histopathological effects on albino white rats.
https://ejhm.journals.ekb.eg/article_17710_1995815e0e8709d91d904102b8f6f4c0.pdf
Profenofos
micronucleus
GSTM1
GSTT1
Organophosphorous
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
707
712
10.21608/ejhm.2007.17711
17711
Original Article
Effects Of Body Weight On Some House Works Efficacy And Some Physiological Parameters Of Females
In this work the effect of excess body weight on house works and some physiological parameters were conducted on twelve females’ university students. The students were divided into a control (normal weight) group and experimental (overweight) group. The weakly cleaning ofthelivingroomwaschosenashousework. Aroomwaspreparedasalivingroomwithsome furniture like tables, chairs, a sofa and cupboard in addition to a carpet on the ground and curtains on the walls. Observation of complexion, tremors, behavior reflecting boredom , level and time of performance as well as any complaint as tiredness, back pain, leg pain. Some physiological parameters as pulse, blood pressure, respiratory rate forced respiratory capacity and expiratory time were measured.
The results showed that overweight has passive effect on time and level of performance; also it affects the circulatory functions more than the respiratory functions. So house works are not considered as hard work and not sufficient to reduce body weight in overweight women. Also house works can be tolerated and there is no need to restrict activity to guard against respiratory distress
https://ejhm.journals.ekb.eg/article_17711_00f6f61fff089ecdff71dbbb147ca940.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
713
725
10.21608/ejhm.2007.17712
17712
Original Article
Identification of Athletic Human Growth Hormone Gene Polymorphisms
Mohammed H Awwad
1
Hany A. Eldeeb
2
Department of Zoology, Faculty of Science, Benha University
Department of Training sports, Faculty of Physical Education, Monofia University.
Background: PCR/RFLP of Human growth hormone gene was used to differentiate among ten athletes.
Results: AatI, AviII, BsaI, HindII, and SacI restriction endonucleases gave high levels of intraspecific profile for all the studied gene of the ten athletes. Other enzymes including AvaI, Age and DraI were tested for their ability to differentiate these individuals.
Conclusion: The study demonstrated that the HGH gene contains useful genetic markers for the identification of athletes.
https://ejhm.journals.ekb.eg/article_17712_f235c828d1522f15f4ebb11fd3cffbbf.pdf
Polymorphisms
athletes
PCR/RFLPs
HGH gene
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
726
731
10.21608/ejhm.2007.17713
17713
Original Article
Pilonidal disease simple pathogenesis but complex management
Mohammed Hassan
1
Amr Aiad
2
Wael L. Tobar
3
Islam Gamal
4
Ayman Refaat
5
Department of Surgery Cairo University
Department of Surgery Cairo University
Department of Surgery Cairo University
Department of Surgery Cairo University
Department of Surgery, Beni-Suef University
Design: retrospective study Patients & Methods: the study was conducted on 75 patients suffering PND with a male
to female ratio 59 to 16 done between May 2004 to June 2007 with a follow up range 8-13 and a median of 10.7 months. Sixty four patients had sacrococcygeal disease, 9 patients had umbilical disease, and 1 patient had suprapubic while 1 patient had axillary disease. For sacrococcygeal disease Limberg operation was done in 26.5% (n=17), abscess drainage and curettage in 31.2% (n=20), Bascom operation in 23% (n=15) while conservative treatment in 31.2% (n=20).For umbilical disease, omphalectomy was done in all cases (n=9). Conservative treatment was done for suprapubic disease while excision followed by primary closure was done in axillary disease (n=1) after failure of conservation.
Aim: to evaluate different modalities in treating pilonidal disease (PND)
Results: For sacrococcygeal disease, patients who received Limberg procedure (n=17) showed complete resolution in 88.2% (n=15) with recurrence rate 11.8% (n=2). Those who received Bascom operation showed complete resolution after all procedures with no recurrence during the follow up period. Patients who received conservative treatment (n=20) showed a success rate of 70% (n=14) with recurrence rate of 30% (n=6) who received Bascom procedure later for their recurrence. For umbilical, suprapubic and axillary disease; omphalectomy, conservative treatment and excision with primary closure were used respectively with no complications encountered.
Conclusion: For sacrococcygeal disease, Bascom operation was found to be superior over other modalities with respect to smooth postoperative period and early healing while in umbilical disease conservation shows higher success rate.
https://ejhm.journals.ekb.eg/article_17713_61f8c81836154ca735a40c1b93c68857.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
732
737
10.21608/ejhm.2007.17714
17714
Original Article
The Prevalence of Pediculosis Capitis In Primary School Children In Assuit Governorate ( A Socioeconomic Study )
Amer Abu El Enin
1
Ali Osman
2
The Departments of Dermatology &Venereology Faculty of Medicine. AL – Azhar university .
Public Health Faculty of Medicine. AL – Azhar university .
This study was carried out in 2005 to determine the prevalence of pediculosis capitis and some risk factors among primary – school pupils in Assuit .We selected 1200 pupils (53% girls) from primary schools by random sampling. Their hair was examined for head rate of infestation: 45 (3.8%) were infected with lice, 43 (95.5%) girls and 2 ( 4.5%) boys. The highest louse of infestation was in 9 – years olds. There was a significant relationship between head louse infestation and sex ( P < 0.0001), age( P<ٍ0.05 ), parents education ( P<0.0001), father's job (P< 0.01), family size (P< 0.01), length of hair ( P< 0.0001) and having separate bathing facilities in the house (P< 0.0001).
https://ejhm.journals.ekb.eg/article_17714_5fbda3c85fb7dc900436bbdfe77d1b44.pdf
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
738
744
10.21608/ejhm.2007.17715
17715
Original Article
Tinea Capitis In Assuit Governorate: (A Clinical and Mycological Study)
Amer Abu El – Enin
1
Mohamed Khedr
2
Alaa Abu El-Ata
3
Departments of Dermatology and Venereology Faculty of Medicine- Al-Azhar University.
Clinical pathology Faculty of Medicine- Al-Azhar University.
Microbiology Faculty of Medicine- Al-Azhar University.
Tinea capitis is a common condition seen by clinician in all setting through out the world. Several investigations of the various superficial mycosewere carried out in Egypt, but they were mainly confined to urban areas.
In the present work, the predominant species causing dermatophtosis in Assuit Governorate were investigated, this is represented in rural and partially suburban communities. A clinical and mycological study was done for hundred patients attending the Dermatology Out- patient Clinic in Al- Azhar University Hospital in Assuit City. The ages of patient ranged from 3 to 20 years .
60% of cases were between 6-10 years of ages.
Males were affected more than females in a ratio of 3: 1
60% of cases were from rural areas. Positive history of contact with animals was
present in 40%.
The most common clinical variety was scaly type 55% followed by black dot 22%,
kerion 20%, and lastly favus 3%.
Direct microscopic examination was + ve in 80% of cases. Positive culture results were
obtained in 90% of cases.
The results indicated that Zoophilic dermatophytes was the predominant causative
species in these regions. Five dermatophytes were identified namely: M.canis was the most frequently isolated organism from the clinical varieties of dermatophytosis (55%) . followed by T. violaceum (20%). T. rubrum 12.8% , M. gypseum (10%) and lastly T.soudanense (2.2%).
https://ejhm.journals.ekb.eg/article_17715_73545bea4453174c9633000086222784.pdf
Tinea capitis – Mycology
eng
Pan Arab League of Continuous Medical Education
The Egyptian Journal of Hospital Medicine
1687-2002
2090-7125
2007-10-01
29
1
492
510
10.21608/ejhm.2007.19575
19575
Original Article
Histological, Scanning And Transmission Electron Microscopic Studies On The Possible Protective Role Of Ginger Extract Against Acrylamide Induced Intestinal Damage In Mice
Hala Galal El-Tantawi
1
Department Of Zoology –Faculty Of Science - Ain Shams University
Objective: This study was carried out to evaluate the protective effect of ginger Zingeber officinale extract (ZOE) against the acrylamide (AC) which is an industrial chemical used in water treatment and it is synthesized during cooking of starch food at high temperature. Method: Thirty adult male albino mice, each weighs 20-25 g were divided into three groups (10 mice/group): (I)control group. (II)acrylamide treated group. (III) acrylamide & ginger group. Acrylamide was given to experimental animals in the drinking water at a nonlethal dose of 200 p.p.m for 10 weeks (3 days/week). Ginger extract was orally administrated at 50 mg/L (~5 ml/day) for 10 weeks (3 days/week). The ileum samples were collected for light microscope study and for scanning and transmission electron microscope examination. Results: This study revealed that acrylamide induces pathological changes of the ileum of the treated mice specially the absorptive epithelial cells. The scanning electron microscopic study revealed damage of the ileal villi, some red blood corpuscles appeared at the site of damage. The transmission electron microscopic examination clearly demonstrated degeneration of most cell organelles as mitochondria, deterioration and degranulation of the rough endoplasmic reticulum, dilatation of Golgi apparatus. Conclusion: The administration of ginger extract decreased the histological alterations and ensuring the anti-inflammatory, and antitoxic effects of ZOE at its chosen dosage level.
https://ejhm.journals.ekb.eg/article_19575_deb0a884e83e7e32c080350f9075d30e.pdf
Acrylamide – ginger- ileum