@article { author = {Al-Shafei, Amal A. and El-Nashar, Thanaa M. and Ghoneame, Yousr M. and Selim, Fatma M. and Ahmed, Gamal N.}, title = {Age and gender specific patterns of microfilaremia and circulating antigen with specific lymphocyte proliferative responses in Wucherereia bancrofti filariasis.}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {1-11}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18752}, abstract = {A population from a W. bancrofti- endemic area was evaluated by use of microfilaria(Mf) and adult worm circulating antigen(CAg) enzyme linked immunosorbent assay to demonstrate the relation of these two parameters to age , gender and the clinical status (symptomatic or asymptomatic) of the examined individuals. In addition, antigen-driven T cell proliferation was measured to study the effect of the clinical status and / or infection status on the host responsiveness in lymphatic filariasis. The results showed that the prevalence rates of each of the two markers are clearly age dependent and were significantly higher in males than females. The antigen prevalence rate was consistently higher than the microfilaria prevalence rate in all age groups. Among the asymptomatic and symptomatic patients, the direct relationship between Mf+ and CAg+ remained intact regardless of clinical status. Individuals positive for CAg had significantly lower lymphocyte proliferation responses to parasite antigen than did CAg-negative people. This study re-emphasizes the importance of CAg measurements for accurately assessing the clinical status of filariasis and demonstrates that the presence or absence of active infection, not clinical status is most closely associated with the immune responsiveness in lymphatic filariasis.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18752.html}, eprint = {https://ejhm.journals.ekb.eg/article_18752_0eb8b878e8ec81aeccb3e9c88c999d3c.pdf} } @article { author = {Abou Hashem, Safwat E. and Sleim, Kariman El-Houssieni and Sleim, Soad Abdalla and El-Gendy, Hesham and Abd-El-Aal, Ahmed A.}, title = {The Effect of The Specific Phosphodiesterase Type V Inhibitor, “Sildenafil Citrate”, on Alloxan-Induced Diabetic Rabbit Corpus Cavernosal Smooth Muscle}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {12-27}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18753}, abstract = {This study was carried out to investigate the effect of sildenafil (S) together with the influence of sodium nitroprusside (SNP) and acetylcholine (Ach) on isolated strips of rabbit corpus cavernosum. The in vitro effects of sildenafil on non-adrenergic, noncholinergic and nitric oxide (NO)-mediated cavernosal smooth muscle relaxation in diabetic rabbits, were also investigated. Diabetes mellitus was induced in adult male New Zealand white rabbits with alloxan (65 mg/kg, i.v). Cavernosal strips from agematched control and 5 months diabetic animals were mounted in organ baths containing Krebs-bicarbonate solution (pH 7.4, bubbled continueously with a gas mixture of 95% O2 plus 5% CO2, and maintained at 37oC. Isolated strips of control rabbit corpus cavernosum were stimulated isotonically with phenylephrine (10-5M) and relaxations were induced using increasing concentrations of Ach, SNP and S alone and in combination. Also, relaxation responses of control and diabetic strips precontracted with phenylephrine (10-5M) to electrical field stimulation (EFS, 4-20 Hz) or SNP (10-9 – 10-3M) were assessed in the absence and presence of a submaximal dose of sildenafil (10-6M). It was found that Ach, SNP and S relaxed the control rabbit corpus cavernosal strips in a dose-dependent manner. The relaxant effect of the test agents were ranked (from least inhibitory to most inhibitory) as follows: S, Ach plus S, SNP, SNP plus S, Ach. Moreover, it was observed that SNP-stimulated relaxations were significantly impaired in the corpus cavernosum from diabetic group compared to age-matched controls. Sildenafil (10-6M) significantly enhanced SNP-stimulated relaxation in control and diabetic groups. Electrical field stimulation-mediated relaxations of the corpus cavernosum were significantly impaired after 5 months diabetes mellitus and enhanced by sildenafil (10-6M). In conclusion, sildenafil enhances the relaxing effect of both SNP and Ach on the phenylephrine-induced contraction of rabbit cavernosal tissue. It could also be suggested that the impairment of NO-mediated relaxation of the diabetic corpus cavernosum reflect, at least in part, a defect in guanyl cyclase activity. These findings support the use of sildenafil as an effective, orally administered, treatment for diabetic erectile dysfunction.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18753.html}, eprint = {https://ejhm.journals.ekb.eg/article_18753_31a30ffae543cb26df0df556c05567f4.pdf} } @article { author = {Moustafa, Nehal A. and Tohamey, Amera}, title = {Ginsing Pre-Treatment Lessens The Acute Testis Injury Of Rats Induced By Thioacetamide}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {28-47}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18754}, abstract = {This work aimed to elucidate the protective action of panax ginseng roots against hazardous effects of thioacetamide{TA} intoxication .Oral administration of thioacetamide (300mg/kg)dissolved in drinking water for 12 weeks greatly affected liver and testis .This appeared from significant increase in serum alkaline phosphatase ,serum total acid phosphatase and serum prostate acid phosphatase . Previous starvation for 48 and 72 hrs immediately prior to TA administration strongly potentiated the effects of TA on the serum , testis and liver plasma membrane parameteres . The testis and liver damage may be one of the reasons of the cell death in TA intoxicated rat testis and liver. However, oral administration of Panax ginseng roots (117mg /kg.) for a period of 10 days prior to TA administration performed protective effect against TA toxicity. The obtained results showed that pre-treatment with Panax ginseng roots significantly modulated the changes in the physiological pathway induced by TA intoxication, and that Panax ginseng roots manifested marked improvement in the liver function and the structure of testis and epididymis. Also pre-treatment with ginseng caused a significant reduction in the deteriorated cells and this may support the protection against cellular damaging action.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18754.html}, eprint = {https://ejhm.journals.ekb.eg/article_18754_f9e3236ed990b504b7606e9a84eadf43.pdf} } @article { author = {El-Shazly, M. H. and Ghaffar, Nagwa A.}, title = {Tension-Free Vaginal Tape For Treating Stress Urinary Incontinence ( Types I And Ii ) : Experience With 32 Cases}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {48-55}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18755}, abstract = {Objectives: To evaluate the clinical outcome of using tension-free vaginal tape (TVT) for treating types I and II genuine stress urinary incontinence. Patients and Methods: The study comprised a total of 32 women with genuine stress urinary incontinence types I and II treated with TVT between 1999 and 2002. The mean follow-up period was 19.4 months. Results: The success rate was 87.5 % (28/32 patients). The cure rate was durable in the majority of successful cases 26/28 (92.9%) during the one year follow-up period except for 2/28 (7.1%) women in whom stress urinary incontinence recurred in a milder form. Postoperative retention of urine occurred in 2/32 (6.3%) patients, which was transient and responded well to urethral dilatation under local anaesthesia. Details of the clinical outcome variables and complications are represented. Conclusion: The TVT procedure is an effective and minimally invasive alternative to surgical options for managing types I and II stress urinary incontinence. The complication rates are low and hospital stay is short and the procedure is feasible under local anaesthesia}, keywords = {stress incontinence,TVT,tension-free vaginal tape}, url = {https://ejhm.journals.ekb.eg/article_18755.html}, eprint = {https://ejhm.journals.ekb.eg/article_18755_3b6d4b8255fd31305c1ae95cb01cdd23.pdf} } @article { author = {El Shazly, M. H. and Ghafar, Nagwa A.}, title = {Tension-Free Vaginal Tape And Burch Colposuspension For Managing Stress Urinary Incontinence ( Types I And Ii ): A Comparative Clinical Trial}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {56-64}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18758}, abstract = {Objectives: To conduct a retrospective study of the clinical outcomes between tension-free vaginal tape (TVT) and Burch colposuspension for managing types I and II stress urinary incontinence (SUI) in women. Patients and methods: Between 1999 and 2002 a total of 32 TVT procedures and 43 Burch colposuspensions were used for managing 75 women with types SUI (types I and II) for a minimum follow-up period of one year. Results: The overall short-term (one year follow-up) success rates for TVT were 87.5% versus 90.7% for Burch colposuspension. The cure rates were sustained in the vast majority of patients during the short-term follow-up period (one year). Postoperative transient obstructive voiding dysfunctions were significantly more severe and common in Burch colposuspension (25.6%) versus (15.6%) for TVT. Further details of the clinical outcome and complications are presented. Conclusion: Both options are effective in managing SUI (types I and II) with comparable success rates. The success rate is marginally higher for the Burch colposuspension. However, the TVT procedure had shorter operative time, shorter hospital stay, fewer and milder complication rate, and is feasible under local anaesthesia. Nevertheless, the long-term results regarding the durability of the improvement rates are still to be further awaited for the TVT procedure.}, keywords = {stress incontinence,tension-free vaginal tape,colposuspension}, url = {https://ejhm.journals.ekb.eg/article_18758.html}, eprint = {https://ejhm.journals.ekb.eg/article_18758_d6586e9f8f75f11bece915a83f9632b1.pdf} } @article { author = {El-Mahdy, Magda I.M. and Morsy, Mona M.}, title = {Angiotensin-converting enzyme gene polymorphism in patients with Essential hypertension}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {65-73}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18759}, abstract = {Several genetic investigations have been attempted to elucidate the association of angiotensin-converting enzyme (ACE) gene polymorphism and essential hypertension. This study was conducted to investigate the frequency of ACE gene insertion/deletion (I/D) polymorphism in patients with essential hypertension (EH). The study included one hundred patients with essential hypertension and seventy age and sex matched healthy individuals as a control group. The patients and control group were subjected to routine investigations, assay of serum cholesterol, triglycerides, high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) and assay of ACE gene I/D polymorphism using real-time polymerase chain reaction (PCR). The results of the study showed that the frequency of DD,ID and II genotypes were 42%, 44% and 14 % respectively in hypertensive group and 30%, 50% and 14 % respectively in control group with significantly higher frequency of DD genotype in patients as compared to the control group (p<0.05). There was a significant association between DD genotype and hypertension, as there was significant increase in both systolic and diastolic blood pressure in patients with DD genotype as compared to other genotypes. Serum cholesterol , HDL-C and LDL –C levels showed significant increase in patients as compared to the control group (P <0.001, P <0.001 and P <0.001: respectively ) Also, serum Cholesterol and LDL-C levels showed significant increase in patients with DD and ID genotypes as compared to II genotype , while triglycerides and HDL-C didn’t show differences between the three genotypes. It was concluded that the DD genotype of ACE gene showed significantly higher frequency among patients with essential hypertension as compared to the normal subjects and that DD genotype was associated with significantly higher blood pressure as compared to ID and II genotypes. Also, DD genotype was associated with significantly higher serum cholesterol and LDL-C as compared to II genotype. This polymorphism in the ACE gene may contribute to the pathogenesis and severity of essential hypertension and may help in selection of anti-hypertensive drugs.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18759.html}, eprint = {https://ejhm.journals.ekb.eg/article_18759_478186211a2d97854d15b84556d67206.pdf} } @article { author = {Khedr, El Sayed Galal and Yossef, Ahmed H.}, title = {Evaluation Of Subcutaneous Tissue Response To Implanted Samples Of Acrylic Resin Mixed With Metal Fillers}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {74-84}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18761}, abstract = {This study was conducted to to study the subcutaneous tissue response as well as some properties of of heat acrylic resin mixed with Aluminum (Al) and Silver (Ag). (2%;3%;5%;10%) concentrations of Al and Ag were added separately to heat cured acrylic resin and the mechanical properties of themixture were examined by the common Elson technique . The addition of 2% of either Al and Ag to heat acrylic resin increased its transverse strength as well as its impact strength ; while 5% and 10% cocentrations reduced both the transverse and the impact strengths of the resin . Therefore 2% concentrationwas chosen for subcutaneous tissue implantation. Three pellets of acrylic resin one containing Al ;one containing Ag and the third was free from both elements as a control were each implanted in the subcutaneous c.t. of twenty male albino rats. A thick connective tissue capsule surrounding each pellet was formed . Al containing acrylic resin was surrounded with a thicker capsule formed of collagen fibers infiltrated with many inflammatory cells ;while the connective tissue reaction against the pure acrylic resin and Ag acrylic resin was minimal in the form of thin capsule formed of collagen fibers; fibroblasts and few inflammatory cells . Measurements of the capsule thickness and the number of inflammmayory cells infiltrating the capsule showed a significant increase in Al group than in Ag and control groups .In conclusion the addition of 2% of either Al and Ag to heat acrylic resin improved its strength but Ag containing heat acrylic resin was more tolerable to tissue.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18761.html}, eprint = {https://ejhm.journals.ekb.eg/article_18761_0687eedba36771c44a7c76bab8365c8f.pdf} } @article { author = {Helal, Eman G. E. and Sharaf, Hafiza A. and Mattar, Fathy E.}, title = {Anti – diabetic and anti-obesity effects of plant extract from Cleome droserifolia (SAMWA)}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {85-101}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18766}, abstract = {In this work the mechanism of the hypoglycemic effect of an aqueous extract from Cleome droserifolia (SAMWA) was studied in a group of glucose intolerant senile rats and compared with normal senile control rats. The plant extract significantly suppressed the rise in blood glucose concentration. The hypoglycemic effect of the plant extract without increasing insulin secretion was explained by: 1) potentiation of peripheral and hepatic insulin sensitivity, 2) by diminishing intestinal glucose absorption, which was evident by blunting plasma glucose levels throughout the oral glucose challenge. This was accompanied by a significant decrease of liver glycogen content and a highly significant increase in leptin concentration which may suggest an anti-obesity role for the plant extracts. Plant extracts also decreased serum urea nitrogen. No changes on liver or heart function were observed. In histological sections of liver and kidney minor changes were noticed. These extracts might prove to have a promising therapeutic value in the treatment of diabetes mellitus. Besides its suppression to hepatic glucose output, SAMWA beneficial therapeutic finding in favor of the plant as a replacement for insulin which is the most important drug that brings bout this effect.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18766.html}, eprint = {https://ejhm.journals.ekb.eg/article_18766_3c5da180577b6fc2588ea22bb83ee4d0.pdf} } @article { author = {Soliman, Maha G.}, title = {Aqueous Extract Of Camellia Sinuses Shows Immunological And Histological Changes In Induced Inflammatory Animal Models}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {101-111}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18767}, abstract = {The present study investigated the effect of green tea (Camellia sinuses) aqueous extract on the inflammatory response induced by Carrageenan (CGN) (1%) in Sprague dawaly rats. 48 rats were equally divided into 6 groups:control, green tea drinking, Carrageenan (1.2%) treated for 24 hours, green tea - Carrageenan treated for 24 hours, CGN treated for 72 hours, green tea - CGN treated for 72 hours. On the last day of drinking green tea aqueous extract, inflammation was induced to rats by Carrageenan . Twenty-four and seventy-two hours after CGN challenge, blood samples were withdrawn and animals were sacrificed. Animals which were injected with CGN had shown highly significant leucocytosis, monocytosis and eosinophilia. More reticuloendothelial organ damages like severe inflammation, cellular lymphocytic infilteration and congestion were distinguished in 72 hours animal group. Green teadrinking and CGN treated groups showed a significant improvement in reticuloendothelial organs such as thymus gland, spleen and liver. A histopathological improvement of these organs was observed in green tea and CGN 72 hours treated group more than that group which treated for 24 hours. This group showed also a significant drop in total leucocyte count and peritoneal fluid neutrophils while a significant increase of bone marrow lymphocyte count was observed when compared with the CGN treated animal group. A significant modulation in differential leucocytic count especially the drop in lymphocytic and eosinophilic percentage occurred. This was associated with lower serum globulin and immunoglobulin G (IgG) in green teadrinking-CGN treated animal group in comparison to CGN treated animal groups. This study explains the immunomodulatory role played by green tea in response to inflammatory immunostimulant agent.}, keywords = {}, url = {https://ejhm.journals.ekb.eg/article_18767.html}, eprint = {https://ejhm.journals.ekb.eg/article_18767_b9388c42456865fa2518dd7880848e8f.pdf} } @article { author = {Awwad, Mohammed H. and Abou El kheir, Sahar M. and Lashien, Gehan H.}, title = {Molecular Identification of Three Argas Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Small-Subunit Ribosomal RNA Gene}, journal = {The Egyptian Journal of Hospital Medicine}, volume = {9}, number = {1}, pages = {112-121}, year = {2002}, publisher = {Pan Arab League of Continuous Medical Education}, issn = {1687-2002}, eissn = {2090-7125}, doi = {10.21608/ejhm.2002.18769}, abstract = {The polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLPs) of SrRNA gene of Argas species (~1800 bp) is a very useful technique for differentiation of Argas persicus, Argas hermanni and Argas arboreus species. The PCR/RFLPs profile of EaeI and EcoRI restriction endonucleases were highly characteristic of the genetic interspecific levels and low genetic intraspecific levels of the three species. Other enzymes proved that A. persicus and A. hermanni may be a single or monophyletic species (SacII and SstII restriction endonucleases). AvaII restriction enzyme showed that A. hermanni and A. arboreus could be a monophyletic species. AvaI restriction endonuclease was the only restriction enzyme to prove that the three Argas species may be polyphyletic species and identified uniquely by this enzyme.}, keywords = {identification,Argas,RFLPs,SrRNA gene}, url = {https://ejhm.journals.ekb.eg/article_18769.html}, eprint = {https://ejhm.journals.ekb.eg/article_18769_f564cc4fe136f63bd344c74ca09c877a.pdf} }